- Extended genome report
- Open Access
Draft genome sequence of type strain HBR26T and description of Rhizobium aethiopicum sp. nov.
Standards in Genomic Sciencesvolume 12, Article number: 14 (2017)
Rhizobium aethiopicum sp. nov. is a newly proposed species within the genus Rhizobium. This species includes six rhizobial strains; which were isolated from root nodules of the legume plant Phaseolus vulgaris growing in soils of Ethiopia. The species fixes nitrogen effectively in symbiosis with the host plant P. vulgaris, and is composed of aerobic, Gram-negative staining, rod-shaped bacteria. The genome of type strain HBR26T of R. aethiopicum sp. nov. was one of the rhizobial genomes sequenced as a part of the DOE JGI 2014 Genomic Encyclopedia project designed for soil and plant-associated and newly described type strains. The genome sequence is arranged in 62 scaffolds and consists of 6,557,588 bp length, with a 61% G + C content and 6221 protein-coding and 86 RNAs genes. The genome of HBR26T contains repABC genes (plasmid replication genes) homologous to the genes found in five different Rhizobium etli CFN42T plasmids, suggesting that HBR26T may have five additional replicons other than the chromosome. In the genome of HBR26T, the nodulation genes nodB, nodC, nodS, nodI, nodJ and nodD are located in the same module, and organized in a similar way as nod genes found in the genome of other known common bean-nodulating rhizobial species. nodA gene is found in a different scaffold, but it is also very similar to nodA genes of other bean-nodulating rhizobial strains. Though HBR26T is distinct on the phylogenetic tree and based on ANI analysis (the highest value 90.2% ANI with CFN42T) from other bean-nodulating species, these nod genes and most nitrogen-fixing genes found in the genome of HBR26T share high identity with the corresponding genes of known bean-nodulating rhizobial species (96–100% identity). This suggests that symbiotic genes might be shared between bean-nodulating rhizobia through horizontal gene transfer. R. aethiopicum sp. nov. was grouped into the genus Rhizobium but was distinct from all recognized species of that genus by phylogenetic analyses of combined sequences of the housekeeping genes recA and glnII. The closest reference type strains for HBR26T were R. etli CFN42T (94% similarity of the combined recA and glnII sequences) and Rhizobium bangladeshense BLR175T (93%). Genomic ANI calculation based on protein-coding genes also revealed that the closest reference strains were R. bangladeshense BLR175T and R. etli CFN42T with ANI values 91.8 and 90.2%, respectively. Nevertheless, the ANI values between HBR26T and BLR175T or CFN42T are far lower than the cutoff value of ANI (> = 96%) between strains in the same species, confirming that HBR26T belongs to a novel species. Thus, on the basis of phylogenetic, comparative genomic analyses and ANI results, we formally propose the creation of R. aethiopicum sp. nov. with strain HBR26T (=HAMBI 3550T=LMG 29711T) as the type strain. The genome assembly and annotation data is deposited in the DOE JGI portal and also available at European Nucleotide Archive under accession numbers FMAJ01000001-FMAJ01000062.
Some bacteria are capable of forming a nitrogen-fixing symbiosis with various herbal and woody legumes. Some other bacterial species involve in nitrogen-fixation as free-living soil organisms . Biological nitrogen fixation by root-nodule forming bacteria in symbiosis with legume plants play significant roles in agricultural systems. The symbiosis provides a nitrogen source for the legumes and consequently improve legume growth and agricultural productivity.
Common bean ( Phaseolus vulgaris ) (http://plants.usda.gov/core/profile?symbol=PHVU) is one of the best-known legume plants cultivated worldwide for food. It was originally domesticated in its Mesoamerican gene center, including Mexico, Colombia, Ecuador and northern Peru  and in the Andean center in the regions from Southern Peru to northern Argentina . At present, it is widely cultivated in several parts of the tropical, sub-tropical and temperate agricultural systems  and used as a vital protein source mainly for low-income Latin Americans and Africans . In Ethiopia, beans are commonly grown as a sole crop or intercropped with cereals, such as sorghum and maize, at altitudes between 1400 and 2000 m above sea level . Bean plants make symbiotic associations promiscuously with several root-nodule forming nitrogen-fixing bacterial species commonly known as rhizobia. Studies thus far show that this legume forms symbiotic associations mainly with rhizobia belong to Alphaproteobacteria , such as Rhizobium phaseoli , Rhizobium tropici , Rhizobium leguminosarum , Rhizobium etli , Rhizobium giardinii , Rhizobium gallicum , Rhizobium leucaenae , Rhizobium lusitanum [11 ], Rhizobium vallis , Rhizobium ecuadorense . Rhizobium mesoamericanum , Rhizobium freirei , Rhizobium azibense , Rhizobium acidisoli , Ensifer meliloti , Ensifer fredii , Ensifer medicae  and Ensifer americanum . Rhizobial species belonging to Betaproteobacteria , such as Burkholderia phymatum  was also found capable of forming nodules on common bean plants.
16S rRNA gene sequence similarity and DNA–DNA hybridization techniques have been used as standard methods for describing new bacterial species. However, the 16S rRNA gene sequence divergence between closely related species is low and thus cannot differentiate closely related species found in the same genus [23,24,25]. The DDH technique was once considered as the gold standard method, and strains classified in the same species should have 70% or greater DDH relatedness among each other [26,27,28,29]. However, DDH results vary between different laboratories and this incurs inconsistent classification of the same species . On the other hand, the multilocus sequence analysis method, using the sequences of several housekeeping protein coding genes, have been successfully used for species identification and delineation [24, 25, 31, 32]. The genome-wide ANI method, which was first proposed by Konstantinidis and Tiedje  has recently successfully been used for classification of various bacterial species [34, 35]. Depending on the methods used for ANI calculation or the nature of bacterial genome sequences, 95 or 96.5% ANI value [34, 35] corresponds to the classical 70% DNA–DNA relatedness cutoff value for strains of the same species. The advancement of sequencing techniques and its falling price have made genomic data for many bacterial species available for comparison . Consequently, the ANI is becoming the method of choice in current bacterial taxonomic studies.
In our previous study, we isolated a group of rhizobial bacteria from nodules of common bean growing in the soils of Ethiopia. These bacteria formed a unique branch that was distinct from recognized species of the genus Rhizobium in phylogenetic trees constructed based on MLSA . In order to compare strains using genome-wide ANI with reference genomes and to describe this group as a new Rhizobium species, the representative strain Rhizobium sp. HBR26 (hereafter Rhizobium aethiopicum sp. nov. HBR26 T) was selected for sequencing. This project was a part of the DOE JGI 2014 Genomic Encyclopedia of Type Strains, Phase III, the genomes of soil and plant-associated and newly described type strains sequencing program . In this study, we present classification and general features of R. aethiopicum sp. nov. including the description of the genome sequence and annotation of the type strain HBR26 T.
Classification and features
The strain HBR26 T is the type strain of R. aethiopicum sp. nov. This strain and other strains in the novel species were isolated from nodules of common bean plants in Ethiopia. Based on multiple housekeeping gene analysis, the closest validly published species was R. etli . In this study, a partial 16S rRNA gene tree was constructed by retrieving more and recently published reference sequences from the GenBank database. In the phylogenetic tree, the novel species grouped together and showed high 16S rRNA gene sequence similarity (99%) with strains in the neighbor groups R. etli CFN42 T, Rhizobium vallis CCBAU65647 T, Rhizobium phaseoli CIAT652, Rhizobium pisi DSM30132 T, Rhizobium binae BlR195T, and R. bangladeshense BLR175T (Fig. 1). We also analyzed the housekeeping genes recA and glnII to resolve the relationships between strains in novel species and known species in the R. leguminosarum complex group . In the phylogenetic tree reconstructed based on the concatenated sequences, the novel species formed a clearly distinct group branching from the rhizobial species R. etli and R. bangladeshense (Fig. 2). This result was in agreement with our previous tree produced from concatenated partial 16S rRNA, recA, rpoB and glnII gene sequences . Strain HBR26 T and other strains in the novel species showed high recA and glnII gene sequence (892 bp) similarities among each other. The similarities between HBR26 T and the type strains R. etli CFN42 T and R. bangladeshense BLR175T ranged from 93 to 94%, CFN42 T being the closest type strain with a sequence similarity of 94%.
Minimum Information about the Genome Sequence is provided in Table 1 and the Additional file 1: Table S1. R. aethiopicum sp. nov. HBR26 T is fast-growing, forming moist, raised and smooth colonies 3–5 mm in diameter within 3–4 days on YEM agar plates at 28 °C. It is able to grow in the 15 °C to 30 °C temperature range, but its optimal growth was at 28 °C. The organism is able to grow at NaCl concentrations of 0–0.5% and at pH values in the range 5–10. Growth at pH4, at 4 °C and at 37 °C, and in 1-5% NaCl was recorded negative (Additional file 1: Table S1). This bacterial species is Gram-negative and rod shaped with a size of 1.0-2.4 μM in length (Fig. 3). HBR26 T and other strains in the novel species were able to respire many carbon sources when assessed by Biolog GN2 plates following the manufacturer’s instructions . In brief, colonies grown on YEM agar were transferred to and incubated for 48–96 h at 28 °C on freshly prepared R2A media consisting of yeast extract 0.5 g, proteose peptone 0.5 g, casamino acids 0.5 g, glucose 0.5 g, soluble starch 0.5 g, sodium pyruvate 0.3 g, K2HPO4 0.3 g, MgSO4.7H2O 0.05 g, and noble agar 15 g per liter of distilled H2O at pH7.2. Then colonies were suspended in 0.5% (w/v) saline (turbidity level of 52% transmittance), and 150 μl of the saline suspension was transferred to each of 96 wells of the Biolog GN2 Microplate. The plates were incubated at 28 °C, and results were checked after 4, 24, and 48 h. Positive results were recorded when the wells turned purple. All tested R. aethiopicum sp. nov. strains could respire 40 of the substrates in common, but 21 carbon sources were not respired by any of the tested strains. While the test strains did not show much diversity among themselves in substrate utilization pattern, they were distinctly different from carbon source respiration pattern of the closest reference R. etli CFN42 T; the test strains responded positively for seven carbon sources that were not used by R. etli CFN42 T. Substrates D-galactonic acid, lactone, sebacic acid and D- and L-α-glycerol phosphate were used exclusively by HBR26 T. Quinic acid and glycyl-L-aspartic acid were used solely by R. aethiopicum sp. nov. HBR31. The details of carbon source assimilation results are presented in Additional file 2: Table S2.
HBR26 T including other strains in the R. aethiopicum sp. nov. are nodule forming and nitrogen-fixing on common bean host plants. The strains were originally isolated from root nodules of common bean plants growing in soils of Ethiopia . In this study, the nodulation and nitrogen fixation capability was tested on legumes plants common bean, faba bean ( Vicia faba ) (http://plants.usda.gov/core/profile?symbol=VIFA), field pea ( Pisum sativum ) (http://plants.usda.gov/core/profile?symbol=PISA6) and lentil ( Lens culinaris ) (http://plants.usda.gov/core/profile?symbol=LECU2) on a sand, vermiculite and gravel mixture plant medium (5:3:3 ratio, respectively) in a growth chamber as previously described . The test revealed that the strains were able to form effective nitrogen-fixing nodules in symbioses with common bean host plants. Nevertheless, the strains were not able to form symbiotic associations with faba bean, field pea and lentil. The nodulation and symbiotic characteristics results are summarized in Additional file 1: Table S1.
Genome sequencing information
Genome project history
In our previous study , the organism showed a unique phylogenetic position which most likely represented a new species. Thus, it was chosen for genome sequencing in order to describe a new species by comparing its genome sequence with the genome sequences of other close Rhizobium species. This project was a part of the DOE JGI 2014 Genomic Encyclopedia of Type Strains, Phase III the genomes of soil and plant-associated and newly described type strains sequencing program. The genome project is deposited at the DOE JGI genome portal  and also available at European Nucleotide Archive  under accession numbers FMAJ01000001-FMAJ01000062. Sequencing, assembling, and annotation were done by the DOE JGI. A summary of the genome project information is listed in Table 2.
Growth conditions and genomic DNA preparation
First HBR26 T (=HAMBI 3550 T=LMG 29711 T) was grown aerobically on YEM agar plates at 28 °C. A pure colony was transferred into 3 ml YEM broth medium and the cell culture was grown for four days in a shaker incubator (200 rpm) at 28 °C. One ml was used to inoculate 150 ml YEM broth, and cells were grown on a shaker (200 rpm) again at 28 °C until the culture reached late-logarithmic phase. DNA was isolated from cell pellets collected in a 60 ml following the CTAB bacterial genomic DNA isolation protocol Version Number 3 provided by the DOE JGI .
Genome sequencing and assembly
The genome was sequenced at the DOE JGI using a combination of Illumina HiSeq 2500 and Illumina HiSeq 2500-1 TB technologies . An Illumina standard shotgun library was constructed and sequenced using the Illumina HiSeq 2000 platform which generated 9,310,748 reads totaling 1405.9 Mbp. Methods used for library construction and sequencing can be found at the DOE JGI website . In order to discard artifacts from Illumina sequencing and library preparation, all raw Illumina sequence data was passed through the program DUK at DOE JGI . Filtered Illumina reads were assembled using Velvet (version 1.2.07)  and then from Velvet contigs, 1–3 kb simulated paired-end reads were constructed using wgsim (version 0.3.0) (https://github.com/lh3/wgsim). Allpaths–LG (version r46652)  was used to assemble Illumina reads with a simulated read. The final assembly was based on 1,290.5 Mbp of Illumina data, which provides 258.1× input read coverage of the genome. The draft genome is 6.6 Mbp in size and contains 64 contigs in 62 scaffolds.
Genes were predicted using Prodigal  and using the DOE JGJ annotation pipeline . The identified protein-coding genes were translated and functionally annotated by comparing the sequences with the NCBI non-redundant database, UniProt, TIGRFam, Pfam, KEGG, COG, and InterPro databases. The tRNA genes were found using tRNAScanSE tool  and ribosomal RNA genes were identified by searches against models of the ribosomal RNA genes at the SILVA database . Other non–coding RNAs such as the RNA components of the protein secretion complex and the RNase P were identified by searching the genome for the corresponding Rfam profiles using INFERNAL . Additional analysis was accomplished using the IMG tool . The same tool was also used for manual functional annotation of the predicted genes and for examining the genome sequence.
The genome of HBR26 T is arranged in 62 scaffolds and consists of 6,557,588 bp, with a 61% G + C content. In total 6307 genes were predicted, of these 6221 were protein-coding genes and 86 were RNA genes. Five rRNAs identified including one 16S rRNA, two 5S rRNA, and two 23S rRNA genes. There were 52 tRNA genes and 29 other (miscRNA) RNA genes. The statistics and properties of the genome are summarized in Table 3. The majority of the protein-coding genes, 5054 (80.13%) were assigned with putative functions (Table 3), and of these 4578 genes (72.59%) were assigned to COG functional categories (Table 4). The remaining genes were annotated as hypothetical proteins (1167 genes, 18.5%).
Insights from the genome sequence
Genome wide comparative analysis
Based on recA-glnII concatenated sequence comparisons, the proposed type strain HBR26 T and strains included in R. aethiopicum sp. nov., HBR23, HBR3, HBR31, HBR7, and HBR50 were closely related to each other (99–100% sequence identity). Nevertheless, these strains were only distantly related to the closest reference strains R. etli CFN42 T (94%) and R. bangladeshense BLR175T (93%). In order to further resolve the taxonomy of the novel group, genomic comparative analyses were done between HBR26 T and several relatively close reference strains presented in the Fig. 2. For this the genomes of a number strains, such as R. etli CFN42 T, Rhizobium etli IE4771, Rhizobium etli Mim1, Rhizobium etli IE4803, Rhizobium phaseoli Ch24-10, Rhizobium phaseoli CIAT652, Rhizobium acidisoli FH23, Rhizobium ecuadorense PSO671 T, and Rhizobium leguminosarum CB782, CCGM1, WSM2304, PM1131, WSM1325, 4292, 3841, and UPM1137 were retrieved from the DOE JGI genome portal (Tables 5 and 6). ANI was computed from protein-coding genes of the genomes using the MiSI program implemented in the IMG database . For a pair of genome sequences, the system calculates ANI by averaging the nucleotide identity of orthologous genes identified as bidirectional best hits and also calculates Alignment Fraction of orthologous genes . In addition, partially sequenced genome reads from R. bangladeshense BLR175T, Rhizobium lentis BLR27T, Rhizobium binae BLR 95T, Rhizobium anhuiense CCBAU23252 T, R. pisi DSM30132 T and Rhizobium fabae CCBAU33202 T were used for calculation of additional ANI with the JSpecies program using default parameters as previously used [52, 53]. Table 5 shows the ANI values obtained between HBR26 T and reference strains (numbers above the diagonal). The numbers below the diagonal show pairwise orthologous genes identified as bidirectional best hits between genomes. AF was >0.68 in all ANI calculations among whole or draft genomes but the AF value was <0.6 in all ANI calculations with partially sequenced genome reads. The ANI values obtained between HBR26 T and references strains varied between 87.4 and 91.8%, which was below 96%, the value of relatedness recommended for species delineation . The closest strains were R. bangladeshense LR175T and R. etli CFN42 T with ANI values 91.8 and 90.2%, respectively. This result is in agreement with the recA-glnII concatenated analysis (Fig. 2), confirming that that HBR26 T is distantly related to the R. etli and R. bangladeshense species but belongs to the novel Rhizobium species. The ANI between R. etli IE4803 and R. etli IE4771 was 97.7%. However, ANI values between these strains and the type strain R. etli CFN42 T (= < 90.2%) was much below the cutoff value of strains of the same species. Several R. leguminosarum strains included in Table 5 may represent species other than R. leguminosarum (ANI < 96% each other). The genome of R. leguminosarum CCGM1 showed a significantly higher degree of similarity with R. phaseoli Ch24-10 (97.2% ANI) and CIAT652 (97.2% ANI), and could thus be classified as R. phaseoli . R. leguminosarum WSM2304 showed 96.6% genomic relatedness with R. acidisoli FH23T. Accordingly, we suggest the classification of WSM2304 under R. acidisoli species. The ANI value between R. fabae CCBAU33202 T and R. pisi DSMZ30132 T was 96.6%. This value corroborates the relationship between the two strains as reported previously , which is also shown in the recA-glnII based phylogenetic tree in Fig. 2, suggesting that R. fabae CCBAU33202 T and R. pisi DSMZ30132 T might represent one and the same species.
Table 6 shows the genome statistics and functional category comparison between HBR26 T and close reference rhizobial strains. The draft genome of HBR26 T (6.6 Mbp) is about the same size as that of R. phaseoli Ch24-10 (6.6 Mbp) and slightly greater than R. etli CFN42 T (6.5 Mbp) and R. phaseoli CIAT652 (6.4 Mbp). However, strain HBR26 T has smaller genome size compared to R. leguminosarum CCGM1 (6.8 Mbp), R. etli IE4803 (6.9 Mbp), R. acidisoli FH23 (7.3Mbp), R. ecuadorense CNPSO671 (6.9Mbp) and all other R. leguminosarum (6.8-7.9 Mbp) symbiovar viciae and trifolii reference strains (Table 6). Though the gene content of strain HBR26 T (6307) is only greater than of CIAT652 (6132), it has got the highest percentage of genes assigned to Pfam (84.3%), TIGRfam (24.8%), and KEGG (29.7%). HBR26 T also has the highest percentage of genes assigned to COG (72.6%) and KOG (18.1%) functional categories, with the exceptions R. leguminosarum UPM1131 (72.9%), and WSM2304 (18.6%), respectively.
In Fig. 4 the Venn diagram plotted in the OrthoVenn program shows overlapping orthologous protein clusters between the genomes of HBR26 T and other common bean-nodulating references R. etli CFN42 T, and R. phaseoli Ch24-10, CIAT652 and CCGM1. The orthologous clusters were identified with default parameters, 1e-5 e-value cutoff for all protein similarity comparisons and 1.5 inflation value for the generation of orthologous clusters . In total the strains formed 6534 protein clusters, 6462 orthologous clusters (at least containing two strains) and 4273 single-copy gene clusters. All five strains shared in common 4385 orthologous protein clusters. On a pairwise basis, HB26T shares 32, 42 and 44 proteins with CCGM1, Ch24-10, and CIAT652, respectively. Strain HBR26 T shares the most with CFN42 T with 164 orthologous group. This result is in agreement with recA-glnII phylogenetic and ANI analysis, supporting that HBR26 T is more closely related to CFN42 T compared to the other bean-nodulating strains. The genome of HBR26 T contains the highest number of genome-specific proteins of the five strains with 665 singletons followed by CFN 42 T, CIAT652, Ch24-10 and CCGM1 with 568, 549 and 516 singletons, respectively.
Comparative analysis of accessary genes: emphasis on symbiotic genes
Genes which are not essentially present in all bacterial strains are known as accessory genes. These genes are contained by mobile elements such as plasmids, genomic islands, transposons or phages and thus can be gained or lost among bacterial strains through horizontal gene transfer mechanisms. Accessory genes in the genome of HBR26 T were searched by assembling against the reference genome R. etli CFN42 T using the Genome Gene Best Homologs package from program IMG-ER . Additional file 3: Table S3 shows homologous repABC (plasmid replication genes) and symbiotic genes found in the genome of HBR26 T. The result revealed that HBR26 T carries five different repABC genes homologous to the genes found in five of the R. etli CFN42 T  plasmids 42b, 42c, 42d, 42e, 42f, suggesting that HBR26 T may have five additional replicons other than the chromosome. The repABC genes corresponding to the symbiotic plasmid 42d showed high sequence similarity between other common bean nodulating strains CFN42 T, CIAT652, IE4803, Ch24-10, 4292 and CCGM1 (identity ranging 99–100%). This implies that bean- nodulating strains and HBR26 T may share common symbiotic plasmids. The HBR26 T repABC genes homologous to 42b, 42c, 42e and 42f also showed sequence similarity in the ranges 86–89%, 84–93%, 92–94%, 84–93%, respectively, with strains CIAT652, CFN42 T, IE4803, Ch24-10, 4292, CCGM1 and R. etli sv. mimosae Mim1.
The symbiosis between rhizobia and legume plants is initiated when plant exudates known as flavonoids trigger expression of the rhizobial nodulation genes that code for the synthesis of LCO Nod factors. The backbone of this LCO is encoded by the common nodABC accessory genes. There are also additional genes (nol, noe) which code for the substituent groups that decorate the LCO core . The symbiosis between rhizobia and legumes results in the formation of specialized organs on plant roots known as nodules in which rhizobia differentiate into N2-fixing bacteroids . Like most symbiotic rhizobia, the genome of HBR26 T carries the symbiotic genes encoding for the synthesis of LCO structures, substituent groups and genes coding for nitrogen fixation (Additional file 3: Table S3). Several of the nodulation and nitrogen-fixing genes are located on the scaffolds Ga0061105_135 and Ga0061105_130, 141, 144 and 150. The first scaffold contains the main nodulation genes except nodA, while the other scaffolds encompass many of the nitrogen-fixing genes (Additional file 3: Table S3).
The genomes of HBR26 T, R. etli CFN42 T, R. phaseoli Ch24-10 and CIAT652 were aligned using the progressive Mauve alignment tool , using default parameters. The genomic features were visualized using the Artemis Comparison Tool [60, 61]. The Mauve alignment in Fig. 5 shows the presence of a similar nodBCSIJD module organization between the genome of HBR26 T and the genomes of other bean-nodulating rhizobial strains CFN42 T, CIAT652, and Ch24-10. The nodDIJSCB genes are flanked by transposase genes and hypothetical protein-coding genes. A similar arrangement of the nod genes was also found in the genomes of CCGM1 and IE4803, which are also micro-symbionts of common bean (data not shown).
All HBR26 T, CFN42 T, Ch24-10, CIAT652, and CCGM1 genomes carry additional nodZ, noeI and nolE genes adjacent to the nodBCSIJD region. Similarly, in the genomes of clover and faba bean nodulating R. leguminosarum WSM2304, UPM1131 and 3841 the nodulation genes nodD, nodB, nodC, nodI, and nodJ are also clustered in the same region. In the latter case, this region contains additional nodA, nodL, nodE, and nodF genes as well. The nodA and nolL genes of HBR26 T which are located in the scaffolds Ga0061105_134 and Ga0061105_130, respectively, are very similar to the corresponding gene sequences of bean-nodulating rhizobial strains CFN42 T, Ch24-10, CCGM1, CIAT652 and IE4803 (99–100% similarity). Its nodB gene is also homologous with CFN42 T, CIAT652, and IE4803. The highest identity (100%) is with nodB of IE4803 followed by CFN42 T (98%) and CIAT652 (97%). nodC of HBR26 T shares 97% similarity with nodC of CIAT652, CFN42 T and, CCGM1. All nodS, nodI and nodJ genes of HBR26 T share high identity with those of CIAT652 (99%), CFN42 T (98%), CCGM1 (98%) and Ch24-10 (98%).
The nitrogenase complex, an enzyme responsible for nitrogen fixation in diazotrophs, consists of two components known as dinitrogenase and dinitrogenase reductase . The nif genes are required for the synthesis and functioning of the nitrogenase complex . Many of these genes in the genome of HBR26 T are harbored in four different scaffolds Ga0061105_130, Ga0061105_150, Ga0061105_144, and Ga0061105_141. The first scaffold contains the nifA-nifB-nifT-nifZ-nifW genes, and the second scaffold includes the nifE, nifN and nifX genes. The nitrogen-fixing genes nifH, nifU and nifQ are retained in the scaffold Ga0061105_141. An additional nifH gene, fixG and fixH genes are found in the scaffold Ga0061105_144 and a nifK gene is located in the scaffold Ga0061105_162. The dinitrogenase component of the nitrogenase complex is a product of nifD and nifK genes and the dinitrogenase reductase is coded by nifH . However, the nifD gene is missing in the draft genome of HBR26 T. This gene is important to enable the nitrogenase enzyme complex functional. On the other hand, the strain HBR26 T makes effective nitrogen-fixing symbiosis with common bean plants. Thus, the reason behind the absence of nifD in the genome of HBR26 T is probably because our data is a draft genome and probably nifD was missed during sequencing. It is also possible that nifD sequence was truncated when the library was constructed.
The genes nifB, nifT, nifZ, nifE, nifN, nifX, fixG, fixH, nifW, nifQ, nifK and nifH all share high identity with homologous genes found in CFN42 T (98–100%), Ch24-10 (98–100%), CCGM1 (98–100%), 4292 (96–99%) or in IE4803 (92–100%). In our previous study, we identified rhizobial strains belong to R. phaseoli , R. etli and R. leguminosarum from root nodules of common bean plants growing in the soils of Ethiopia . Thus, the close similarity of the nod, nif and fix genes between HBR26 T and bean-nodulating R. etli , R. phaseoli and R. leguminosarum strains suggests that those genes might be shared between these rhizobial species through horizontal gene transfer mechanisms.
This study presents the genome sequence for the R. aethiopicum sp. nov. strain HBR26 T. The result from phylogenetic analyses of multilocus sequences of core genes showed a novel species within the genus Rhizobium . This result was further supported by ANI calculation, in which the genome of the type strain HBR26 T exhibited < 91.8% identity when compared with the genomes of close Rhizobium species. This value is much lower than the 96% ANI limit for delineating a species. The data confirms that R. aethiopicum sp. nov. should be considered as a new Rhizobium species. Thus, on the basis of phylogenetic, comparative genomic analyses and ANI results and by including phenotypic characteristics, we formally propose the creation of R. aethiopicum sp. nov. that contains the strain HBR26 T (= HAMBI 3550 T=LMG 29711 T). The strains included in this species are effective nitrogen-fixing rhizobia in symbiosis with common bean plants. The genome of the type strain HBR26 T carries five plasmid replication repABC genes homologous to the genes found in five of the R. etli CFN42 T plasmids, suggesting that HBR26 T may have five additional replicons other than the chromosome. The organization of nodBCSIJD genes is similar between the genomes of HBR26 T and other bean-nodulating rhizobial species. The symbiotic genes necessary for nodulation and for nitrogen fixation share high sequence similarity between bean-nodulating strains, such as R. etli , R. phaseoli and R. leguminosarum , which suggests that these genes might be shared between bean-nodulating rhizobial species through horizontal gene transfer mechanisms.
Description of Rhizobium aethiopicum sp. nov.
Rhizobium aethiopicum (ae.thi.o’pic.um. L. neut. adj. aethiopicum, pertaining to Ethiopia). Fast-growing, forming moist, raised and smooth colonies 3–5 mm in diameter within 3–4 days on YEM agar plates under optimal growth conditions, at 28 °C and pH7. The strains are able to grow between 15 °C and 30 °C. The organisms require no or trace amounts of NaCl for growth and are only able to grow at NaCl concentrations of 0–0.5% and at pH values in the range 5–10. No growth occurred at pH4, at temperature 4 °C and at 37 °C, and 1–5% NaCl. Cells are Gram-negative rod-shaped and 1.0–2.4 μM in length. Oxidation of the following substrates as carbon sources in Biolog GN2 microplates was recorded positive; dextrin, glycogen, N-acetyl-D-glucosamine, adonitol, L-arabinose, D-arabitol, D-cellobiose, I-erythritol, D-fructose, L-fucose, D-galactose, α-D-glucose, α-D-lactose, lactulose, maltose, D-mannitol, D-mannose, D-melibiose, β-methyl-D-glucoside, D-psicose, D-raffinose, L-rhamnose, D-sorbitol, sucrose, D-trehalose, turanose, xylitol, pyruvic acid methyl ester, succinic acid mono-methyl-ester, β-hydroxybutyric acid, γ-hydroxybutyric acid, itaconic acid, α-keto butyric acid, α-keto glutaric acid, D,L-lactic acid, succinic acid, bromo-succinic acid, succinamic acid, L-alaninamide, D-alanine, L-alanine, L-alanyl-glycine, L-asparagine, L-aspartic acid, L-glutamic acid, glycyl-L-glutamic acid, L-histidine, hydroxy-L-proline, L-ornithine, L-proline, D,L-carnitine, γ-amino butyric acid, urocanic acid, nosine, uridine, thymidine, glycerol, α-d-glucose-1-phosphate and D-glucose-6-phosphate. However, the oxidation was negative for the following substrates: α-cyclodextrin, Tween 40, Tween 80, N-acetyl-D-galactosamine, gentiobiose, acetic acid, D-galacturonic acid, D-gluconic acid, D-glucosaminic acid, D-glucuronic acid, p-hydroxy phenylacetic acid, α-keto valeric acid, propionic acid, D-saccharic acid, glucuronamide, L-phenylalanine, L-pyroglutamic acid, D-serine, phenyethyl-amine, putrescine, and 2-aminoethanol. The type strain HBR26 T (= HAMBI 3550 T =LMG 29711 T) was isolated from root nodules of common bean plants growing in Ethiopia. The genome size of the type strain is 6.6 Mbp and the G + C content of the genome is 61%. The genome sequence of the type strain is deposited at DOE JGI genome portal under IMG genome/Taxon ID: 2615840624  and also available at European Nucleotide Archive  under accession numbers FMAJ01000001-FMAJ01000062. The type strain has been deposited in the HAMBI (HAMBI 3550 T) and LMG (LMG 29711 T) culture collections.
Average nucleotide identity values
Cetyl Trimethyl Ammonium Bromide
Department of energy
Genomes online database
Integrated microbial genomes
Integrated microbial genomes – expert review
Joint Genome Institute
Minimum information about a genome sequence
Microbial species identifier
Multilocus sequence analysis
- N2 :
Reasoner’s 2A Agar
Yeast Extract Mannitol
Franche C, Lindström K, Elmerich C. Nitrogen-fixing bacteria associated with leguminous and non-leguminous plants. Plant Soil. 2009;321:35–59.
Gepts P, Debouck D. Origin, domestication and evolution of the common bean (Phaseolus vulgaris L.). In: van Schoonhoven A, Voysest O, editors. Common beans, research for crop improvement. Wallingford, UK, Cali-Colombia: CAB; 1991. p. 7–53.
Kaplan L. Archeology and domestication in American Phaseolus (Beans). Econ Bot. 1965;19:558–68.
Singh SP. Production and utilization. In: Singh SP, editor. Common bean improvement in the twenty-first century. Boston: Kluwer Academic Publishers; 1999. p. 1–24.
Ribeiro MC, Metzger JP, Martensen AC, Ponzoni FJ, Hirota MM. The Brazilian Atlantic Forest: how much is left, and how is the remaining forest distributed? Implications for conservation. Biol Conserv. 2009;142:1141–53.
Assefa T, Assefa H, Kimani P. Development of improved haricot bean germplasm for the mid and low-altitude sub-humid agroecologies of Ethiopia. In: Ali K, Ahmed S, Beniwal S, Kenneni G, Rajandra SM, Makkouk K, editors. Workshop on food and forage legumes of Ethiopia. Aleppo: ICARDA; 2006. p. 87–94.
Martínez-Romero E, Segovia L, Mercante FM, Franco AA, Graham P, Pardo MA. Rhizobium tropici, a novel species nodulating Phaseolus vulgaris L. beans and Leucaena sp. trees. Int J Syst Bacteriol. 1991;41:417–26.
Segovia L, Young JPW, Martinez-Romero E. Reclassification of American Rhizobium leguminosarum biovar phaseoli type I strain as Rhizobium etli sp. nov. Int J Syst Bacteriol. 1993;43:374–7.
Amarger N, Macheret V, Laguerre G. Rhizobium gallicum sp. nov. and Rhizobium giardinii sp. nov. from Phaseolus vulgaris nodules. Int J Syst Bacteriol. 1997;47:996–1006.
Ribeiro RA, Rogel MA, Lòpez-Lòpez A, Ormen˜o-Orrillo E, Barcellos FG, Martínez J, Thompson FL, Martínez-Romero E, Hungria M. Reclassification of Rhizobium tropici type A strains as Rhizobium leucaenae sp. nov. Int J Syst Evol Microbiol. 2012;62:1179–84.
Valverde A, Igual JM, Peix A, Cervantes E, Velázquez E. Rhizobium lusitanum sp. nov. a bacterium that nodulates Phaseolus vulgaris. Int J Syst Evol Microbiol. 2006;56:2631–7.
Wang F, Wang ET, Wu LJ, Sui XH, LiJr Y, Chen WX. Rhizobium vallis sp. nov., isolated from nodules of three leguminous species. Int J Syst Evol Microbiol. 2011;61:2582–8.
Ribeiro RA, Martins TB, Ormeño-Orrillo E, et al. Rhizobium ecuadorense sp. nov., an indigenous N2-fixing symbiont of the Ecuadorian common bean (Phaseolus vulgaris L.) genetic pool. Int J Syst Evol Microbiol. 2015;65:3162–9.
Lopez-Lopez A, Rogel-Hernandez MA, Barois I, et al. Rhizobium grahamii sp. nov., from nodules of Dalea leporina, Leucaena leucocephala and Clitoria ternatea, and Rhizobium mesoamericanum sp. nov., from nodules of Phaseolus vulgaris, siratro, cowpea and Mimosa pudica. Int J Syst Evol Microbiol. 2012;62:2264–71.
Dall’Agnol RF, Ribeiro RA, Ormeno-Orrillo E, et al. Rhizobium freirei sp. nov., a symbiont of Phaseolus vulgaris that is very effective at fixing nitrogen. Int J Syst Evol Microbiol. 2013;63:4167–73.
Mnasri B, Liu TY, Saidi S, et al. Rhizobium azibense sp. nov., a nitrogen fixing bacterium isolated from root nodules of Phaseolus vulgaris. Int J Syst Evol Microbiol. 2014;64:1501–6.
Román-Ponce B, Zhang JY, Vásquez-Murrieta MS, et al. Rhizobium acidisoli sp. nov., isolated from root nodules of Phaseolus vulgaris in acid soils. Int J Syst Evol Microbiol. 2016;66:398–406.
Mnasri B, Moncef M, Gisèle L, Mohamed A, Ridha M. Salt-tolerant rhizobia isolated from a Tunisian oasis that are highly effective for symbiotic N2-fixation with Phaseolus vulgaris constitute a novel biovar (bv. mediterranense) of Sinorhizobium meliloti. Arch Microbiol. 2007;187(1):79–85.
Sadowsky MJ, Cregan PB, Keyser HH. Nodulation and nitrogen fixation efficiency of Rhizobium fredii with Phaseolus vulgaris genotypes. Appl Environ Microbiol. 1988;54:1907–10.
Mhamdi R, Laguerre G, Aouani ME, et al. Different species and symbiotic genotypes of field rhizobia can nodulate Phaseolus vulgaris in Tunisian soils. FEMS Microbiol Ecol. 2012;41:77–84.
Toledo I, Lloret L, Martinez-Romero E. Sinorhizobium americanus sp. nov., a new Sinorhizobium species nodulating native Acacia spp. in Mexico. Syst Appl Microbiol. 2003;26:54–64.
Talbi C, Delgado MJ, Girard L, Ramírez-Trujillo A, Caballero-Mellado J, Bedmar EJ. Burkholderia phymatum strains capable of nodulating Phaseolus vulgaris are present in Moroccan soils. Appl Environ Microbiol. 2010;76:4587–91.
Amann RI, Lin CH, Key R, Montgomery L, Stahl DA. Diversity among fibrobacter strains: towards a phylogenetic classification. Syst Appl Microbiol. 1992;15:23–32.
Aserse AA, Räsänen LA, Assefa F, Hailemariam A, Lindström K. Phylogeny and genetic diversity of native rhizobia nodulating common bean (Phaseolus vulgaris L.) in Ethiopia. Syst Appl Microbiol. 2012;35(2):120–31.
Martens M, Dawyndt P, Coopman R, Gillis M, De Vos P, Willems A. Advantages of Multilocus sequence analysis for taxonomic studies: a case study using 10 housekeeping genes in the genus Ensifer. Int J Syst Evol Microbiol. 2008;58:200–14.
Germano MG, Menna P, Mostasso FL, Hungria M. RFLP analysis of the RNA operon of a Brazilian collection of bradyrhizobial strains from thirty three legume species. Int J Syst Evol Microbiol. 2006;56:217–29.
Stackebrandt E, Frederiksen W, Garrity GM, Grimont PAD, Kämpfer P, Maiden MCJ, Nesme X, Rosselló-Mora, Swings R, et al. Report of the ad hoc committee for the reevaluation of the species definition in bacteriology. Int J Syst Evol Microbiol. 2002;52:1043–7.
Vinuesa P, Leon-Barrios M, Silva C, Willems A, Jarabo-Lorenzo A, Perez-Galdona R, Werner D, Martinez-Romero E. Bradyrhizobium canariense sp. nov., an acid-tolerant endosymbiont that nodulates genistoid legumes (Papilionoideae: Genisteae) from the Canary Islands, along with Bradyrhizobium japonicum bv. genistearum, Bradyrhizobium genospecies a and Bradyrhizobium genospecies b. Int J Syst Evol Microbiol. 2005;55:569–75.
Willems A, Munive A, de Lajudie P, Gillis M. In most Bradyrhizobium groups sequence comparison of 16S–23S rDNA internal transcribed spacer regions corroborates DNA–DNA hybridizations. Syst Appl Microbiol. 2003;26:203–10.
Rosselló-Mora R. DNA-DNA re-association methods applied to microbial taxonomy and their critical evaluation. In: Stackebrandt E, editor. Molecular identification, systematics and population structure of prokaryotes. Berlin: Springer; 2006. p. 23–50.
Mousavi SA, Willems A, Nesme X, de Lajudie P, Lindström K. Revised phylogeny of Rhizobiaceae: Proposal of the delineation of Pararhizobium gen. nov., and 13 new species combinations. Syst Appl Microbiol. 2015;38(2):84–90.
Mousavi SA, Osterman J, Wahlberg N, Nesme X, Lavire C, Vial L, Paulin L, de Lajudiee P, Lindström K. Phylogeny of the Rhizobium-Allorhizobium-Agrobacterium clade supports the delineation of Neorhizobium gen. nov. Syst Appl Microbiol. 2014;37(3):208–15.
Konstantinidis KT, Tiedje JM. Genomic insights that advance the species definition for prokaryotes. Proc Natl Acad Sci U S A. 2005;102:2567–72.
Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol. 2014;64:346–51.
Varghese JN, Mukherjee S, Ivanova N, Konstantinidis KT, Mavrommatis K, Kyrpides NC, Pati A. Microbial species delineation using whole genome sequences. Nucleic Acids Res. 2015;43(14):6761–71.
Ramasamy D, Mishra AK, Lagier JC, Padhmanabhan R, Rossi M, Sentausa E, Raoult D, Fournier PE. A polyphasic strategy incorporating genomic data for the taxonomic description of novel bacterial species. Int J Syst Evol Microbiol. 2014;64:384–91.
Whitman WB, Woyke T, Klenk HP, Zhou Y, Lilburn TG, Beck BJ, De Vos P, Vandamme P, Eisen JA, Garrity G, Hugenholtz P, Kyrpides NC. Genomic Encyclopedia of Bacterial and Archaeal Type Strains, Phase III: the genomes of soil and plant-associated and newly described type strains. Stand Genomic Sci. 2015;10:26.
Biolog GN2 manufacturer’s instructions http://www.ecologiemicrobiennelyon.fr/IMG/pdf/GN2-1.pdf).
DOE Joint Genome Institute. http://www.jgi.doe.gov/.
www.ebi.ac.uk/ena/data/view/Taxon:1138170 (European Nucleotide Archive).
CTAB DNA extraction protocol [http://jgi.doe.gov/collaborate-with-jgi/pmooverview/protocols-sample-preparation-information/].
Bennett S. Solexa Ltd. Pharmacogenomics. 2004;5(4):433–8.
Mingkun L, Copeland A, Han J. DUK, 2011. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4623924/pdf/40793_2015_Article_77.pdf. Accessed 26 Oct 2015.
Zerbino D, Birney E. Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res. 2008;18:821–9.
Gnerre S, MacCallum I. High–quality draft assemblies of mammalian genomes from massively parallel sequence data. PNAS. 2011;108(4):1513–8.
Hyatt D, Chen GL, Lacascio PF, Land ML, Larimer FW, Hauser LJ. Prodigal: prokaryotic gene recognition and translation initiation site identification. BMC Bioinf. 2010;11:119.
Huntemann M, Ivanova NN, Mavromatis K, Tripp HJ, Paez-Espino D, Palaniappan K, Szeto E, Pillay M, Chen IM-A, Pati A, Nielsen T, Markowitz VM, Kyrpides NC. The standard operating procedure of the DOE-JGI microbial genome annotation pipeline (MGAP v.4). Stand Genomic Sci. 2015;10:86.
Lowe TM, Eddy SR. tRNAscan–SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res. 1997;25:955–64.
Pruesse E, Quast C, Knittel, Fuchs B, Ludwig W, Peplies J, Glckner FO. SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Res. 2007;35:2188–7196.
INFERNAL. Inference of RNA alignments. http://infernal.janelia.org.
Markowitz VM, Chen IM, Palaniappan K, Chu K, Szeto E, Pillay M, Ratner A, Huang J, Woyke T, Huntemann M, Anderson I, Billis K, Varghese N, Mavromatis K, Pati A, Ivanova NN, Kyrpides NC. IMG 4 version of the integrated microbial genomes comparative analysis system. Nucleic Acids Res. 2014;42(1):D560–7.
Kurtz S, Phillippy A, Delcher AL, Smoot M, Shumway M, Antonescu C, Salzberg SL. Versatile and open software for comparing large genomes. Genome Biol. 2004;5:R12.
Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A. 2009;106:19126–31.
Wang Y, Coleman-Derr D, Chen G, Gu YQ. OrthoVenn: a web server for genome wide comparison and annotation of orthologous clusters across multiple species. Nucleic Acids Res. 2015;43(W1):W78–84. doi:10.1093/nar/gkv487.
Markowitz VM, Mavromatis K, Ivanova NN, Chen IM, Chu K, Kyrpides NC. IMG-ER: a system for microbial genome annotation expert review and curation. Bioinformatics. 2009;25:2271–8.
González V, Santamaría RI, Bustos P, Hernández-González I, Medrano-Soto A, Moreno-Hagelsieb G, Janga SC, Ramírez MA, Jiménez-Jacinto V, Collado-Vides J, Dávila G. The partitioned Rhizobium etli genome: genetic and metabolic redundancy in seven interacting replicons. Proc Natl Acad Sci U S A. 2006;103:3834–9.
Perret X, Staehelin C, Broughton WJ. Molecular basis of symbiotic promiscuity. Microbiol Mol Biol Rev. 2000;64:180–201.
Terpolilli JJ, Hood GA, Poole PS. What determines the efficiency of N2-fixing Rhizobium-legume symbioses? Adv Microb Physiol. 2012;60:325–89.
Darling AE, et al. Progressive Mauve: multiple genome alignment with gene gain, loss and rearrangement. PLoS One. 2010;87(12):4576-9.
Camacho C, Coulouris G, Avagyan V, Ma N, Papadopoulos J, Bealer K, Madden TL. BLAST+: architecture and applications. BMC Bioinf. 2009;10:421. doi:10.1186/1471-2105-10-421.
Carver T, et al. BamView: viewing mapped read alignment data in the context of the reference sequence. Bioinformatics. 2010;26:676–7.
Rubio LM, Ludden PW. Biosynthesis of the iron-molybdenum cofactor of nitrogenase. Annu Rev Microbiol. 2008;62:93–111.
Field D, Garrity G, Gray T, Morrison N, Selengut J, Sterk P, et al. The minimum information about a genome sequence (MIGS) specification. Nat Biotechnol. 2008;26:541–7.
Woese CR, Kandler O, Wheelis ML. Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya. PNAS USA. 1990;87:4576–9.
Garrity GM, Bell JA, Lilburn T. Phylum XIV. Proteobacteria phyl. nov. In: Garrity GM, Brenner DJ, Krieg NR, Staley JT, editors. Bergey’s manual of systematic bacteriology, vol. Volume 2, Part B. Secondth ed. New York: Springer; 2005.
Garrity GM, Bell JA, Lilburn T. Class I. Alphaproteobacteria class. In: Garrity GM, Brenner DJ, Kreig NR, Staley JT, editors. Bergey’s manual of systematic bacteriology. 2nd ed. New York: Springer - Verlag; 2005.
Kuykendall LD. Order VI. Rhizobiales ord. nov. In: Garrity GM, Brenner DJ, Krieg NR, Staley JT, editors. Bergey’s manual of systematic bacteriology, vol. Volume 2, Part C. Secondth ed. New York: Springer; 2005. p. 324.
Skerman VBD, McGowan V, Sneath PHA. Approved lists of bacterial names. Int J Syst Bacteriol. 1980;30:225–420.
Young JM, Kuykendall LD, Martínez-Romero E, Kerr A, Sawada H. A revision of Rhizobium Frank 1889, with an emended description of the genus, and the inclusion of all species of Agrobacterium Conn 1942 and Allorhizobium undicola de Lajudie et al. 1998 as new combinations: Rhizobium radiobacter, R. rhizogenes, R. rubi, R. undicola and R. vitis. Int J Syst Evol Microbiol. 2001;51:89–103.
Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, et al. Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet. 2000;25:25–9.
Wang ET, Rogel MA, García-de los Santos A, Martínez-Romero J, Cevallos MA, Martínez-Romero E. Rhizobium etli bv. mimosae, a novel biovar isolated from Mimosa affinis. Int J Syst Bacteriol. 1999;49:1479–91.
All microbiological lab work, data analyses, and manuscript preparation were supported by the SOILMAN project funded by Academy of Finland, University of Helsinki. Sequencing was performed by DOE JGI and the sequencing project was a part of the DOE JGI 2014 Genomic Encyclopedia of Type Strains, Phase III the genomes of soil and plant-associated and newly described type strains. We would like to thank Professor J.P.W. Young (University of York, UK) for providing Conting fasta files of additional reference genome reads. The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, was supported under Contract No. DE-AC02-05CH11231.
AAA, KL and WBW planned the genome sequencing project. AAA isolated the described strains and performed cultivation, microbiological laboratory experiments, phenotypic characterization, DNA extraction, PCR, 16S rRNA gene, recA and glnII gene sequences analyses. AAA prepared phylogenetic trees, figures, genomic data analysis and wrote the manuscript. TW and NCK participated in the genome sequencing, assembly and genome annotation. KL and WBW conceived of the study and participated in its design and coordination. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Phenotypic characteristics of Rhizobium aethiopicum sp. nov. strains. (DOCX 21 kb)
Carbon sources utilization response between Rhizobium aethiopicum sp. nov. strains and Rhizobium etli CFN42T. (DOCX 25 kb)
Rhizobium aethiopicum sp.nov. strain HBR26T repABC and symbiotic genes homologous to genes found in the symbiotic plasmid 42d of Rhizobium etli CFN 42T. (XLSX 45 kb)