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Complete genome sequence of Pseudomonas fluorescens strain PICF7, an indigenous root endophyte from olive (Olea europaea L.) and effective biocontrol agent against Verticillium dahliae
Standards in Genomic Sciences volume 10, Article number: 10 (2015)
Pseudomonas fluorescens strain PICF7 is a native endophyte of olive roots. Previous studies have shown this motile, Gram-negative, non-sporulating bacterium is an effective biocontrol agent against the soil-borne fungus Verticillium dahliae, the causal agent of one of the most devastating diseases for olive (Olea europaea L.) cultivation. Here, we announce and describe the complete genome sequence of Pseudomonas fluorescens strain PICF7 consisting of a circular chromosome of 6,136,735 bp that encodes 5,567 protein-coding genes and 88 RNA-only encoding genes. Genome analysis revealed genes predicting factors such as secretion systems, siderophores, detoxifying compounds or volatile components. Further analysis of the genome sequence of PICF7 will help in gaining insights into biocontrol and endophytism.
Pseudomonas fluorescens PICF7 is a native colonizer of olive (Olea europaea L.) roots and an in vitro antagonist of the soil-borne fungal phytopathogen Verticillium dahliae Kleb. , the causal agent of Verticillium wilts in a large number of plant species . This strain has been demonstrated to be an effective BCA against Verticillium wilt of olive [1, 3], one of the most important biotic constraints for olive cultivation . Moreover, strain PICF7 is able to display an endophytic lifestyle within olive root tissues under different experimental conditions [3, 5, 6] and induces a broad range of defence responses at both local (roots) and systemic (above-ground organs) level, as well as to activate diverse transcription factors known to be involved in systemic defence responses [7, 8]. Accordingly, a recent study has shown the ability of PICF7 to influence the establishment of the pathogen Pseudomonas savastanoi pv. savastanoi in olive stems and to affect the normal development of olive knots , its associated disease .
In this report, we summarize the complete genome sequence and annotation of PICF7. We also describe its genomic properties, highlighting genes encoding plant-associated factors, colonization abilities and well-known bacterial biocontrol traits. The genome sequencing of PICF7 and its comparison with related published genomes will provide a framework for further functional studies of its rhizosphere competence, biocontrol effectiveness and endophytic lifestyle.
Classification and features
P. fluorescens PICF7 is a motile, Gram-negative, non-sporulating rod in the order Pseudomonadales of the class Gammaproteobacteria. Rod-shaped cells are approximately 0.5 μm in width and 2.0-2.5 μm in length (Figure 1 Left and Centre). The strain is moderately fast-growing, forming 2 mm colonies within 2-3 days at 28°C. Colonies formed on King’s B (KB)  agar plates are yellow-green opaque, domed and moderately mucoid with smooth margins (Figure 1 Right).
PICF7 was isolated from the roots of healthy nursery-produced olive plants cv. Picual in Córdoba province (Southern Spain) . It grows in complex media such as LB  or KB, as well as in minimal media such as Standard Succinate Medium (SSM; pH 7.0) . Even though the optimal growth temperature is 28°C, PICF7 can also slightly replicate at 5°C in liquid LB and KB. However, growth at 37°C was not observed in these culturing media after 24 h. The bacterium is an efficient colonizer of the olive rhizosphere  and displays an endophytic lifestyle [3, 5, 6]. It does not cause any deleterious effect on its original host (olive) [1, 5, 9]. Strain PICF7 has natural resistance to kanamycin (50 mg/L) and nalidixic acid (25 mg/L), and it is possible to develop spontaneous rifampicin-resistant mutants .
Genome sequencing and annotation
Genome project history
P. fluorescens strain PICF7 was selected for sequencing due to its ability to exert biocontrol against Verticillium wilt of olive [1, 3] and to develop an endophytic lifestyle within olive root tissues [5, 6]. The genome project is deposited in the Genomes OnLine Database  and the NCBI BioProject database. The finished genome sequence is in GenBank. A summary of the project information is shown in Table 2.
Growth conditions and DNA isolation
P. fluorescens strain PICF7 was grown in 50 ml of LB medium and incubated for 16 h at 28°C. After this period of time, the OD600 of the culture was 1.2. Serial dilutions from this culture and plating on LB plates yielded 2.8 × 108 CFU/mL of a pure bacterial culture (colonies showed uniform morphology and kanamycin resistance). The culture was divided into two 25-ml aliquots and total genomic DNA was extracted using the ‘Jet-Flex genomic DNA purification’ kit (Genomed GmbH, Löhne, Germany), according to the manufacturer’s indications. DNA samples were further purified by extraction with phenol:chloroform and precipitation with ethanol. DNA quality and quantity were checked by agarose gel electrophoresis, spectrophotometry using a ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE), and digestion with different restriction enzymes. Two DNA aliquots (0.6 μg/μL, ~20 μg each) were sent in a dry ice container to the sequencing service.
Genome sequencing and assembly
The genome of PICF7 was sequenced at the Beijing Genomics Institute (BGI) using Solexa paired-end sequencing. Draft assemblies were based on 3,482,351 reads with a length of 500 bp resulting in 1,200 Mb, 2,456,221 reads with a length of 2,000 bp resulting in 1,209 Mb and 1,924,515 reads with a length of 6,000 bp resulting in 1,309 Mb. The SOAPdenovo 1.05 software package [32–34] developed by BGI was used for sequence assembly and quality assessment.
Automatic annotation was performed using the NCBI Prokaryotic Genome Annotation Pipeline. Identification of known type III effectors effectors was conducted by BLASTP searches of the effectors described in http://pseudomonas-syringae.org/ against the proteome of PICF7. Functional annotation was performed by aligning the predicted protein sequences against the COG PSSM of the CDD using RPS-BLAST. Hits with an E-value < = 0.001 were first retained. Then, only the best hit was selected for each protein. Signal peptides and transmembrane helices were predicted using SignalP [35, 36] and TMHMM [37, 38], respectively.
The genome of PICF7 is composed of one circular chromosome of 6,136,735 bp with an average GC content of 60.4% (Table 3 and Figure 3), which is similar to that of other P. fluorescens strains. Among the 5,655 predicted genes, 5,567 were identified as protein coding genes. Of the last, 4,573 (82.1%) were assigned a putative function, while the other 994 (17.9%) were designated as hypothetical proteins. The classification of CDSs into functional categories according to the COG (Clusters of Orthologous Groups) [39, 40] database is summarized in Table 4.
Insights from the genome sequence
The genome contains a complete canonical type III secretion system and two known effector proteins, namely, AvrE1 and HopB1. In addition, two complete type VI secretion system (T6SS) clusters were identified. T6SS has been described to promote antibacterial activity against a wide range of competitor bacteria . PICF7 genome also encodes gene clusters for the synthesis of the siderophores pyochelin and pyoverdine and the hemophore HasAp. A repertoire of cell adhesion proteins has been also identified, including two filamentous hemagglutinin proteins and several fimbrial proteins clustered together with a number of pilus assembly proteins. Notably, two genes have been found to show high similarity with attC and attG genes from Agrobacterium, whose mutation leads to lack of attachment on tomato, carrot, and Bryophyllum daigremontiana .
It is worth mentioning the presence of genome components presumably involved in the synthesis of detoxifying compounds. Such is the case of two clusters containing genes for copper resistance and for production of a cbb(3)-type cytochrome C oxidase, respectively. An ortholog of the gene that codes for Dps, a ferritin-like protein reported to protect plant-associated bacteria against oxidative stress , has also been found. Additional identified traits involved in detoxification are orthologs of catalase KatB and hydroperoxidase KatG, which detoxify plant-produced H2O2 , and a gene coding for a proline iminopeptidase, which has been shown to have dealanylating activity toward the antibiotic ascamycin . A gene predicting a salycilic hydroxylase has been also identified in PICF7 genome. This gene could be involved in the degradation of the plant defence hormone salicylic acid, thus disrupting the systemic response against colonizing bacteria. In addition, all genes required for biosynthesis of the exopolysaccharide alginate  are present in a gene cluster.
Genes predicting volatile components are present in PICF7 genome as well. Volatile components have been shown to act as antibiotics and to induce plant growth [47, 48]. An example is hydrogen cyanide (HCN), an inorganic compound with antagonistic effects against soil microbes . Orthologs of genes required for the biosynthesis of other volatile components such as 2,3-butanediol and acetoin were also found. Further genome analysis revealed other factors presumably involved in the endophytic fitness of PICF7. Such is the case of enzymes like a cellulase and a phytase, as well as the gene coding for aminocyclopropane-1-carboxylate deaminase suggested to be key in the modulation of ethylene levels in plants by bacteria .
In this report we describe the complete genome sequence of Pseudomonas fluorescens strain PICF7, a “Pseudomonadales” in the order Gammaproteobacteria that was originally isolated from the roots of healthy nursery-produced olive plants cv. Picual in Córdoba province, Spain. This strain was selected for sequencing based on its ability to exert biocontrol against Verticillium wilt of olive and to develop an endophytic lifestyle within olive root tissues. Such properties likely have origins in a repertoire of genes including a putative T3SS, two putative T6SS, and several genes presumably implicated in siderophore production. It also has a collection of genes predicting adhesion proteins, detoxifying compounds, volatile components and enzymes such as a cellulase, aphytase and a deaminase. Further functional studies and comparative genomics with related isolates will provide insights into biocontrol and endophytism.
Mercado-Blanco J, Rodríguez-Jurado D, Hervas A, Jiménez-Díaz RM: Suppression of Verticillium wilt in olive planting stocks by root-associated fluorescent Pseudomonas spp. Biol Control 2004, 30:474–86. 10.1016/j.biocontrol.2004.02.002
Pegg GF, Brady BL: Verticillium wilts. Wallingford: CAB International; 2002.
Prieto P, Navarro-Raya C, Valverde-Corredor A, Amyotte SG, Dobinson KF, Mercado-Blanco J: Colonization process of olive tissues by Verticillium dahliae and its in planta interaction with the biocontrol root endophyte Pseudomonas fluorescens PICF7. Microb Biotechnol 2009, 2:499–511. 10.1111/j.1751-7915.2009.00105.x
López-Escudero FJ, Mercado-Blanco J: Verticillium wilt of olive: a case study to implement an integrated strategy to control a soil-borne pathogen. Plant Soil 2011, 344:1–50. 10.1007/s11104-010-0629-2
Prieto P, Mercado-Blanco J: Endophytic colonization of olive roots by the biocontrol strain Pseudomonas fluorescens PICF7. FEMS Microbiol Ecol 2008, 64:297–306. 10.1111/j.1574-6941.2008.00450.x
Prieto P, Schilirò E, Maldonado-González M, Valderrama R, Barroso-Albarracín JB, Mercado-Blanco J: Root hairs play a key role in the endophytic colonization of olive roots by Pseudomonas spp. with biocontrol activity. Microb Ecol 2011, 62:435–45. 10.1007/s00248-011-9827-6
Schilirò E, Ferrara M, Nigro F, Mercado-Blanco J: Genetic responses induced in olive roots upon colonization by the biocontrol endophytic bacterium Pseudomonas fluorescens PICF7. PLoS ONE 2012, 7:e48646. 10.1371/journal.pone.0048646
Gómez-Lama Cabanás C, Schilirò E, Valverde-Corredor A, Mercado-Blanco J: The biocontrol endophytic bacterium Pseudomonas fluorescens PICF7 induces systemic defense responses in aerial tissues upon colonization of olive roots. Front Microbiol 2014, 5:427.
Maldonado-González MM, Prieto P, Ramos C, Mercado-Blanco J: From the root to the stem, interaction between the biocontrol root endophyte Pseudomonas fluorescens PICF7 and the pathogen Pseudomonas savastanoi NCPPB 3335 in olive knots. Microb Biotechnol 2013, 6:275–87. 10.1111/1751-7915.12036
Ramos C, Matas IM, Bardaji L, Aragón IM, Murillo J: Pseudomonas savastanoi pv. savastanoi: some like it knot. Mol Plant Pathol 2012, 13:998–1009. 10.1111/j.1364-3703.2012.00816.x
King EO, Ward M, Raney D: Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med 1954, 44:301–7.
Bertani G: Studies on lysogenesis. I. The mode of phage liberation by lysogenic. J Bacteriol 1951, 62:293–300.
Meyer JM, Abdallah MA: The fluorescent pigment of Pseudomonas fluorescens : biosynthesis, purification and physicochemical properties. J Gen Microbiol 1978, 107:319–28. 10.1099/00221287-107-2-319
Field D, Garrity G, Gray T, Morrison N, Selengut J, Sterk P, Tatusova T, Thomson N, Allen MJ, Angiuoli SV, Ashburner M, Axelrod N, Baldauf S, Ballard S, Boore J, Cochrane G, Cole J, Dawyndt P, Vos PD, dePamphilis C, Edwards R, Faruque N, Feldman R, Gilbert J, Gilna P, Glöckner FO, Goldstein P, Guralnick R, Haft D, Hancock D, et al.: The minimum information about a genome sequence (MIGS) specification. Nat Biotechnol 2008, 26:541–7. 10.1038/nbt1360
Woese CR: Towards a natural system of organisms: Proposal for the domains Archea, Bacteria and Eucarya. Proc Natl Acad Sci USA 1990, 87:4576–9. 10.1073/pnas.87.12.4576
Garrity GM, Bell JA, Phylum LT, XIV: phyl. nov. In Bergey's Manual of Systematic Bacteriol-ogy. Volume 2. 2nd edition. Edited by: Garrity GM, Brenner DJ, Krieg NR, Staley JT. Springer, New York: Part B; 2005:1.
Validation of publication of new names and new combinations previously effectively published outside the IJSEM. List no. 106 Int J Syst Evol Microbiol 2005, 55:2235–8. http://dx.doi.org/10.1099/ijs.0.64108-0
Garrity GM, Bell JA, Class LT, III: class. nov. In Bergey's Manual of Systematic Bacteriology. Volume 2. 2nd edition. Edited by: Garrity GM, Brenner DJ, Krieg NR, Staley JT. Springer, New York: Part B; 2005:1.
Skerman VBD, McGowan V, Sneath PHA: Approved lists of bacterial names. Int J Syst Bacteriol 1980, 30:225–420. http://dx.doi.org/10.1099/00207713-30-1-225 10.1099/00207713-30-1-225
Orla-Jensen S: The main lines of the natural bacterial system. J Bacteriol 1921, 6:263–73.
Garrity G, Bell J, Lilburn T: Bergey’s Manual of Systematic Bacteriology. Volume 2, Part B. 2nd edition. Edited by: Garrity G, Brenner D, Krieg N, Staley J. New York: Springer; 2005:323.
Winslow CEA, Broadhurst J, Buchanan RE, Krumwiede C, Rogers LA, Smith GH: The fami-lies and genera of the bacteria. Preliminary report of the committee of the society of American Bacteriologists on characterization and classification of bacterial types. J Bacteriol 1917, 2:505–66.
Migula W: Über ein neues System der Bakterien. Arb Bakteriol Inst Karlsruhe 1894, 1:235–8.
Doudoroff M, Palleroni NJ: Genus Migula 1894, 237; Nom. cons. Opin. 5, Jud. Comm. 1952, 121. In Bergey's Manual of Determinative Bacteriology. 8th edition. Edited by: Buchanan RE, Gibbons NE. Baltimore: The Williams and Wilkins Co.; 1974:217–43.
Commission J: Opinion 5: Conservation of the generic name Migula 1894 and designation of aeruginosa (Schroeter) Migula 1900 as type species. Int Bull Bacteriol Nomencl Taxon 1952, 2:121–2.
Palleroni NJ: Pseudomonadaceae. Bergey's Manual of Systematic Bacteriology. Edited by: Krieg NR, Holt JG. Baltimore: The Williams and Wilkins Co; 1984:141–99.
Anzai Y, Kim H, Park JY, Wakabayashi H, Oyaizu H: Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Intl J Sys Evol Microbiol 2000, 50:1563–89. 10.1099/00207713-50-4-1563
Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, Harris MA, Hill DP, Issel-Tarver L, Kasarskis A, Lewis S, Matese JC, Richardson JE, Ringwald M, Rubin GM, Sherlock G: Gene Ontology: tool for the unification of biology. Nat Genet 2000, 25:25–9. 10.1038/75556
Jones DT, Taylor WR, Thornton JM: The rapid generation of mutation data matrices from protein sequences. Comput Appl Biosci 1992, 8:275–82.
Felsenstein J: Confidence limits on phylogenies – an approach using the bootstrap. Evolution 1985, 39:783–91. 10.2307/2408678
Liolios K, Mavromatis K, Tavernarakis N, Kyrpides NC: The Genomes On Line Database (GOLD) in 2007: status of genomic and metagenomic projects and their associated metadata. Nucleic Acids Res 2008, 36:D475–9. 10.1093/nar/gkn240
Li R, Zhu H, Ruan J, Qian W, Fang X, Shi Z, Li Y, Li S, Shan G, Kristiansen K, Li S, Yang H, Wang J, Wang J: De novo assembly of human genomes with massively parallel short read sequencing. Genome Res 2010, 20:265–272. 10.1101/gr.097261.109
Li R, Li Y, Kristiansen K, Wang J: SOAP: short oligonucleotide alignment program. Bioinformatics 2008, 24:713. 10.1093/bioinformatics/btn025
SOAP. denovo v.1.05. http://soap.genomics.org.cn/soapdenovo.html
Signal P. http://www.cbs.dtu.dk/services/SignalP/
Emanuelsson O, Brunak S, von Heijne G, Nielsen H: Locating proteins in the cell using TargetP, SignalP and related tools. Nat Protoc 2007, 2:953–71. 10.1038/nprot.2007.131
Krogh A, Larsson B, von Heijne G, Sonnhammer EL: Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol 2001, 305:567–80. 10.1006/jmbi.2000.4315
TMHMM. Transmembrane domain prediction. http://www.cbs.dtu.dk/services/TMHMM/
Tatusov RL, Koonin EV, Lipman DJ: A genomic perspective on protein families. Science 1997, 278:631–7. 10.1126/science.278.5338.631
Clusters of Ortholog ous Groups. http://www.ncbi.nlm.nih.gov/COG
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD: A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria. Cell Host Microbe 2010, 7:25–37. 10.1016/j.chom.2009.12.007
Matthysse AG, Jaeckel P, Jeter C: attG and attC mutations of Agrobacterium tumefaciens are dominant negative mutations that block attachment and virulence. Can J Microbiol 2008, 54:241–7. 10.1139/W08-005
Colburn-Clifford JM, Scherf JM, Allen C: Ralstonia solanacearum Dps contributes to oxidative stress tolerance and to colonization of and virulence on tomato plants. Appl Environ Microbiol 2010, 76:7392–9. 10.1128/AEM.01742-10
Guo M, Block A, Bryan CD, Becker DF, Alfano JR: Pseudomonas syringae Catalases Are Collectively Required for Plant Pathogenesis. J Bacteriol 2012, 194:5054–64. 10.1128/JB.00999-12
Sudo T, Shinohara K, Dohmae N, Takio K, Usami R, Horikoshi K, Osada H: Isolation and characterization of the gene encoding an aminopeptidase involved in the selective toxicity of ascamycin toward Xanthomonas campestris pv. citri. Biochem J 1996,319(Pt 1):99–102.
Peñaloza-Vázquez A, Kidambi SP, Chakrabarty AM, Bender CL: Characterization of the alginate biosynthetic gene cluster in Pseudomonas syringae pv. syringae. J Bacteriol 1997, 179:4464–72.
Ren Y, Strobel G, Sears J, Park M: Geobacillus sp., a thermophilic soil bacterium producing volatile antibiotics. Microb Ecol 2010, 60:130–6. 10.1007/s00248-009-9630-9
Ryu CM, Farag MA, Hu CH, Reddy MS, Wei HX, Paré PW, Kloepper JW: Bacterial volatiles promote growth in Arabidopsis . Proc Natl Acad Sci U S A 2003, 100:4927–32. 10.1073/pnas.0730845100
Ahmad F, Ahmad I, Khan MS: Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol Res 2008, 163:173–81. 10.1016/j.micres.2006.04.001
Hardoim PR, van Overbeek LS, van Elsas JD: Properties of bacterial endophytes and their proposed role in plant growth. Trends Microbiol 2008, 16:463–71. 10.1016/j.tim.2008.07.008
This Project was supported by grants P07-CVI-02624, P10-AGR-5797, P12-AGR-667 (Convocatoria Proyectos de Excelencia from Junta de Andalucía, Spain), co-funded by ERDF from EU, grant AGL2011-30343-CO2-01 from the MINECO (Spain), co-financed by FEDER, and the Campus de Excelencia Internacional Andalucía Tech. P. M. Martínez was supported by the Campus de Exelencia Internacional Andalucía Tech. The authors would like to thank Prof. Antonio de Vicente and Dr. Eva Arrebola for the logistics and shipment of DNA samples to BGI.
The authors declare that they have no competing interests.
JMB conceived and designed the experiments. DRR, ES and PP performed the experiments. PMMG performed the annotation and sequence homology searches. PMMG, CR, JMB and PRP wrote the manuscript. All authors commented on the manuscript before submission. All authors read and approved the final manuscript.
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Martínez-García, P.M., Ruano-Rosa, D., Schilirò, E. et al. Complete genome sequence of Pseudomonas fluorescens strain PICF7, an indigenous root endophyte from olive (Olea europaea L.) and effective biocontrol agent against Verticillium dahliae . Stand in Genomic Sci 10, 10 (2015). https://doi.org/10.1186/1944-3277-10-10
- Pseudomonas fluorescens
- Verticillium wilt
- Detoxification systems