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Non-contiguous finished genome sequence and description of Fenollaria massiliensis gen. nov., sp. nov., a new genus of anaerobic bacterium
Standards in Genomic Sciences volume 9, pages704–717(2014)
Fenollaria massiliensis strain 9401234T, is the type strain of Fenollaria massiliensis gen. nov., sp. nov., a new species within a new genus Fenollaria. This strain, whose genome is described here, was isolated from an osteoarticular sample. F. massiliensis strain 9401234T is an obligate anaerobic Gram-negative bacillus. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 1.71 Mbp long genome exhibits a G+C content of 34.46% and contains 1,667 protein-coding and 30 RNA genes, including 3 rRNA genes.
Fenollaria massiliensis strain 9401234T (= CSUR P127 = DSM 26367), is the type strain of Fenollaria massiliensis sp. nov., and the first member of the new genus Fenollaria gen. nov. This bacterium is a Gram-negative, anaerobic, non spore-forming, indole positive bacillus that was isolated from an osteoarticular sample, during a study prospecting anaerobic isolates from deep samples .
Traditionally, definition of a new bacterial species or genus has relied on the application of the “gold standard” methods of DNA-DNA hybridization and G+C content determination . However, those methods are expensive, and poorly reproducible. The development of PCR and sequencing methods led to new ways of classifying bacterial species, using, in particular, 16S rRNA sequences with cutoff , together with phenotypic characteristics. Recently, a number of new bacterial genera and species have been described using high throughput genome sequencing and mass spectrometric analyses, which allows access to a wealth of genetic and proteomic information [4,5]. We propose a new bacterial genus and species using a whole genome sequence and a MALDI-TOF spectrum, and the main characteristics of the organism, as we have previously done [6–12].
Here we present a summary classification and a set of features for F. massiliensis gen. nov., sp. nov. strain 9401234T (= CSUR P127= DSM 26367) together with the description of the complete genomic sequencing and annotation. These characteristics support the circumscription of a novel genus, Fenollaria gen. nov., within the Clostridiales Family XI Incertae sedis, with Fenollaria massiliensis gen. nov., sp. nov, as the type species.
Clostridiales Family XI Incertae sedis was created in 2009 , and currently comprises 11 genera, including Anaerococcus, Peptoniphilus and Tissierella. It is a heterogeneous group that includes anaerobic and morphologically variable bacteria. This group is defined mainly on the basis of phylogenetic analyses of 16S rRNA sequences and its members have no precise taxonomic or phylogenetic affiliation. Based on the 16S rRNA comparison, the species most closely related to Fenollaria massiliensis is Sporobacterium olearium , which is the sole representative of the genus Sporobacterium. S. olearium is a Gram-positive rod with terminal spores. The most closely related validly named species is Tissierella creatinini, which belongs to the genus Tissierella sp . It was first described in 1986 and is represented by three species, among which the type species is T. praecuta, a strictly anaerobic Gram-negative, non spore-forming bacterium.
Classification and features
An osteoarticular sample was collected from a patient as part of a study analyzing emerging anaerobic infectious agents by MALDI-TOF and 16S rRNA gene sequencing. The specimen was sampled in Marseille and preserved at −80°C after collection. Strain 9401234T (Table 1) was isolated in February 2009, by anaerobic cultivation on 5% sheep blood-enriched Columbia agar (BioMerieux, Marcy l’Etoile, France). Based on the 16S rRNA sequencing, this strain exhibited 87% sequence similarity with Tissierella creatinini . In the inferred phylogenetic tree, it forms a distinct lineage within the Clostridiales Family XI Incertae sedis (Figure 1). Those similarity values are lower than the recommended threshold to delineate a new genus without carrying out DNA-DNA hybridization .
Growth at different temperatures was tested; no growth occurred at 23°C, 25°C, 28°C and 50°C, but did occur between 32° and 37°C. Optimal growth was observed at 37°C.
Colonies are punctiform, grey, smooth, and round when grown on blood-enriched Columbia agar (Biomerieux), under anaerobic conditions using GENbag anaer (BioMérieux). Growth was achieved anaerobically, on blood-enriched Columbia agar and in TS broth medium after 72h. They also were grown under anaerobic conditions on BHI agar supplemented with 1% NaCl. Growth did not occur under microaerophilic conditions and in the presence of air, with 5% CO2.. Gram staining showed rod-shaped non spore-forming Gram-negative bacilli (Figure 2). Cells were non-motile. Cells grown in TS broth medium have a mean length of 1.555 µm (min = 1.167µm; max = 2.948µm), and a mean width of 0.772 µm (min = 0.602 µm; max = 1.014 µm), as determined using electron microscopic observation after negative staining (Figure 3).
Strain 9401234T exhibited neither catalase nor oxidase activities. Using the API 20A system, a positive reaction was observed only for indole, and weakly for gelatinase. Using the API Zym system, a positive reaction was observed for leucine arylamidase and valine arylamidase regarding the proteases, and for Naphtol phosphatase. API RapidID 32A confirmed the positivity for indole and leucine arylamidase, and was also positive for arginine arylamidase, and weakly positive for pyrrolidonyl arylamidase, tyrosine arylamidase, glycine arylamidase, histidine arylamidase and serine arylamidase. Regarding antibiotic susceptibility, F. massiliensis was susceptible to penicillin G, amoxicillin, cefotetan, imipenem, metronidazole, and vancomycin. When compared to the species Tissierela creatinini, Sporobacterium olearium, and Anaerococcus prevotii, within the Clostridiales Family XI Incertae sedis, F. massiliensis exhibits the phenotypic characteristics details in Table 2.
Matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) MS protein analysis was carried out as previously described . A pipette tip was used to pick one isolated bacterial colony from a culture agar plate, and to spread it as a thin film on a MTP 384 MALDI-TOF target plate (Bruker Daltonik GmbH, Germany). Ten distinct deposits were done for strain JC122T from ten isolated colonies. Each smear was overlaid with 2µL of matrix solution (saturated solution of alpha-cyano-4-hydroxycinnamic acid) in 50% acetonitrile, 2.5% tri-fluoracetic acid, and allowed to dry for five minutes. Measurements were performed with a Microflex spectrometer (Bruker). Spectra were recorded in the positive linear mode for the mass range of 2,000 to 20,000 Da (parameter settings: ion source 1 (ISI), 20kV; IS2, 18.5 kV; lens, 7 kV). A spectrum was obtained after 675 shots at a variable laser power. The time of acquisition was between 30 seconds and 1 minute per spot. The ten 9401234T spectra were imported into the MALDI Biotyper software (version 2.0, Bruker) and analyzed by standard pattern matching (with default parameter settings) against the main spectra of 5,697 bacteria in the Biotyper database. The method of identification includes the m/z from 3,000 to 15,000 Da. For every spectrum, 100 peaks at most were taken into account and compared with the spectra in database. The output score enabled the identification of the tested species: a score ≥ 2 with a validated species enabled the identification at the species level; a score ≥ 1.7 but < 2 enabled the identification at the genus level; a score < 1.7 was not significant. For strain 9401234T, the obtained score was 1.04, which is not significant, suggesting that our isolate was not a member of a known genus. We added the spectrum from strain 9401234T (Figure 4) to our database. A dendrogram was constructed with the MALDI Biotyper software, comparing the reference spectrum of strain 9401234T with reference spectra of 29 bacterial species, all belonging to the order of Clostridiales (Figure 5). In this dendrogram, strain 9401234T appears in a separate clade between the genus Peptoniphilus and Acidaminococcus (Figure 5).
Genome sequencing and annotation
Genome project history
The organism was selected for sequencing on the basis of its phylogenetic position, 16S rRNA similarity to other members of the Clostridiales Family XI Incertae sedis, and its isolation from an osteoarticular clinical sample. It is the first genome of the new genus Fenollaria (Genbank accession numbers are CALI02000001-CALI02000010) and consists of 11 contigs. Table 3 shows the project information and its association with MIGS version 2.0 compliance.
Growth conditions and DNA isolation
F. massiliensis sp. nov., gen. nov. strain 9401234T, CSUR P127 = DSM 26367, was grown on blood agar medium at 37°C under anaerobic conditions. Ten petri dishes were spread and resuspended in 5×100µl of G2 buffer (EZ1 DNA Tissue kit, Qiagen). A first mechanical lysis was performed by glass powder on the Fastprep-24 device (Sample Preparation system) from MP Biomedicals, USA) using 2×20 seconds cycles. DNA was then treated with 2.5 µg/µL lysozyme (30 minutes at 37°C) and extracted through the BioRobot EZ 1 Advanced XL (Qiagen). The DNA was then concentrated and purified on a Qiamp kit (Qiagen). The yield and the concentration were measured by the Quant-it Picogreen kit (Invitrogen) on the Genios_Tecan fluorometer at 135 ng/µl.
Genome sequencing and assembly
This project was loaded twice on a one-quarter region for the paired end application on PTP Picotiter plates. DNA (5µg) was mechanically fragmented on a Hydroshear device (Digilab, Holliston, MA, USA) with an enrichment size at 3–4kb. The DNA fragmentation was visualized through an Agilent 2100 BioAnalyzer on a DNA LabChip 7500 with an optimal size of 4.2 kb. The library was constructed according to the 454_Titanium paired end protocol and manufacturer recommendations. Circularization and nebulization were performed and generated a pattern with an maximum at 686 bp. After PCR amplification through 15 cycles followed by double size selection, the single stranded paired end library was then quantified on the Agilent 2100 BioAnalyzer with a RNA 6000 Pico chip at 1,820 pg/µL. The library concentration equivalence was calculated as 4.87E+09 molecules/µL. The library was stored at −20°C.
The paired end library was clonal amplified with 1cpb in 3 emPCR reactions with the GS Titanium SV emPCR Kit (Lib-L) v2. The yield of the emPCR was 10.5% according to the quality expected by the range of 5 to 20% from the Roche procedure. 790,000 beads were loaded on the GS Titanium PicoTiterPlates PTP Kit 70×75 sequenced with the GS Titanium Sequencing Kit XLR70. The run was performed overnight and then analyzed on the cluster through the gsRunBrowser and gsAssembler_Roche.
The 454 sequencing generated 119,791 reads (38,34 Mb) and was assembled into contigs and scaffolds using Newbler version 2.6 (Roche) and SSPACE software v1.0  combined with GapFiller V1.10 . A sequence consisting of 6,257,638 reads generated from a SOLiD version 4 with a library constructed through an insert size of 150 bp and a 85 bp (50bp and 35bp) in a paired-end sequencing (Life Technologies) helped to improve the genome assembly using CLC Genomics Workbench v4.7.2 (CLC bio, Aarhus, Denmark). Finally, the available genome consists of 8 scaffolds and 11 contigs.
Non-coding genes and miscellaneous features were predicted using RNAmmer , ARAGORN , Rfam  and signalP . Open Reading Frames (ORFs) were predicted using Prodigal  with default parameters but the predicted ORFs were excluded if they were spanning a sequencing GAP region. The functional annotation was achieved using BLASTP  against the GenBank database  and the Clusters of Orthologous Groups (COG) database.
The genome of Fenollaria massiliensis sp. nov. strain 9401234T is estimated at 1.71 Mb long with a G+C content of 36.47% (Figure 6 and Table 4). A total of 1,667 protein-coding and 30 RNA genes, including 3 rRNA genes, 26 tRNA and 1 tmRNA were found. The majority of the protein-coding genes (70.8%) were assigned a putative function while the remaining ones were annotated as hypothetical proteins. The properties and the statistics of the genome are summarized in Table 4 and Table 5.
Insights into the genome sequence
There is a lack of closely related genomes because Fenollaria gen. nov. is a new genus. However, we made some comparisons against Peptoniphilus sp. oral taxon 386 str. F0131 (accession number NZ_GL349422), which is relatively close to Fenollaria based on 16S rRNA and for which the completed genome is available in public databases.
The draft genome sequence of F. massiliensis has a slightly bigger size compared to the Peptoniphilus sp.(1.71 Mbp and 1.47 Mbp, respectively). The G+C content is slightly higher than Peptoniphilus sp. (34 and 31%, respectively). Fenollaria massiliensis gen. nov. encodes more genes (1,697 genes against 1,463 genes), however the number of genes per Mb is similar (1,007 – 1,004).
Table 6 presents the difference of gene number (in percentage) for each COG categories between Peptoniphilus sp. oral taxon 386 str. F0131 and Fenollaria massiliensis sp. nov.
On the basis of phenotypic, phylogenetic and genomic analyses, we formally propose the creation of Fenollaria massiliensis gen. nov., sp. nov. that contains the strain 9401234T. This bacterium was found in Marseille, France.
Description of Fenollaria gen. nov.
Fenollaria (Fe.nol.la’ria. N.L. gen. n. Fenollaria of F. Fenollar, expert microbiologist in Whipple’s disease and osteo-articular infections)
Gram negative rods. Obligate anaerobic. Non motile, non spore forming. Positive for indole. Negative for catalase and oxidase. Weakly positive gelatinase. Positive for leucine arylamidase, valine arylamidase, arginine arylamidase and for Naphtol phosphatase. Weakly positive for pyrrolidonyl arylamidase, tyrosine arylamidase, glycine arylamidase, histidine arylamidase and serine arylamidase. Habitat: human. Type species: Fenollaria massiliensis
Description of Fenollaria massiliensis gen. nov. sp.nov.
Fenollaria massiliensis (ma.si.li.en’.sis. L. fem. adj. massiliensis, of Massilia, the Latin name of Marseille where was isolated F. massiliensis).
Gram negative, catalase negative, oxidase negative and obligate anaerobic. Cells are non-spore forming, non motile rods, with a mean length of 1,555 µm, and a mean width of 772 µm. Colonies are punctiform, very small, grey, smooth, and round on blood-enriched Columbia agar under anaerobic conditions. Optimal growth under anaerobic conditions, at 37°C (range from 32°C to 37°C). Cells are positive for leucine arylamidase, valine arylamidase, arginine arylamidase and for Naphtol phosphatase. Cells are weakly positive for pyrrolidonyl arylamidase, tyrosine arylamidase, glycine arylamidase, histidine arylamidase and serine arylamidase. Susceptible to penicillin G, amoxicillin, cefotetan, imipenem, metronidazole and vancomycin. The potential pathogenicity of the type strain 9401234T is unknown.
The type strain is 9401234T (= CSUR P127 = DSM 26367); it was isolated from an osteoarticular sample of a patient in Marseille (France). The G+C content of the genome is 34.46 mol%. A partial 16S rRNA gene sequence was deposited in GenBank with the accession number HM587321. The whole genome shotgun sequence of F. massiliensis strain 9401234T (= CSUR P127 = DSM 26367) has been deposited in GenBank under accession numbers CALI02000001-CALI02000010.
La Scola B, Fournier PE, Raoult D. Burden of emerging anaerobes in the MALDI-TOF and 16S rRNA gene sequencing era. Anaerobe 2011; 17:106–112. PubMed http://dx.doi.org/10.1016/j.anaerobe.2011.05.010
Rossello-Mora R. DNA-DNA Reassociation Methods Applied to Microbial Taxonomy and Their Critical Evaluation. In: Stackebrandt E (ed), Molecular Identification, Systematics, and population Structure of Prokaryotes. Springer, Berlin, 2006, p. 23–50.
Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006; 33:152–155.
Welker M, Moore ER. Applications of whole-cell matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry in systematic microbiology. Syst Appl Microbiol 2011; 34:2–11. PubMed http://dx.doi.org/10.1016/j.syapm.2010.11.013
Tindall BJ, Rosselló-Móra R, Busse HJ, Ludwig W, Kämpfer P. Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 2010; 60:249–266. PubMed http://dx.doi.org/10.1099/ijs.0.016949-0
Kokcha S, Michra AK, Lagier JC, Million M, Leroy Q, Raoult D, Fournier PE. Non-contiguous-finished genome sequence and description of Bacillus timonensis sp. nov. Stand Genomic Sci 2012; 6:346–355. PubMed http://dx.doi.org/10.4056/sigs.2776064
Lagier JC, El Karkouri K, Nguyen TT, Armougom F, Raoult D, Fournier PE. Non-contiguous-finished genome sequence and description of Anaerococcus senegalensis sp. nov. Stand Genomic Sci 2012; 6:116–125. PubMed http://dx.doi.org/10.4056/sigs.2415480
Mishra AK, Gimenez G, Lagier JC, Robert C, Raoult D, Fournier PE. Non-contiguous-finished genome sequence and description of Alistipes senegalensis sp. nov. Stand Genomic Sci 2012; 6:304–314. http://dx.doi.org/10.4056/sigs.2625821
Lagier JC, Armougom F, Mishra AK, Nguyen TT, Raoult D, Fournier PE. Non-contiguous-finished genome sequence and description of Alistipes timonensis sp. nov. Stand Genomic Sci 2012; 6:315–324. PubMed http://dx.doi.org/10.4056/sigs.2685971
Mishra AK, Lagier JC, Robert C, Raoult D, Fournier PE. Non-contiguous-finished genome sequence and description of Clostridium senegalense sp. nov. Stand Genomic Sci 2012; 6:386–395. PubMed
Michra AK, Lagier JC, Robert C, Raoult D, Fournier PE. Non-contiguous-finished genome sequence and description of Peptinophilus timonensis sp. nov. Stand Genomic Sci 2012; (In press).
Michra AK, Lagier JC, Robert C, Raoult D, Fournier PE. Non-contiguous-finished genome sequence and description of Peptinophilus senegalensis sp. nov. Stand Genomic Sci 2012; (In press).
Ludwig W, Schleifer KH, Whitman WB. Revised road map to the phylum Firmicutes. In: Bergey’s Manual of Systematic Bacteriology, 2nd ed., vol. 3 (The Firmicutes) (P. De Vos, G. Garrity, D. Jones, N.R. Krieg, W. Ludwig, F.A. Rainey, K.-H. Schleifer, and W.B. Whitman, eds.), Springer-Verlag, New York. (2009) pp. 1–13.
Mechichi T, Labat M, Garcia JL, Thomas P, Patel BK. Sporobacterium olearium gen. nov., sp. nov., a new methanethiol-producing bacterium that degrades aromatic compounds, isolated from an olive mill wastewater treatment digester. Int J Syst Bacteriol 1999; 49:1741–1748. PubMed http://dx.doi.org/10.1099/00207713-49-4-1741
Collins MD, Shah HN. Reclassification of Bacteroides praeacutus Tissier (Holdeman and Moore) in a new genus, Tissierella, as Tissierella praeacuta comb. nov. Int J Syst Bacteriol 1986; 36:461–463. http://dx.doi.org/10.1099/00207713-36-3-461
Field D, Garrity G, Gray T, Morrison N, Selengut J, Sterk P, Tatusova T, Thomson N, Allen MJ, Angiuoli SV, et al. The minimum information about a genome sequence (MIGS) specification. Nat Biotechnol 2008; 26:541–547. PubMed http://dx.doi.org/10.1038/nbt1360
Woese CR, Kandler O, Wheelis ML. Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya. Proc Natl Acad Sci USA 1990; 87:4576–4579. PubMed http://dx.doi.org/10.1073/pnas.87.12.4576
Gibbons NE, Murray RGE. Proposals Concerning the Higher Taxa of Bacteria. Int J Syst Bacteriol 1978; 28:1–6. http://dx.doi.org/10.1099/00207713-28-1-1
Garrity GM, Holt JG. The Road Map to the Manual. In: Garrity GM, Boone DR, Castenholz RW (eds), Bergey’s Manual of Systematic Bacteriology, Second Edition, Volume 1, Springer, New York, 2001, p. 119–169.
Murray RGE. The Higher Taxa, or, a Place for Everything…? In: Holt JG (ed), Bergey’s Manual of Systematic Bacteriology, First Edition, Volume 1, The Williams and Wilkins Co., Baltimore, 1984, p. 31–34.
List of new names and new combinations previously effectively, but not validly, published. List no. 132. Int J Syst Evol Microbiol 2010; 60:469–472. http://dx.doi.org/10.1099/ijs.0.022855-0
Rainey FA. Class II. Clostridia class nov. In: De Vos P, Garrity G, Jones D, Krieg NR, Ludwig W, Rainey FA, Schleifer KH, Whitman WB (eds), Bergey’s Manual of Systematic Bacteriology, Second Edition, Volume 3, Springer-Verlag, New York, 2009, p. 736.
Skerman VBD, Sneath PHA. Approved list of bacterial names. Int J Syst Bact 1980; 30:225–420. http://dx.doi.org/10.1099/00207713-30-1-225
Prevot AR. Dictionnaire des bactéries pathogens. In: Hauduroy P, Ehringer G, Guillot G, Magrou J, Prevot AR, Rosset, Urbain A (eds). Paris, Masson, 1953, p. 1–692.
Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, et al. Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet 2000; 25:25–29. PubMed http://dx.doi.org/10.1038/75556
Farrow JAE, Lawson PA, Hippe H, Gauglitz U, Collins MD. Phylogenetic evidence that the Gram-negative nonsporulating bacterium Tissierella (Bacteroides) preacuta is a member of the Clostridium subphylum of the Gram-positive bacteria, and description of Tisiierella creatinini sp. nov. Int J Syst Bacteriol 1995; 45:436–440. PubMed http://dx.doi.org/10.1099/00207713-45-3-436
Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 2007; 24:1596–1599. PubMed http://dx.doi.org/10.1093/molbev/msm092
Murdoch DA. Gram-positive anaerobic cocci. Clin Microbiol Rev 1998; 11:81–120. PubMed
Seng P, Drancourt M, Gouriet F, La Scola B, Fournier PE, Rolain JM, Raoult D. Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis 2009; 49:543–551. PubMed http://dx.doi.org/10.1086/600885
Boetzer M, Henkel CV, Jansen HJ, Butler D, Pirovano W. Scaffolding pre-assembled contigs using SSPACE. Bioinformatics 2011; 27:578–579. PubMed http://dx.doi.org/10.1093/bioinformatics/btq683
Boetzer M, Pirovano W. Toward almost closed genomes with GapFiller. Genome Biol 2012; 13:R56. PubMed http://dx.doi.org/10.1186/gb-2012-13-6-r56
Lagesen K, Hallin P, Rodland EA, Staerfeldt HH, Rognes T, Ussery DW. RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res 2007; 35:3100–3108. PubMed http://dx.doi.org/10.1093/nar/gkm160
Laslett D, Canback B. ARAGORN, a program to detect tRNA genes and tmRNA genes in nucleotide sequences. Nucleic Acids Res 2004; 32:11–16. PubMed http://dx.doi.org/10.1093/nar/gkh152
Griffiths-Jones S, Bateman A, Marshall M, Khanna A, Eddy SR. Rfam: an RNA family database. Nucleic Acids Res 2003; 31:439–441. PubMed http://dx.doi.org/10.1093/nar/gkg006
Bendtsen JD, Nielsen H, von Heijne G, Brunak S. Improved prediction of signal peptides: SignalP 3.0. J Mol Biol 2004; 340:783–795. PubMed http://dx.doi.org/10.1016/j.jmb.2004.05.028
Hyatt D, Chen GL, Locascio PF, Land ML, Larimer FW, Hauser LJ. Prodigal: prokaryotic gene recognition and translation initiation site identification. BMC Bioinformatics 2010; 11:119. PubMed http://dx.doi.org/10.1186/1471-2105-11-119
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990; 215:403–410. PubMed
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Pagnier, I., Croce, O., Robert, C. et al. Non-contiguous finished genome sequence and description of Fenollaria massiliensis gen. nov., sp. nov., a new genus of anaerobic bacterium. Stand in Genomic Sci 9, 704–717 (2014). https://doi.org/10.4056/sigs.3957647
- Fenollaria massiliensis