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Draft genome sequence and overview of the purple non sulfur bacterium Rhodopseudomonas palustris 42OL


Rhodopseudomonas palustris strain 42OL was isolated in 1973 from a sugar refinery waste treatment pond. The strain has been prevalently used for hydrogen production processes using a wide variety of waste-derived substrates, and cultured both indoors and outdoors, either freely suspended or immobilized. R. palustris 42OL was suitable for many other applications and capable of growing in very different culturing conditions, revealing a wide metabolic versatility. The analysis of the genome sequence allowed to identify the metabolic pathways for hydrogen and poly-β-hydroxy-butyrate production, and confirmed the ability of using a wide range of organic acids as substrates.


Rhodopseudomonas palustris is a PNSB belonging to the class Alphaproteobacteria . According to Imhoff et al. [1], the term PNSB is used to indicate a physiological group of anoxygenic phototrophic bacteria, affiliated to both Alphaproteobacteria and Betaproteobacteria , containing photosynthethic pigments and able to carry out anoxygenic photosynthesis.

Strains of R. palustris have been isolated from a variety of different environments, from eutrophic lagoons to moist soils, from freshwater ponds to marine coastal sediments [24]. The very wide spread of R. palustris throughout a variety of habitats is due to its extreme metabolic versatility, with all modes of metabolism represented (autotrophic, heterotrophic, organotrophic, litotrophic, chemotrophic and phototrophic); moreover, the organism is a facultative anaerobe [5].

All PNSBs are characterized by the ability of carrying out anoxygenic photosynthesis; in the presence of oxygen, photosynthesis is inhibited and a number of PNSBs are able to carry out respiration [4]. Under anaerobic conditions, and subject to light irradiation, PNSBs are able to fix nitrogen via nitrogenase; hydrogen is produced as a by-product of nitrogen fixation. Among PNSBs, R. palustris is considered a model organism for studying biological hydrogen production, due to its capacity of efficiently producing hydrogen during organic wastes degradation [6].

R. palustris 42OL has been used previously for hydrogen production processes under various conditions [722], i.e., with different substrates, and cultured indoors and outdoors, using freely suspended or immobilized cells. However, its first application was in mixed culture in wastewater treatment [23]. Its biomass was evaluated for SCP accumulation and amino acid composition [24]. The accumulation of PHB and its connection to hydrogen production were investigated [8, 11, 25]. More recently, the biomass of R. palustris 42OL was also used as a biosorbent for metal removal from wastewaters [26, 27]. Furthermore, the NMR and X-ray structures of its 7Fe-8S ferredoxin and cytochrome c 2 were studied [2830]. The latest application of the strain was for antibiotic delivery though liposomes formed with its lipids [31].

The long history and the versatility of this particular strain render it a very good candidate for further investigating the basis of its ability to acclimate to very different culturing conditions.

Organism information

Classification and features

R. palustris 42OL was isolated in 1973 from a catch-basin collecting the effluents of a sugar refinery waste treatment pond, in Castiglion Fiorentino (AR), Italy. The enrichment was carried out aimed at selecting waste degrading phototrophs. The isolated microorganism has been stored since 1973 at CSMA Collection (WDCM number 147) under the collection name CSMA73/42, growing anaerobically on solid RPN medium [32] with malate 2 g L-1 as the carbon source and supplemented with 0.4 g L-1 of yeast extract. The general features of the isolate are reported in Table 1.

Table 1 Classification and general features of Rhodopseudomonas palustris 42OL, according to MIGS standards [45]

The isolate 42OL was firstly assigned morphologically to R. palustris . Phylogenetic analysis performed subsequently (unpublished results) by comparing 16S rRNA gene sequences revealed that the isolate might be indeed affiliated to the species R. palustris . With the present work, a further phylogenetic analysis was conducted and, as shown in the phylogenetic tree in Fig. 1, confirms its affiliation.

Fig. 1

Maximum Likelihood dendrogram based on 16S rRNA gene; Bar = 1.1 indicates the nucleotides substitution rate. Numbers at the nodes indicate bootstrap values after 500 random replicates. Numbers after strain name indicate the GI code. Sequences have been selected after BLAST Explorer [56] search for most similar sequences present in GenBank database. Dendrogram has been constructed by using the Maximum Likelihood algorithm with default options present in web server [56]. Strains TIE-1 and DX-1 have completely sequenced genomes; ATCC17001 is the type strain and is indicated asT

R. palustris 42OL is a Gram-negative rod shaped bacterium, of 0.6–1.2 μm (see Fig. 2a). It replicates by polar budding (Fig. 2b) and new cells present one single flagellum that is lost in the subsequent phases of cell cycle [33, 34]. Its photosynthetic apparatus is located on lamellar ICMs, clearly visible in Fig. 2c, d, as characteristic of this species [2]. The major carotenoid molecules that are present in the photosynthetic apparatus of this strain are spirilloxanthin, rhodopin, rhodovibrin, anhydrorhodovibrin and lycopene (our unpublished results).

Fig. 2

Electron micrographs of Rhodopseudomonas palustris 42OL grown on RPN medium; a whole cell, longitudinal section; b cell during polar budding (white arrow), longitudinal section; c whole cell containing PHB granules. d lamellar ICMs in whole cells, transversal section; PHB, poly-β-hydroxybutyrate granules; ICM, intra-cytoplasmic membranes; P, polyphosphate granules; CW, cell wall

The first characterization of R. palustris 42OL was in terms of protein accumulation and amino acid composition on different carbon and nitrogen sources. Malate and ethanol were tested as carbon sources, both under nitrogen fixing (N2 sparged) and non-fixing (NH4 + supplied) conditions. A significantly lower amount of protein was produced in presence of ethanol, while the nitrogen source did not have any effect. However, both nitrogen and carbon sources significantly influenced the amino acid composition of the protein biomass [24].

The carbon metabolism of the strain was investigated in terms of substrates that could be used for growth and hydrogen production. Short chain fatty acids such as acetate, pyruvate, lactate, malate and succinate were found to be photodissimilated by the strain with substrate conversion efficiency of 40, 52, 61, 56, and 67 %, respectively [35]. Butyrate was found to be suitable for growth and hydrogen production but with the significantly lower substrate-to-hydrogen conversion efficiency of 9 % (unpublished data).

Vincenzini et al. [36] characterized nitrogenase activity of R. palustris 42OL in terms of hydrogen production and acetylene reduction with different atmosphere composition and at different pH values. The optimal conditions for hydrogen production were under Argon gas as the atmosphere, for early logarithmic stage cells, at pH 6.8. The authors also demonstrated the presence of a hydrogenase enzyme, recycling the hydrogen produced during late logarithmic and light-limited stage.

Evidences of its suitability for the treatment of wastes combined with hydrogen production were reported [35], using wastewaters deriving from a sugar refinery and a paper mill. The same strain was shown to grow and produce hydrogen on different substrates such as vegetable wastes [15], olive mill wastewaters [12, 13] and dark fermentation saline effluents [14].

PHB is synthetized as a reservoir for reducing equivalents by the strain, in this way competing with hydrogen production [25], especially when grown on acetate [25], or when subject to phosphorus starvation [11]; in this condition, R. palustris 42OL could accumulate up to 18 % w/w of PHB on cell dry weight. R. palustris 42OL accumulates PHB in large amorphous granules, as shown in Fig. 2. Glycogen is synthetized as well as carbon and energy reserve [25].

Another relevant characteristic of this strain is the possibility of cultivation outdoors, under light/dark cycles both for biomass [37, 38] and hydrogen production [10, 11, 22], with an impressive capability of the photosystem to take advantage of the high light conditions that take place during the central hours of the day [21].

Genome sequencing information

Genome project history

The organism was selected for genome sequencing on the basis of its metabolic versatility and biotechnological relevance, as witnessed by its long history and by the diversity of applications. Project information is available from the Genomes OnLine Database [39], under the GOLD study ID Gs0114708. The WGS sequence is deposited in GenBank (LCZM00000000).

Growth conditions and genomic DNA preparation

R. palustris 42OL (CSMA73/42) was maintained anaerobically on solid RPN medium [32] with malate 2 g L-1 as the carbon source and supplemented with 0.4 g L-1 of yeast extract. For the extraction of genomic DNA a single colony of cells grown on agar plate was harvested and cultured anaerobically on the same liquid medium in 20 mL sealed glass tubes, at room temperature with a light irradiance of 80 μmol of photons m-2 s-1. Cultures were then transferred into 100 ml round bottles and the headspace was exchanged with Argon gas for anaerobiosis. Cells were harvested at an OD660 = 0.5, in mid-logarithmic phase, pelleted and stored at −20 °C. DNA was isolated from the cells using a CTAB bacterial genomic DNA isolation method, and checked on agarose gel. The genomic DNA purity was assessed by spectrophotometric measurements [40].

Genome sequencing and assembly

The draft genome sequence was generated using the Illumina technology. A Nextera XT DNA library was constructed and sequenced using Illumina MiSeq platform which generated 23,625,870 reads. After trimming, a total of 7,574,912 paired end reads were obtained and assembled into 308 high quality contigs (larger than 5419 bp each) using Abyss 1.0.0 software present on the Galaxy Orione server [41]. A summary of the project information is shown in Table 2.

Table 2 Project information

Genome annotation

Genes were identified using the prokaryotic genome annotation software Prokka 1.4.0 [42] (Galaxy Orione server [40]). For gene finding and translation, Prokka makes use of the program Prodigal [43]. Homology searching (BLAST, hmmscan) was then performed using the translated protein sequences as queries against a set of public databases (CDD, PFAM, TIGRFAM) as well as custom databases that come with Prokka. Additional gene prediction analysis and functional annotation were performed within the CBS Bioinformatics Tools platform developed by the Technical University of Denmark (Table 3).

Table 3 Genome statistics*

Genome properties

The genome of R. palustris 42OL resulted to be 5,128,858 bp in length with a GC content of about 65.74 % (Table 3). It was predicted to contain 4767 genes, 4715 of which coded for proteins and 52 for RNA (tRNA and rRNA). The majority of the predicted genes (68.74 %) could be assigned to one of of 25 functional COG categories whilst the 8.42 % of the remaining genes were annotated as hypothetical and 38.9 % as unknown function proteins. The distribution of genes into COGs functional categories is presented in Table 4.

Table 4 Number of genes associated with general COG functional categories

Insights from the genome sequence

The genome of R. palustris 42OL contained, as expected, genes related to nitrogen fixation (nif H, D, K, E, N, B, U, X, Q, W, Z), genes involved in carbon fixation (RubisCO), the complete tricarboxylic acid cycle, the glyoxylate shunt, a Embden-Meyerhof pathway, and a pentose phosphate pathway. Genes coding for the synthesis of glycogen and poly-β-hydroxyalkanoates as carbon storage polymers were also found, as well as genes related to the photosynthetic apparatus, similarly to all the other R. palustris strains so far sequenced.

The genome of R. palustris 42OL was analyzed in terms of synteny with other strains sequenced. The genome was found to be highly syntenic with those of other strains of R. palustris (data not shown). Exclusive reactions were then mapped on KEGG with respect to other R. palustris strains sequenced so far (BisA53, BisB18, BisB5, CGA009, Haa2, TIE1) by using DuctApe v 0.17.2 software [44]. Data obtained are reported in Table 5. The proteome size ranged between 4392 and 5242 protein coding genes, corresponding respectively to strains BisB5 and TIE1. The total number of reactions ranged between 2442 and 3012, respectively for strains BisA53 and CGA009. Strain Haa2 resulted to have the highest number of unique reactions.

Table 5 DuctApe analysis report


In this study, we characterized the genome of R. palustris strain 42OL isolated from a wastewater pond of a sugar refinery in 1973. Along the last four decades, this strain has been successfully used in a wide number of applications, from hydrogen production on wastewaters (its major application) to PHB production. The present genome analysis supported those findings.



Centro Studi Microrganismi Autotrofi


Cetyl trimethyl ammonium bromide


intra-cytoplasmic membrane


poly-β-hydroxy butyrate


purple non sulfur bacterium


single cell protein


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The authors gratefully acknowledge Professor Massimo Vincenzini, University of Florence, for sharing his experience and for having first elaborated the wide metabolic potentials of R. palustris 42OL strain.

The genome sequencing was performed by Francesca Crucianelli at Polo GGB, Perugia, Italy. The electron micrographs were performed by Maria Cristina Salvatici at CE.M.E., CNR, Florence, Italy.

Authors acknowledge the contribution given to this study by Ente Cassa di Risparmio di Firenze (ECRF) that supported AA with a fellowship in the frame of Florence Hydrolab2 Project.

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Correspondence to Roberto De Philippis.

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None of the authors has any competing interests in the manuscript.

Authors’ contributions

AA coordinated the study and the manuscript drafting. AA cultured the strain and analyzed electron micrographs. GS extracted the DNA. GS, AM, and LP performed all the bioinformatics analysis. AA, GS, AM and LP drafted the manuscript. All Authors discussed and revised the manuscript. All Authors commented on the manuscript before submission. All authors read and approved the final manuscript.

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Adessi, A., Spini, G., Presta, L. et al. Draft genome sequence and overview of the purple non sulfur bacterium Rhodopseudomonas palustris 42OL. Stand in Genomic Sci 11, 24 (2016).

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  • Rhodopseudomonas palustris 42OL
  • Purple non-sulfur bacteria
  • Hydrogen production
  • Wastewater treatment
  • PHB accumulation