- Short genome report
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Genome sequence of Oceanobacillus picturae strain S1, an halophilic bacterium first isolated in human gut
Standards in Genomic Sciences volume 10, Article number: 91 (2015)
Oceanobacillus picturae is a strain of a moderately halophilic bacterium, first isolated from a mural painting. We demonstrate, for the first time, the culture of human Oceanobacillus picturae, strain S1T, whose genome is described here, from a stool sample collected from a 25-year-old Saoudian healthy individual. We used a slightly modified standard culture medium adding 100 g/L of NaCl. We provide a short description of this strain including its MALDI-TOF spectrum, the main identification tool currently used in clinical microbiology. The 3,675,175 bp long genome exhibits a G + C content of 39.15 % and contains 3666 protein-coding and 157 RNA genes. The draft genome sequence of Oceanobacillus picturae has a similar size to the Oceanobacillus kimchii (respectively 3.67 Mb versus 3.83 Mb). The G + C content was higher compared with Oceanobacillus kimchii (respectively 39.15 % and 35.2 %). Oceanobacillus picturae shared almost identical number of genes (3823 genes versus 3879 genes), with a similar ratio of genes per Mb (1041 genes/Mb versus 1012 genes/Mb).
The genome sequencing of Oceanobacillus picturae strain S1 isolated for the first time in a human, will be added to the 778 genome projects from the gastrointestinal tract listed by the international consortium Human Microbiome Project.
A pure culture remains essential in microbiology. Nevertheless, metagenomics studies replaced culture methods entirely with regards to the exploration of complex ecosystems. The Human Microbiome Project (HMP) is an initiative with the goal of identifying and characterizing the microorganisms which are found in association with both healthy and diseased humans. To date (25 March 2015), 778 genome projects from the gastrointestinal tract are listed by HMP . Since 2012, we applied microbial culturomics (based on the multiplication of the culture condition with a rapid identification method by MALDI-TOF) in order to extend the human gut composition. Testing more than 500,000 colonies by MALDI-TOF, we isolated more than 700 different bacterial species including more than 90 new bacterial species and 180 previously known bacterial species but first isolated in humans . Each new bacterial species was described by taxonogenomics, a polyphasic approach adding genome sequencing and MALDI-TOF comparison in addition to classic phenotypic characteristics . In addition, in order to make the genome sequencing data available to the international scientific community, we propose the sequencing of the genomes of all bacterial species we isolated in humans, for which no genome sequencing was previously available . This will facilitate the future analysis of metagenomics studies. These strains are available for the scientific community (Collection de Souches de l’Unité des Rickettsies = CSUR). Herein, we report the genome sequencing of Oceanobacillus picturae strain S1 isolated for the first time in humans.
The genus Oceanobacillus was first described by Lu et al. in 2001  and was emended by Yumoto et al. in 2005 . These bacteria belong to the phylum Firmicutes , within the family Bacillaceae . This genus included 17 recognized species and two subspecies. These bacteria are motile Gram-positive rods, growing obligatory aerobically or facultative anaerobically. Some of them are moderately halophilic bacteria. Bacteria from the genus Oceanobacillus were isolated from diverse environmental samples [5–14], including deep-sea sediment cores , salt fields , fermented shrimp paste samples , soy sauce production equipment , and traditional Korean fermented food . Oceanobacillus picturae was originally described as Virgibacillus picturae in 2003 and was isolated from a mural painting from the Servilia tomb of the Roman necropolis at Carmona (Seville, Spain) . Lee et al. reclassified this species as Oceanobacillus picturae in 2006 . In addition to these validly published species, as a part of a large culturomics study , we isolated another Oceanobacillus species (“ Oceanobacillus massiliensis ”) from human fecal flora .
In this study we isolated for the O. picturae from humans for the first time. Strain S1 was isolated from a stool sample of a 25 year-old obese Saudi individual (BMI = 51 kg/m2) using a modified Columbia agar (Becton Dickinson, Pont de Claix, France) adding 100 g/L of NaCl. We described here the genome sequencing of this bacterium.
Classification and features
A stool specimen was collected from a 25-year-old Saudi obese patient. The patient gave informed and signed consent. The study and the assent procedure were approved by the Ethics Committees of the King Abdulaziz University, King Fahd medical Research Center, Saudi Arabia, under agreement number 014-CEGMR-2-ETH-P, and of the Institut Fédératif de Recherche 48, Faculty of Medicine, Marseille, France, under agreement number 09–022. The stool sample was preserved at −80 °C after collection and sent to Marseille. O. picturae strain S1T (Table 1) was isolated in December 2013 by aerobic cultivation on a culture medium consisting of a Columbia broth culture medium (Sigma-Aldrich, Saint-Quentin Fallavier, France) modified by the addtion of 100 g/L of NaCl with a pH adjusted to 7.5. O. picturae strain S1 had a 16S rRNA sequence similarity of 99.8 % with the reference strain O. picturae strain LMG19492T (Genbank accession number NR_028952) (Fig. 1). This strain was deposited in the CSUR (under number P887).
Strain S1 colonies were observed on sheep blood agar (Biomérieux, Marcy l’Etoile, France) after 24 h of aerobic incubation at 37 °C. The colonies were greyish, 3–4 mm in diameter. Gram staining revealed Gram-positive bacilli (Fig. 2) and electron microscopy performed using a Morgani 268D (Phillips) showed rods with a mean length of 1.5 μm and a mean width of 0.5 μm (Fig. 3). Optimal growth was observed at 37 °C and strain S1 grew only under aerobe conditions.
Extended feature descriptions
We added in the description the MALDI-TOF spectra of this bacterium. Indeed, mass spectrometry has become the reference identification method in clinical microbiology . Matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) MS protein analysis was carried out. Briefly, a pipette tip was used to pick one isolated bacterial colony from a culture agar plate and to spread it as a thin film on a MALDI-TOF target plate (Bruker Daltonics, Germany). Twelve distinct deposits were done for the strain S1 from twelve isolated colonies. After air-drying, 2 μl matrix solution (saturated solution of α-cyanohydroxycinnaminic acid in 50 % aqueous acetonitrile containing 2.5 % trifluoroacetic acid) per spot was applied. MALDI-TOF MS was conducted using the Microflex LT spectrometer (Bruker Daltonics). All spectra were recorded in the positive linear mode for the mass range of 2000 to 20,000 Da (parameter settings: ion source 1 (ISI), 20 kV; IS2, 18.5 kV; lens, 7 kV). A spectrum was obtained after 675 shots with variable laser power. The time of acquisition was between 30 s and 1 min per spot. The twelve spectra of strain S1 were imported into the MALDI BioTyper software (version 2.0, Bruker) and analyzed by standard pattern matching (with default parameter settings) against the main spectra of over 4108 bacteria including the spectra from the most closely related species including Oceanobacillus oncorhynchi CIP108867T, Oceanobacillus profundus CIP 109535T, Oceanobacillus chironomi CIP 109536T, Oceanobacillus iheyensis CIP 107618T, and Oceanobacillus oncorhynchi subsp. incaldanensis CIP 109235T, and Oceanobacillus sojae . In addition to these validly published species, we compared the O. picturae spectrum with spectra of Oceanobacillus massiliensis strain N’Diop and ‘ Oceanobacillus manasiensis’ (HG931336). The resulting score was > 2, matching with Oceanobacillus picturae CIP 108264 T. The identification method included the m/z from 3000 to 15,000 Da. For every spectrum, a maximum of 100 peaks were compared with spectra in the database. We added the spectrum from strain S1T to our database (Fig. 4).
Genome sequencing information
Genome project history
The O. picturae genome was sequenced as part of a culturomics study aiming to isolate all bacterial species colonizing the human gut  (Table 2). To the best of our knowledge, O. picturae represent the fifth genome sequenced into the Oceanobacillus genus and the first genome of O. picturae . The genome accession number is CCAX00000000 and consists of 5 contigs without gaps. Table 3 shows the project information and its association with MIGS version 2.0 compliance.
Growth conditions and genomic DNA preparation
O. picturae strain S1T (CSUR P1091 = DSM 28586) was grown at 37 °C in an aerobic atmosphere on ten Petri dishes. The bacteria were harvested and resuspended in 4 × 100 μL of TE buffer. Then, 200 μL of this suspension was diluted in 1 mL TE buffer for lysis treatment that included a 30- min incubation with 2.5 μg/μL lysozyme at 37 °C, followed by an overnight incubation with 20 μg/μL proteinase-K at 37 °C. Extracted DNA was then purified using 3 successive phenol-chloroform extractions and ethanol precipitation at −20 °C overnight. After centrifugation, the DNA was resuspended in 160 μL TE buffer. The yield and concentration were measured by the Quant-it Picogreen kit (Invitrogen) on the Genios-Tecan fluorometer at 88.6 ng/μl.
Genome sequencing and assembly
Genomic DNA of Oceanobacillus picturae was sequenced using MiSeq Technology (Illumina Inc, San Diego, CA, USA) with the mate pair strategy. The gDNA was barcoded in order to be mixed with 11 other projects with the Nextera Mate Pair sample prep kit (Illumina). The gDNA was quantified by a Qubit assay with the high sensitivity kit (Life technologies, Carlsbad, CA, USA) to 40.5 ng/μl. The mate pair library was prepared with 1 μg of genomic DNA using the Nextera mate pair Illumina guide. The genomic DNA sample was simultaneously fragmented and tagged with a mate pair junction adapter. The profile of the fragmentation was validated on an Agilent 2100 BioAnalyzer (Agilent Technologies Inc, Santa Clara, CA, USA) with a DNA 7500 labchip. The DNA fragments ranged in size from 1 kb up to 10 kb. No size selection was performed and only 14 ng of tagmented fragments were circularized. The circularized DNA was mechanically sheared to small fragments with an optimum at 696 bp on the Covaris device S2 in microtubes (Covaris, Woburn, MA, USA). The library profile was visualized on a High Sensitivity Bioanalyzer LabChip (Agilent Technologies Inc, Santa Clara, CA, USA). The libraries were normalized at 2 nM and pooled. After a denaturation step and dilution at 10pM, the pool of libraries was loaded onto the reagent cartridge and then onto the instrument along with the flow cell. Automated cluster generation and sequencing runs were performed in a single 42-h run in a 2x251-bp. Total information of 4.7 Gb was obtained from a 488 K/mm2 cluster density with a cluster passing quality control filters of 97.2 % (9,590,000 clusters). Within this run, the index representation for O. picturae was determined to be 11.16 %. Illumina reads were trimmed using Trimmomatic , then assembled through Spades software [33, 34]. Contigs obtained were combined together by SSpace  and Opera software v1.2  helped by GapFiller V1.10  to reduce the set. Some manual refinements using CLC Genomics v7 software cCLC bio, Aarhus, Denmark) and homemade tools improved the genome. Finally, the draft genome of Oceanobacillus picturae consists of 5 contigs without gaps.
Non-coding genes and miscellaneous features were predicted using RNAmmer , ARAGORN , Rfam , PFAM , Infernal . Coding DNA sequences (CDSs) were predicted using Prodigal  and functional annotation was achieved using BLAST+  and HMMER3  against the UniProtKB database .
The genome of Oceanobacillus picturae contained 3,675,175 bp with a G + C content of 39.15 % (Fig. 5, Tables 3 and 4). The genome was shown to encode at least 157 predicted RNA including 14 rRNA, 31 tRNA, 1 tmRNA and 111 miscellaneous RNA. In addition, 3666 genes were identified, representing a coding capacity of 3,125,691 bp (coding percentage: 85.05 %). Among these genes, 269 (7.34 %) were found as putative proteins and 891 (24.3 %) were assigned as hypothetical proteins. Moreover, 3595 genes matched at least one sequence in Clusters of Orthologous Groups database  with BLASTP default parameters. The properties and the statistics of the genome are summarized in Tables 4 and 5. The distribution of genes into COGs functional categories is presented in Table 6 .
Genome comparison with O. picturae with O. kimchii
We performed a brief comparison of Oceanobacillus picturae strain S1 genome sequence against Oceanobacillus kimchii X50 (NZ_CM001792), which is currently the closest available sequenced genome based on rRNA 16S identity. The draft genome sequence of Oceanobacillus picturae has a similar size to the Oceanobacillus kimchii (respectively 3.67 Mb versus 3.83 Mb). The G + C content was higher as compared to Oceanobacillus kimchii (respectively 39.15 and 35.2 %). Oceanobacillus picturae shared an almost identical number of genes (3823 genes versus 3879 genes), with a similar ratio of genes per Mb (1041 genes/Mb versus 1012 genes/Mb). Additional file 1: Table S1 presents the difference in gene number (percentage) related to each COG categories between O. pictuare and O. kimchii . The proportion of COGs is very similar between the two species. The maximum difference is related to the COG “Carbohydrate transport and metabolism” which does not exceed 1.15 %. Additional file 2: Table S2 presents the associated MIGS records.
Collection de souches de l’Unité des Rickettsies
Deutsche Sammlung von Mikroorganismen
Human microbiome project [http://www.hmpdacc.org/catalog/]
Lagier J-C, Hugon P, Khelaifia S, Fournier PE, La Scola B, Raoult D. The rebirth of culture in microbiology through the example of culturomics to study human gut microbiota. Clin Microbiol Rev. 2015;28:237–64.
Ramasamy D, Mishra AK, Lagier JC, Padhmanabhan R, Rossi-Tamisier M, Sentausa E, et al. A polyphasic strategy incorporating genomic data for the taxonomic description of new bacterial species. Int J Syst Evol Microbiol. 2014;64:384–91.
Lu J, Nogi Y, Takami H. Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge. FEMS Microbiol Lett. 2001;205:291–7. PubMed http://dx.doi.org/10.1111/j.1574-6968.2001.tb10963.x.
Yumoto I, Hirota K, Nodasaka Y, Nakajima K. Oceanobacillus oncorhynchi sp. nov., a halotolerant obligate alkaliphile isolated from the skin of a rainbow trout (Oncorhynchus mykiss), and emended description of the genus Oceanobacillus. Int J Syst Evol Microbiol. 2005;55:1521–4. PubMed http://dx.doi.org/10.1099/ijs.0.63483-06.
Heyrman J, Logan NA, Busse HJ, Balcaen A, Lebbe L, Rodriguez-Diaz M, et al. Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus. Int J Syst Evol Microbiol. 2003;53(Pt 2):501–11.
Lee JS, Lim JM, Lee KC, Lee JC, Park YH, Kim CJ. Virgibacillus koreensis sp. nov., a novel bacterium from a salt field, and transfer of Virgibacillus picturae to the genus Oceanobacillus as Oceanobacillus picturae comb. nov. with emended descriptions. Int J Syst Evol Microbiol. 2006;56(Pt 1):251–7.
Raats D, Halpern M. Oceanobacillus chironomi sp. nov., a halotolerant and facultatively alkaliphilic species isolated from a chironomid egg mass. Int J Syst Evol Microbiol. 2007;57:255–9. PubMed http://dx.doi.org/10.1099/ijs.0.64502-0.
Nam JH, Bae W, Lee DH. Oceanobacillus caeni sp. nov., isolated from a Bacillus-dominated wastewater treatment system in Korea. Int J Syst Evol Microbiol. 2008;58:1109–13. PubMed http://dx.doi.org/10.1099/ijs.0.65335-0.
Hirota K, Aino K, Nodasaka Y, Yumoto I. Oceanobacillus indicireducens sp. nov., a facultative alkaliphile that reduces an indigo dye. Int J Syst Evol Microbiol. 2013;63:1437–42. PubMed http://dx.doi.org/10.1099/ijs.0.034579-0.
Kim YG, Choi DH, Hyun S, Cho BC. Oceanobacillus profundus sp. nov., isolated from a deep-sea sediment core. Int J Syst Evol Microbiol. 2007;57:409–13. PubMed http://dx.doi.org/10.1099/ijs.0.64375-0.
Romano I, Lama L, Nicolaus B, Poli A, Gambacorta A, Giordano A. Oceanobacillus oncorhynchi subsp. incaldanensis subsp. nov, an alkalitolerant halophile isolated from an algal mat collected from a sulfurous spring in Campania (Italy), and emended description of Oceanobacillus oncorhynchi. Int J Syst Evol Microbiol. 2006;56:805–10. PubMed http://dx.doi.org/10.1099/ijs.0.63575-0.
Namwong S, Tanasupat S, Lee KC, Lee JS. Oceanobacillus kapialis sp. nov., from fermented shrimp paste in Thailand. Int J Syst Evol Microbiol. 2009;59:2254–9. PubMed http://dx.doi.org/10.1099/ijs.0.007161-0.
Tominaga T, An SY, Oyaizu H, Yokota A. Oceanobacillus sojae sp. nov. isolated from soy sauce production equipment in Japan. Int J Syst Evol Microbiol. 2009;55:225–32. PubMed.
Whon TW, Jung MJ, Roh SW, Nam YD, Park EJ, Shin KS, et al. Oceanobacillus kimchii sp. nov. isolated from a traditional Korean fermented food. J Microbiol. 2010;48:862–6. PubMed http://dx.doi.org/10.1007/s12275-010-0214-7.
Lagier JC, Armougom F, Million M, Hugon P, Pagnier I, Robert C, et al. Microbial culturomics: paradigm shift in the human gut microbiome study. Clin Microbiol Infect. 2012;18(12):1185–93. doi:10.1111/1469-0691.12023. Epub 2012 Oct 3.
Roux V, Million M, Robert C, Magne A, Raoult D. Non-contiguous finished genome sequence and description of Oceanobacillus massiliensis sp. nov. Stand Genomic Sci. 2013;9:370–84. http://dx.doi.org/10.4056/sigs.4267953.
Field D, Garrity G, Gray T, Morrison N, Selengut J, Sterk P, et al. The minimum information about a genome sequence (MIGS) specification. Nat Biotechnol. 2008;26:541–7. PubMed http://dx.doi.org/10.1038/nbt1360.
Woese CR, Kandler O, Wheelis ML. Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya. Proc Natl Acad Sci U S A. 1990;87:4576–9. doi:10.1073/pnas.87.12.4576. pmid:2112744.
Murray RGE. The Higher Taxa, or, a Place for Everything? In: Holt JG, editor. Bergey’s Manual of Systematic Bacteriology, vol. 1. 1st ed. Baltimore: The Williams and Wilkins Co.; 1984. p. 31–4.
Garrity GM, Holt JG. The Road Map to the Manual. In: Garrity GM, Boone DR, Castenholz RW, editors. Bergey’s Manual of Systematic Bacteriology, vol. 1. 2nd ed. New York: Springer; 2001. p. 119–69.
Gibbons NE, Murray RGE. Proposals Concerning the Higher Taxa of Bacteria. Int J Syst Bacteriol. 1978;28:1–6. doi:10.1099/00207713-28-1-1.
Ludwig W, Schleifer KH, Whitman WB. Class I. Bacilli class nov. In: De Vos P, Garrity G, Jones D, Krieg NR, Ludwig W, Rainey FA, Schleifer KH, Whitman WB, editors. Bergey's Manual of Systematic Bacteriology, Second Edition, Volume 3. New York: Springer; 2009. p. 19–20.
Euzéby J. List of new names and new combinations previously effectively, but not validly, published. List no. 132. Int J Syst Evol Microbiol. 2010;60:469–72. doi:10.1099/ijs.0.022855-0.
Skerman VBD, McGowan V, Sneath PHA. Approved Lists of Bacterial Names. Int J Syst Bacteriol. 1980;30:225–420. doi:10.1099/00207713-30-1-225.
Fischer A. Untersuchungen über bakterien. Jahrbücher für Wissenschaftliche Botanik. 1895;27:1–163.
List Editor. Validation List no. 85. Validation of publication of new names and new combinations previously effectively published outside the IJSEM. Int J Syst Evol Microbiol. 2002;52:685–90. doi:10.1099/ijs.0.02358-0.
Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, et al. Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet. 2000;25:25–9. doi:10.1038/75556.. PubMed
Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, Chevenet F, et al. Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res. 2008;36(Web Server issue):W465–9. Epub 2008 Apr 19.
Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 2004;32(5):1792–7.
Castresana J. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol. 2000;17:540–52.
Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O. New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0. Syst Biol. 2010;59(3):307–21.
Lohse M, Bolger AM, Nagel A, Fernie AR, Lunn JE, Stitt M, et al. RobiNA: a user-friendly, integrated software solution for RNA-Seq-based transcriptomics. Nucleic Acids Res. 2012;40(Web Server issue):W622–7.
Nurk S, Bankevich A, Antipov D, Gurevich AA, Korobeynikov A, Lapidus A, et al. Assembling single-cell genomes and mini-metagenomes from chimeric MDA products. J Comput Biol. 2013;20(10):714–37.
Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol. 2012;19(5):455–77.
Boetzer M, Henkel CV, Jansen HJ, Butler D, Pirovano W. Bioinformatics. 2011;27(4):578–9. doi:10.1093/bioinformatics/btq683
Gao S, Sung WK, Nagarajan N. Opera: reconstructing optimal genomic scaffolds with high-throughput paired-end sequences. J Comput Biol. 2011;18(11):1681–91.
Boetzer M, Pirovano W. Toward almost closed genomes with GapFiller. Genome Biol. 2012;13:R56.
Lagesen K, Hallin P, Rodland EA, Staerfeldt HH, Rognes T, Ussery DW. RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res. 2007;35:3100–8.
Laslett D, Canback B. ARAGORN, a program to detect tRNA genes and tmRNA genes in nucleotide sequences. Nucleic Acids Res. 2004;32:11–6.
Griffiths-Jones S, Bateman A, Marshall M, Khanna A, Eddy SR. Rfam: an RNA family database. Nucleic Acids Res. 2003;31:439–41.
Punta M, Coggill PC, Eberhardt RY, Mistry J, Tate J, Boursnell C, et al. The Pfam protein families database. Nucleic Acids Res. 2012;40:D290–301.
Nawrocki EP, Kolbe DL, Eddy SR. Infernal 1.0: inference of RNA alignments. Bioinformatics. 2009;25(10):1335–7.
Hyatt D, Chen GL, Locascio PF, Land ML, Larimer FW, Hauser LJ. Prodigal: prokaryotic gene recognition and translation initiation site identification. BMC Bioinformatics. 2010;11:119.
Camacho C, Coulouris G, Avagyan V, Ma N, Papadopoulos J, Bealer K, et al. BLAST+: architecture and applications. BMC Bioinformatics. 2009;10:421.
Eddy SR. Accelerated profile HMM searches. PLoS Comp Biol. 2011;7(10):e1002195.
The UniProt Consortium. Ongoing and future developments at the UniversalProtein Resource. Nucleic Acids Res. 2011;39:D214–9.
Tatusov RL, Galperin MY, Natale DA, Koonin EV. The COG database: a tool for genomoe-scale analysis of protein functions and evolution. Nucleic Acids Res. 2000;28:33–6.
This project was funded by the Deanship of Scientific Research (DSR), King Abdulaziz University, Jeddah, under grant no. (HiCi-1434-140-30). The authors therefore acknowledge with thanks the DSR technical and financial support.
We thank Karolina Griffiths for English reviewing.
The authors declare that they have no competing interests.
DR conceived the study, JCL, PEF, DR participated in its design and drafted the manuscript, JCL, SK, EIA, OC, FB, AAJF, MY, HBH contributed materials and analyses and helped to draft the manuscript, CR performed the genome sequencing. OC performed the genome analysis. All authors read and approved the final manuscript.
Jean-Christophe Lagier and Saber Khelaifia contributed equally to this work.
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Lagier, J., Khelaifia, S., Azhar, E.I. et al. Genome sequence of Oceanobacillus picturae strain S1, an halophilic bacterium first isolated in human gut. Stand in Genomic Sci 10, 91 (2015). https://doi.org/10.1186/s40793-015-0081-2
- Oceanobacillus picturae
- Halophilic bacteria
- Human gut