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Complete genome sequence of the filamentous gliding predatory bacterium Herpetosiphon aurantiacus type strain (114-95T)
Standards in Genomic Sciences volume 5, pages356–370 (2011)
Herpetosiphon aurantiacus Holt and Lewin 1968 is the type species of the genus Herpetosiphon, which in turn is the type genus of the family Herpetosiphonaceae, type family of the order Herpetosiphonales in the phylum Chloroflexi. H. aurantiacus cells are organized in filaments which can rapidly glide. The species is of interest not only because of its rather isolated position in the tree of life, but also because Herpetosiphon ssp. were identified as predators capable of facultative predation by a wolf pack strategy and of degrading the prey organisms by excreted hydrolytic enzymes. The genome of H. aurantiacus strain 114-95T is the first completely sequenced genome of a member of the family Herpetosiphonaceae. The 6,346,587 bp long chromosome and the two 339,639 bp and 99,204 bp long plasmids with a total of 5,577 protein-coding and 77 RNA genes was sequenced as part of the DOE Joint Genome Institute Program DOEM 2005.
Strain 114-95T (= ATCC 23779 = DSM 785 = CCUG 48726) is the type strain of Herpetosiphon aurantiacus, which in turn is the type species of the genus Herpetosiphon [1,2]. Because most of the species were reclassified as members of other genera in 1998 , only one other species currently remains in this genus: H. geysericola (Copeland 1936) Lewin 1970. The genus name, meaning gliding tube, was derived from the Greek words herpeton, gliding animal or reptile, and siphon, tube or pipe . The species epithet is derived from the Neo-Latin adjective aurantiacus, meaning orange-colored . Strain 114-95T was originally isolated from the slimy coating of a freshwater alga (Chara sp.) in Birch Lake, Minnesota (USA), but strains belonging to the species were also isolated from well water, cow dung, hot springs and marine shores . H. aurantiacus 114-95T is capable of predation of other bacteria and can thereby destroy whole colonies . It has even been suggested that Herpetosiphon spp. are capable of facultative predation by a wolf pack strategy, in which a quorum of predatory cells is required to degrade the prey organism by excreted hydrolytic enzymes . Here we present a summary classification and a set of features for H. aurantiacus 114-95T, together with the description of the complete genome and its annotation.
Classification and features
A representative genomic 16S rRNA sequence of H. aurantiacus strain 114-95T was compared using NCBI BLAST [7,8] under default settings (e.g., considering only the high-scoring segment pairs (HSPs) from the best 250 hits) with the most recent release of the Greengenes database  and the relative frequencies of taxa and keywords (reduced to their stem ) were determined, weighted by BLAST scores. The most frequently occurring genera were Herpetosiphon (82.9%), Chloroflexus (9.9%), ‘Kouleothrix’ (4.5%), Oscillochloris (2.2%) and ‘Chlorothrix’ (0.5%) (46 hits in total). Regarding the 13 hits to sequences from members of the species, the average identity within HSPs was 99.8%, whereas the average coverage by HSPs was 96.9%. Regarding the two hits to sequences from other members of the genus, the average identity within HSPs was 97.8%, whereas the average coverage by HSPs was 94.2%. Among all other species, the one yielding the highest score was Herpetosiphon geysericola (NR_028694), which corresponded to an identity of 97.8% and an HSP coverage of 94.2%. (Note that the Greengenes database uses the INSDC (= EMBL/NCBI/DDBJ) annotation, which is not an authoritative source for nomenclature or classification.) The highest-scoring environmental sequence was JF098937 (‘skin popliteal fossa clone ncd1008d03c1’), which showed an identity of 98.2% and an HSP coverage of 88.3%. The most frequently occurring keywords within the labels of environmental samples that yielded hits were ‘soil’ (4.2%), ‘microbi’ (4.1%), ‘geyser’ (3.3%), ‘geotherm’ (2.9%) and ‘mat’ (2.8%) (204 hits in total). Environmental samples that yielded hits of a higher score than the highest scoring species were not found. These keywords fit well with the ecological properties reported for strain 114-95T in the original description .
Figure 1 shows the phylogenetic neighborhood of H. aurantiacus in a tree based upon 16S rRNA. The sequences of the five 16S rRNA gene copies in the genome differ from each other by up to two nucleotides, and differ by up to seven nucleotides from the previously published 16S rRNA sequence (M34117), which contains 64 ambiguous base calls.
Cells of H. aurantiacus strain 114-95T are cylindrical measuring 1–1.5 µm by 5–10 µm (Figure 2) . Cells are organized in sheathed filaments of 500 µm length or more . However, the existence of a sheath in the classical sense has been questioned in an analysis of the fine structure of the cells . Cells of strain 114-95T stain Gram-negative, are not flagellated but are motile via gliding and divide by the formation of a transverse septum . Colonies are flat, spreading, and rough, and produce an orange pigment . Pigment analyses of a related strain, H. giganteus Hp a2, showed that this strain produces γ-carotene, as well as glycosylated and acyl-glycosylated derivatives of 1′-hydroxy-4-keto-gamma-carotene . Strain 115-95T is catalase-positive and hydrolyzes starch, gelatine, casein and tributyrin but not cellulose .
Data on the structure of the cell wall, quinones, cellular and polar lipids of strain 114-95T are not available, although H. giganteus Hp a2 was reported to produce menaquinones 6 and 7 . Members of the Chloroflexi do not contain a lipopolysaccharide-containing outer membrane and the peptidoglycan is a variant that usually contains L-ornithine as the diamino acid .
Genome sequencing and annotation
Genome project history
This organism was selected for sequencing as part of the DOE Joint Genome Institute Program DOEM 2005. The genome project is deposited in the Genomes On Line Database  and the complete genome sequence is deposited in GenBank. Sequencing, finishing and annotation were performed by the DOE Joint Genome Institute (JGI). A summary of the project information is shown in Table 2.
The history of strain 114-95T originates with J. G. Holt, who deposited the strain in the ATCC collection in 1961, from which it was distributed to the DSMZ and the CCUG .
Growth conditions and DNA isolation
H. aurantiacus strain 114-95T (DSM 785) was obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ). Cells for DNA isolation were grown at 28 °C in the recommended CY liquid medium under oxic conditions with gentle shaking. DNA was isolated by the cetyl trimethylammonium bromide protocol recommended and described by the Joint Genome Institute . The purity, quality and size of the bulk gDNA preparation were assessed according to DOE-JGI guidelines  and were consistent with JGI quality-control standards.
Genome sequencing and assembly
The genome was sequenced using a combination of 3 kb, 8 kb and fosmid DNA libraries. All general aspects of library construction and sequencing can be found at the JGI website . The draft assembly contained 160 contigs in 51 scaffolds. The Phred/Phrap-/Consed software package was used for sequence assembly and quality assessment . Possible mis-assemblies were corrected with Dupfinisher . Gaps between contigs were closed by editing in Consed, custom priming, or PCR amplification. A total of 3,856 additional reactions and two shatter libraries were needed to close gaps and to raise the quality of the finished sequence. The error rate of the completed genome sequence is less than 1 in 100,000. Together, all libraries provided 11.0 × coverage of the genome. There are 85,815 total reactions in the final assembly.
Genes were identified using Prodigal  as part of the Oak Ridge National Laboratory genome annotation pipeline, followed by a round of manual curation using the JGI GenePRIMP pipeline . The predicted CDSs were translated and used to search the National Center for Biotechnology Information (NCBI) nonredundant database, UniProt, TIGRFam, Pfam, PRIAM, KEGG, COG, and InterPro databases. Additional gene prediction analysis and functional annotation was performed within the Integrated Microbial Genomes Expert Review (IMG-ER) platform 
The genome consists of a circular chromosome (6,346,587 bp) and two circular plasmids, pHAU01 (339,639 bp) and pHAU02 (88,204 bp), respectively with an overall G+C content of 50.9% (Table 3, Figure 3, Figure 4, Figure 5 and Figure 6). These are the only plasmids that have been identified to date among the seventeen Chloroflexi strains whose genomes have been sequenced. Interestingly, the GC content of the chromosome (50.7%) is notably lower than that of pHAU01 (53.7%) or pHAU02 (53.1%). Plasmid pHAU02 is predicted to encode 71 proteins, and pHAU01 is predicted to encode 231 potential proteins, which included a variety of transposases, phage recombinases and integrases, a CRISPR and CRISPR-associated gene cluster, and a variety of predicted transcription regulators. Neither plasmid encodes a product known to be essential for cell viability. Of the 5,654 genes predicted, 5,577 were protein-coding genes, and 77 encoded RNAs; 82 pseudogenes were identified. The majority of the protein-coding genes (67.4%) were assigned a putative function while the remaining ones were annotated as hypothetical proteins. The distribution of genes into COGs functional categories is presented in Table 4.
Insights into the genome
The phylum Chloroflexi is fascinatingly diverse and includes chlorophototrophs (Chloroflexales) as well as Gram-positive spore-forming organisms (Ktedonobacteria), reductive dehalogenating bacteria (Dehalococcoidetes), obligately aerobic heterotrophic thermophiles (Thermomicrobia), obligately anaerobic heterotrophs (Anaerolineae, Caldilineae), and marine organisms for which no example has yet been cultivated (e.g., SAR202 cluster). H. aurantiacus is described as an aerobic heterotroph from the order Herpetosiphonales, one of two orders within the class Chloroflexi. All characterized members of the other order, Chloroflexales, are chlorophototrophs. These include organisms from the genera Chloroflexus, Roseiflexus, Oscillochloris, Chloronema, Chlorothrix, and Heliothrix . An organism that appears to be most similar although not very closely related to members of Anaerolinea has also recently been shown to be chlorophototrophic . The genome sequence strongly supports the conclusion that H. aurantiacus is not a chlorophyll-based phototroph or an autotroph. Other than genes for carotenoid biosynthesis, a trait that is very widely distributed among members of all three kingdoms of life, no genes specifically used for photosynthetic light harvesting, electron transport, or chlorophyll biosynthesis were identified in the H. aurantiacus genome. Moreover, although Chloroflexus spp. and Roseiflexus spp. possess the genetic capacity to fix bicarbonate/CO2 by the 3-hydroxypropionate cycle, the corresponding genes are absent in H. aurantiacus [40,42].
Based upon the genes for carotenogenesis that are found in the H. aurantiacus genome, one can predict potential carotenoids that might be produced by this organism. The genome encodes homologs of crtB (phytoene synthase, crtI (phytoene desaturase), crtO (carotene 4-ketolase), crtD (hydroxyneurosporene dehydrogenase) and cruA (lycopene cyclase), and this combination of enzymes should allow this organism to produce γ-carotene derivatives with a keto-group at the 4 position. Additionally, the genome encodes cruF (γ-carotene 1′,2′-hydratase), cruC (1′-OH glycosyltransferase), and cruD, (acyl transferase) [43–45]. The presence of these genes would allow H. aurantiacus to synthesize monocyclic carotenoids carrying glycosyl, or glycosyl-fatty acyl ester moieties, attached to the 1′-OH group at the ψ–end of the molecule. This biosynthetic potential is in good agreement with the carotenoid contents of H. giganteus strain Hpa2  and Roseiflexus spp. strains. The former produces γ-carotene and its glycosyl and fatty acyl-glycoside ester derivatives, while the latter produce γ-carotene, 4-keto-myxocoxanthin-glucoside, and 4-keto-myxocoxanthin-glucoside fatty acyl esters [44,46].
Scanning of the H. aurantiacus genome for siderophore biosynthetic genes revealed the presence of a cluster with remarkable similarity to that of the myxobacterial iron chelator myxochelin . The annotated locus (Haur_01919–Haur_01928) contains a complete set of genes for the production of 2,3-dihydroxybenzoic acid, including open reading frames encoding isochorismate synthase (Haur_01921), isochorismate hydrolase (Haur_01923) and 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (Haur_01919). Additional genes in the operon are proposed to be involved in siderophore transport and iron utilization. Direct comparison with the myxochelin (mxc) cluster from Myxococcus xanthus DK1622 reveals the absence of an aminotransferase gene analogous to mxcL. Since mxcL is crucial for the formation of myxochelin B , H. aurantiacus probably only produces the biosynthetic predecessor mxyochelin A for iron sequestration. Homologs of genes involved in gliding motility in Myxococcus xanthus [48,49] and Flavobacterium johnsoniae [50–53] were not found in the H. aurantiacus genome. The genes required for gliding in H. aurantiacus and other members of the Chloroflexi have not yet been identified.
One of the most interesting and unique findings within the H. aurantiacus genome was the abundance of gene loci involved in secondary metabolism. Chloroflexi genomes were screened via BLASTP alignment against a representative library of conserved biosynthetic enzymes, a method proven useful in the analysis of the Salinispora tropica genome . Apart from genes involved in fatty acid and carotenoid biosynthesis, no hits were obtained for the chlorophototrophic Chloroflexi (Table 5). On the other hand, families of genes encoding enzymes of secondary metabolism were found to be highly overrepresented in the H. aurantiacus genome. A total of fourteen biosynthetic gene clusters were identified and annotated, 11 of which are specific for this organism (Table 6). The combined length of these clusters is estimated to be ∼448.6 kb, i.e. 6.6% of the H. aurantiacus genome is dedicated to natural product assembly. The biosynthetic capacity for secondary metabolite production of H. aurantiacus is thus comparable to that of other specialist producers of secondary metabolites, such as Actinobacteria and Myxobacteria [55,56]. The distribution of the biosynthetic loci on the H. aurantiacus chromosome seems non-random, with seven of them being located between 1.8 and 2.7 Mb clockwise from the replication origin, i.e. 50% of all clusters are concentrated in a region covering only 14% of the chromosome.
A striking feature of secondary metabolism in H. aurantiacus, which is also observed in myxobacterial and cyanobacterial genomes, is the preponderance of mixed polyketide synthase (PKS)-non-ribosomal peptide synthetase (NRPS) and NRPS gene clusters [56,57]. On the other hand, only two solely PKS systems were identified, including a type-III PKS of unknown function, as well as an iterative type-I PKS associated with enediyne biosynthesis (Table 6 and Figure 6). Enediyne clusters have as yet only been reported from Actinobacteria , and the presence of such a locus on the chromosome of H. aurantiacus is suggestive of horizontal gene transfer. Evidence for the recent acquisition of biosynthetic genes was obtained in case of the cluster designated pks-nrps5. This megacluster, which spans 112.6 kb of contiguous DNA on the chromosome (Table 6), is flanked both upstream and downstream by a number of transposon fragments. An above-average GC content of ∼66% as well as significant shifts of the latter in both border regions of the cluster suggest a foreign origin. The observation that horizontal gene transfer may account for the accumulation of biosynthetic genes is, to a degree, reminiscent of the evolution of the M. xanthus DK1622 genome .
Even though thiotemplate-based chemistry involving PKSs and NRPSs appears to be the predominant theme in natural product biosynthesis by H. aurantiacus, genomic analyses also revealed the molecular basis for the assembly and posttranslational modification of ribosomally encoded peptides [60,61]. Furthermore, various pathways to specific biosynthetic building blocks, such as the rare amino acid L-p-hydroxyphenylglycine  and the polyketide extender unit hydroxymalonyl-acyl carrier protein, were identified. The pathways are located adjacent to PKS and NRPS genes, which suggest that functional crosstalk is very likely to occur.
To date, the secondary metabolome of Herpetosiphon spp. has not been explored beyond a single report on a structurally complex, natural product, siphonazole, which likely derives from a mixed PKS-NRPS assembly line . The genome sequence of H. aurantiacus strain 114-95T now certainly provides a rationale for further studies of the chemistry and biology of this versatile microorganism.
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The work conducted by the U.S. Department of Energy Joint Genome Institute was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231 and was also supported by NSF grant MCB-0523100 to D.A.B. M.N. gratefully acknowledges the German Federal Ministry of Education and Research (BMBF 0315591A) for supporting research involving genomics within the GenoMik-Transfer framework.