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Draft genome sequence of Halopiger salifodinae KCY07-B2T, an extremly halophilic archaeon isolated from a salt mine

Standards in Genomic Sciences volume 10, Article number: 124 (2015) Cite this article

Abstract

Halopiger salifodinae strain KCY07-B2T, isolated from a salt mine in Kuche county, Xinjiang province, China, belongs to the family Halobacteriaceae. It is a strictly aerobic, pleomorphic, rod-shaped, Gram-negative and extremely halophilic archaeon. In this work, we report the features of the type strain KCY07-B2T, together with the draft genome sequence and annotation. The draft genome sequence is composed of 83 contigs for 4,350,718 bp with 65.41 % G + C content and contains 4204 protein-coding genes and 50 rRNA genes.

Introduction

The genus Halopiger , which belongs to the family Halobacteriaceae , was originally established in 2007 by Gutiérrez et al. [1]. The type species of the genus Halopiger is Halopiger xanaduensis SH-6T. To date, the genus is comprised of three validly published species and two effectively but not validly published species: H. xanaduensis [1], Halopiger aswanensis [2], Halopiger salifodinae [3], Halopiger djelfamassiliensis [4] and Halopiger goleamassiliensis [5]. The species of the genus were reported to be isolated from hypersaline environments such as salt lake sediment [1, 4, 5], hypersaline soil [2] and salt mine [3]. All are Gram-negative, strictly aerobic and extremely halophilic [1–5]. In this genus, three genome sequences, including one finished genome sequence H. xanaduensis SH-6T, and two draft genome sequences H. djelfamassiliensis IIH2T and H. goleamassiliensis IIH3T, are available in Standards in Genomic Sciences [4–6], except H. aswanensis 56T which showed highest 16S rRNA gene similarity to H. xanaduensis SH-6T (99.1 %). Here we present a summary of the classification and a set of features of strain H. salifodinae KCY07-B2T, together with a description of the non-contiguous finished genomic sequencing and annotation.

Organism Information

Classification and features

A representative genomic 16S rRNA gene sequence of H. salifodinae KCY07-B2T was compared with sequences deposited in the GenBank database using BLASTN [7]. The 16S rRNA gene sequence analysis showed that H. salifodinae KCY07-B2T shared the highest sequence identities to H. xanaduensis SH-6T (95.8 %), followed by H. aswanensis 56T (95.5 %), H. djelfamassiliensis IIH2T (94.9 %) and H. goleamassiliensis IIH3T (94.8 %), and shared low sequence similarities (<94.8 %) to species of other genera. The phylogenetic tree was reconstructed by the neighbor-joining method using MEGA 5 and Kimura’s 2-parameter model for distance calculation [8, 9]. The phylogenetic tree was assessed by boot-strapping for 1000 replications, and the consensus tree was shown in Fig. 1.

Fig. 1
figure 1

Neighbor-joining phylogenetic tree based on 16S rRNA gene sequences showed the relationship of H. salifodinae KCY07-B2T and other related haloarchaeal species. GenBank accession numbers were indicated in parentheses. Bootstrap values based on 1000 replicates were shown for branches with more than 60 % support. Bar, 0.02 substitutions per nucleotide positions. Methanospirillum hungatei JF-1T [30] was used as outgroup

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H. salifodinae KCY07-B2T can tolerant high salinity (5.4 M NaCl ) and high temperature (50 °C) [3]. Cells lyse in distilled water. The optimal growth condition of strain KCY07-B2T occured in medium NOM-3 with 2.9–3.4 M NaCl [3]. The optimum temperature was 37–45 °C. The optimum pH was 7.0, with a growth range of pH 6.0–8.0 [3]. Cells of strain KCY07-B2T are strictly aerobic, non-motile and pleomorphic rod-shaped (Fig. 2). Several sugars, organic acids and amino acids can serve as sole carbon and energy sources, and amino acids are not required in the growth medium [3]. The features of H. salifodinae KCY07-B2T are listed in Table 1.

Fig. 2
figure 2

Electron micrographs of cells of strain KCY07-B2T grown in liquid medium under optimum condition. a Transmission electron micrographs of strain KCY07-B2T revealing rod-shaped, bar, 0.2 μm, b Showing a mixture of pleomorphic cells including short and long rod-shaped, bar, 2 μm

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Table 1 Classification and general features of H. salifodinae KCY07-B2T according to the MIGS recommendations [10]
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Genome sequencing information

Genome project history

This genome was selected for sequencing on the basis of its phylogenetic position and 16S rRNA sequence similarity to other members of the genus Halopiger . This whole genome shotgun project of strain H. salifodinae KCY07-B2T was deposited at DDBJ/EMBL/GenBank under the accession number JROF00000000 and the sequence consisted of 83 contigs (further assembling constructed these contigs into 81 scaffolds). Table 2 shows the project information and its association with MIGS version 2.0 compliance [10].

Table 2 Project information
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Growth conditions and genomic DNA preparation

H. salifodinae KCY07-B2T was cultivated aerobically on 37 °C for 4 days in NOM-3 medium, which contains (per liter distilled water) 5.4 g KCl, 0.3 g K2HPO4, 0.25 g CaCl2, 0.25 g NH4Cl, 26.8 g MgSO4 · 7H2O, 23.0 g MgCl2 · 6H2O, 184.0 g NaCl, 1.0 g yeast extract, 0.25 g fish peptone, 0.25 g sodium formate, 0.25 g sodium acetate, 0.25 g sodium lactate and 0.25 g sodium pyruvate (adjusted to pH 7.0 with 1 M NaOH) [3]. Genomic DNA was extracted using the method described by Marmur [11]. The purity, quality and the concentration of genomic DNA preparation were analyzed by 0.7 % agarose gel electrophoresis with λ-Hind III digest DNA Marker (TaKaRa, Dalian, China) and measured using a NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific Inc., USA).

Genome sequencing and assembly

The genome of H. salifodinae KCY07-B2T was sequenced using Solexa paired-end sequencing technology (HiSeq2000 system, Illumina, Inc., USA) [12]. A shotgun library was constructed with a 500 bp-span paired-end library (~500 Mb available reads, ~130-fold genome coverage) and a 2000 bp-span paired-end library (~250 Mb available reads, ~65-fold genome coverage). The sequence data from an Illumina HiSeq 2000 were assembled with SOAPdenovo v.1.05 [13–15]. The final assembly identified 83 contigs and 81 scaffolds (the minimum length is 523 bp) generating a genome size of 4.35 Mb. The quality of the sequencing reads data was estimated by G + C content and sequencing depth correlation analysis.

Genome annotation

The tRNAs and rRNAs were identified using tRNAscan-SE [16], RNAmmer [17] and Rfam database [18]; The open reading frames and the functional annotation of translated ORFs were predicted and achieved by using the RAST server online [19, 20]. Classification of some predicted genes and pathways were analyzed using COGs [21, 22] and KEGG [23–25] databases. Meanwhile, we used CRISPRs web server [26] to predict CRISPRs and InterPro [27, 28] to obtain the GO annotation with the database of Pfam [29].

To estimate the mean level of nucleotide sequence similarity at the genome level between e KCY07-B2T and the genus Halopier genomes available to date ( H. xanaduensis SH-6T, H. djelfamassiliensis IIH2T and H. goleamassiliensis IIH3T), we compared the ORFs only using comparison sequence based in the server RAST [19] at a query coverage of ≥60 % and a minimum nucleotide length of 100 bp.

Genome properties

The draft genome sequence of H. salifodinae KCY07-B2T revealed a genome size of 4,350,718 bp (scaffold length) with a 65.41 % G + C content. Of the 4254 predicted genes, 4204 were protein-coding genes, and 50 were rRNA genes. There were one 16S rRNA gene, two 23S rRNA genes and two 5S rRNA genes. A total of 2887 genes (68.67 %) were assigned a putative function (Table 3). Table 4 showed the distribution of genes into COG functional categories.

Table 3 Genome statistics of Halopiger salifodinae KCY07-B2T, including nucleotide content and gene count levels
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Table 4 Number of genes associated with the 25 general COG functional categories
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Insights from the genome sequence

Strain H. salifodinae KCY07-B2T was isolated from a salt mine sample. The experiments showed this strain could grow at 2.9–3.4 M NaCl for optimal growth, and the cells lysed in distilled water. So the analysis of the genome sequence focused on the adaption mechanism of the halophilic archaea in hypersaline-environments. Strain H. salifodinae KCY07-B2T mainly utilized “the salt-in strategy” to maintain osmotic balance. According to the annotation of genome sequence, Trk system potassium uptake protein were found, which were responsible for K+ uptake and transport, including 9 copies TrkH genes and 5 copies TrkA genes. Five copies of Kef-type K+ transport proteins, one copy glutathione-regulated potassium-efflux protein KefB and 8 pH adaptation potassium efflux system proteins were found that were related to K+ efflux. And there also existed 8 copies of potassium channel proteins. In addition, the genome contains 13 copies of Na+/ H+ antiporter proteins related to Na+ efflux. The genome of strain H. salifodinae KCY07-B2T contains 12 genes related to the synthesis and transport of the compatible-solute glycine betaine for resistance to osmotic stress including: 7 choline-sulfatases, 2 high-affinity choline uptake protein BetTs, 2 glucose-methanol-choline oxidoreductase and 1 glycine betaine transporter OpuD coding genes. These proteins were also related to the metabolic pathway converting choline sulfate to glycine betaine. All these proteins and systems mentioned played an important role in the adaption of osmotic stress in high salt environment.

Currently, three genomes from Halopiger species are available. Here, we compare the genome of strain H. salifodinae KCY07-B2T with strains H. xanaduensis SH-6T, H. djelfamassiliensis IIH2T and H. goleamassiliensis IIH3T (Table 5). The size of genome of H. salifodinae KCY07-B2T (4.35 Mb) is similar to H. xanaduensis SH-6T (4.35 Mb) but larger than that of H. djelfamassiliensis IIH2T (3.77 Mb) and H. goleamassiliensis IIH3T (3.90 Mb). The G + C content of H. salifodinae KCY07-B2T (65.41 %) is similar to H. xanaduensis SH-6T (65.18 %) and higher than that of H. djelfamassiliensis IIH2T (64.30 %) but lower than that of H. goleamassiliensis IIH3T (66.06 %). In addition, H. salifodinae KCY07-B2T shares a mean genomic sequence similarity of 79.74 %, 80.16 % and 79.17 % with strains H. xanaduensis SH-6T, H. djelfamassiliensis IIH2T and H. goleamassiliensis IIH3T, respectively.

Table 5 Genomic comparison of H. salifodinae KCY07-B2T with three other Halopiger speciesa
Full size table

Conclusions

Strain KCY07-B2T is the third member of the genus Halopiger to be described and the fourth whose genome sequence report is available. These data will provide a new perspective of how microorganisms adapt to halophilic environments, and may also provide a pool of functional enzymes that work at higher salty.

Abbreviations

NCBI:

National Center for Biotechnology Information

EMBL:

European Molecular Biology Laboratory

DDBJ:

DNA Data Bank of Japan

BLASTN:

Basic Local Alignment Search Tool for Nucleotide

MIGS:

Minimum Information about a Genome Sequence

RAST:

Rapid Annotations using Subsystems Technology

COG:

Cluster of Orthologous Groups of proteins

KEGG:

Kyoto Encyclopedia of Genes and Genomes

CRISPR:

Clustered Regularly Interspaced Short Palindromic repeat sequences

GO:

Gene Ontology

DNA:

Deoxyribonucleic Acid

16S rRNA:

ribosomal Ribonucleic Acid

JCM:

Japan Collection of Microorganisms

CGMCC:

China General Microbiological Culture Collection Center

H. salifodinae KCY07-B2T :

Halopiger salifodinae KCY07-B2T

H. xanaduensis SH-6T :

Halopiger xanaduensis SH-6T

H. aswanensis 56T :

Halopiger aswanensis 56T

H. djelfamassiliensis IIH2T :

Halopiger djelfamassiliensis IIH2T

H. goleamassiliensis IIH3T :

Halopiger goleamassiliensis IIH3T

References

  1. Gutiérrez MC, Castillo AM, Kamekura M, Xue Y, Ma Y, Cowan DA, et al. Halopiger xanaduensis gen. nov., sp. nov., an extremely halophilic archaeon isolated from saline Lake Shangmatala in Inner Mongolia, China. Int J Syst Evol Microbiol. 2007;57:1402–7.

    Article  PubMed  Google Scholar 

  2. Hezayen FF, Gutiérrez MC, Steinbüchel A, Tindall BJ, Rehm BH. Halopiger aswanensis sp. nov., a polymer-producing and extremely halophilic archaeon isolated from hypersaline soil. Int J Syst Evol Microbiol. 2010;60:633–7.

    Article  CAS  PubMed  Google Scholar 

  3. Zhang WY, Meng Y, Zhu XF, Wu M. Halopiger salifodinae sp. nov., an extremely halophilic archaeon isolated from a salt mine. Int J Syst Evol Microbiol. 2013;63:3563–7.

    Article  CAS  PubMed  Google Scholar 

  4. Hassani II, Robert C, Michelle C, Raoult D, Hacène H, Desnues C. Non-contiguous finished genome sequence and description of Halopiger dielfamassiliensis sp. nov. Stand Genomic Sci. 2013;9:160–74.

    Article  PubMed Central  PubMed  Google Scholar 

  5. Hassani II, Robert C, Michelle C, Raoult D, Hacène H, Desnues C. Non-contiguous finished genome sequence and description of Halopiger goleamassiliensis sp. nov. Stand Genomic Sci. 2014;9:956–69.

    Google Scholar 

  6. Anderson I, Tindall BJ, Rohde M, Lucas S, Han J, Lapidus A, et al. Complete genome sequence of Halopiger xanaduensis type strain (SH-6T). Stand Genomic Sci. 2012;6:31–42.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  7. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990;215:403–10.

    Article  CAS  PubMed  Google Scholar 

  8. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4:406–25.

    CAS  PubMed  Google Scholar 

  9. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol Biol Evol. 2011;28:2731–9.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  10. Field D, Garrity G, Gray T, Morrison N, Selengut J, Sterk P, et al. The minimum information about a genome sequence (MIGS) specification. Nat Biotechnol. 2008;26:541–7.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  11. Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962;5:109–18.

    Article  CAS  PubMed  Google Scholar 

  12. Bentley DR, Balasubramanian S, Swerdlow HP, Smith GP, Milton J, Brown CG, et al. Accurate whole human genome sequencing using reversible terminator chemistry. Nature. 2008;456:53–9.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  13. Li R, Zhu H, Ruan J, Qian W, Fang X, Shi Z, et al. De novo assembly of human genomes with massively parallel short read sequencing. Genome Res. 2010;20:265–72.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  14. Li R, Li Y, Kristiansen K, Wang J. SOAP: short oligonucleotide alignment program. Bioinformatics. 2008;24:713.

    Article  CAS  PubMed  Google Scholar 

  15. SOAP. denovo v.1.05. http://soap.genomics.org.cn/soapdenovo.html.

  16. Lowe TM, Eddy S. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res. 1997;25:955–64.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  17. Lagesen K, Hallin P, Rdland EA, Strfeldt HH, Rognes T, Ussery DW. RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res. 2007;35:3100.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  18. Griffiths-Jones S, Bateman A, Marshall M, Khanna A, Eddy SR. Rfam: an RNA family database. Nucleic Acids Res. 2003;31:439.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  19. Aziz RK, Bartels D, Best A, DeJongh M, Disz T, Edwards R, et al. Server: rapid annotations using subsystems technology. BMC Genomics. 2008;9:75–89.

    Article  PubMed Central  PubMed  Google Scholar 

  20. RAST server online. http://rast.nmpdr.org/.

  21. Tatusov RL, Natale D, Garkavtsev I, Tatusova T, Shankavaram U, Rao B, et al. The COG database: new developments in phylogenetic classification of proteins from complete genomes. Nucleic Acids Res. 2001;29:22–8.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  22. Tatusov RL, Galperin MY, Natale DA, Koonin EV. The COG database: a tool for genome-scale analysis of protein functions and evolution. Nucleic Acids Res. 2000;28:33–6.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  23. Kanehisa M, Araki M, Goto S, Hattori M, Hirakawa M, Itoh M, et al. KEGG for linking genomes to life and the environment. Nucleic Acids Res. 2008;36(Database issue):D480–4.

    PubMed Central  CAS  PubMed  Google Scholar 

  24. Moriya Y, Itoh M, Okuda S, Kanehisa M. KAAS: KEGG automatic annotation server. Genome Informatics. 2005;5:2005.

    Google Scholar 

  25. Kanehisa M, Goto S. KEGG: Kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 2000;28:27.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  26. Grissa I, Vergnaud G, Pourcel C. CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats. Nucleic Acids Res. 2007;35:52–7.

    Article  Google Scholar 

  27. Zdobnov EM, Apweiler R. InterProScan-an integration platform for the signature-recognition methods in InterPro. Bioinformatics. 2001;17:847.

    Article  CAS  PubMed  Google Scholar 

  28. Apweiler R, Attwood TK, Bairoch A, Bateman A, Birney E, Biswas M, et al. The InterPro database, an integrated documentation resource for protein families, domains and functional sites. Nucleic Acids Res. 2001;29:37.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  29. Bateman A, Coin L, Durbin R, Finn RD, Hollich V, Griffiths Jones S, et al. The Pfam protein families database. Nucleic Acids Res. 2004;32 suppl 1:138D.

    Article  Google Scholar 

  30. Ferry JG, Smith PH, Wolfe RS. Methanospirillum, a new genus of methanogenic bacteria, and characterization of Methanospirillum hungatii sp. nov. Int J Syst Bacteriol. 1974;24:465–9.

    Article  CAS  Google Scholar 

  31. Woese CR, Kandler O, Wheelis ML. Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya. Proc Natl Acad Sci U S A. 1990;87:4576–9.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  32. Garrity GM, Holt JG. Phylum AII. Euryarchaeota phy. nov. In: Garrity GM, Boone DR, Castenholz RW (eds), Bergey’s Manual of Systematic Bacteriology, Second Edition, Springer. N Y. 2001;1:211–355.

    Google Scholar 

  33. Validation List no. 85. Validation of publication of new names and new combinations previously effectively published outside the IJSEM. Int J Syst Evol Microbiol. 2002; 52: 685–90.

  34. Grant WD, Kamekura M, McGenity TJ, Ventosa A. Class III. Halobacteria class. nov. In: Garrity GM, Boone DR, Castenholz RW, editors. Bergey’s Manual of Systematic Bacteriology. 2nd ed. New York: Springer; 2001. p. 294.

    Google Scholar 

  35. Grant WD, Larsen H. Group III. Extremely halophilic archaebacteria, Order Halobacteriales ord. nov. In: Holt JG, editor. Bergey’s Manual of Systematic Bacteriology, vol. 3. Baltimore: Williams & Wilkins; 1989. p. 2216–28.

    Google Scholar 

  36. Validation List no. 31. Validation of the publication of new names and new combinations previously effectively published outside the IJSB. Int J Syst Bacteriol. 1989; 39:495–97.

  37. Judicial Commission of the International Committee on Systematics of Prokaryotes. The nomenclatural types of the orders Acholeplasmatales, Halanaerobiales, Halobacteriales, Methanobacteriales, Methanococcales, Methanomicrobiales, Planctomycetales, Prochlorales, Sulfolobales, Thermococcales, Thermoproteales and Verrucomicrobiales are the genera Acholeplasma, Halanaerobium, Halobacterium, Methanobacterium, Methanococcus, Methanomicrobium, Planctomyces, Prochloron, Sulfolobus, Thermococcus, Thermoproteus and Verrucomicrobium, respectively. Opinion 79. Int J Syst Evol Microbiol. 2005;55:517–8.

    Article  Google Scholar 

  38. Gibbons NE, Family V. Halobacteriaceae fam. nov. In: Buchanan RE, Gibbons NE, editors. Bergey’s Manual of Determinative Bacteriology. 8th ed. Baltimore: The Williams and Wilkins Co.; 1974. p. 269–73.

    Google Scholar 

  39. Skerman VBD, McGowan V, Sneath PHA. Approved Lists of Bacterial Names. Int J Syst Bacteriol. 1980;30:225–420.

    Article  Google Scholar 

  40. Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, et al. Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet. 2000;25:25–9.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  41. Gene Ontology project. http://geneontology.org/

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Acknowledgements

We thank Hong Cheng for her help on offering some websites for data analysis. This work was supported by the China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (grant no. DY125-14-E-02) and the Chinese Natural Science Foundation (grant no. 31170001).

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Authors and Affiliations

  1. College of Life Sciences, Zhejiang University, Hangzhou, 310058, P. R. China

    Wei-Yan Zhang, Jing Hu, Jie Pan, Cong Sun & Min Wu

  2. Second Institute of Oceanography, State Oceanic Administration, Hangzhou, 310012, P. R. China

    Xue-Wei Xu

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Correspondence to Min Wu or Xue-Wei Xu.

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The authors declare that they have no competing interests.

Authors’ contributions

WYZ designed the study, isolated strain Halopiger salifodinae KCY07-B2T, performed the laboratory experiments, analyzed the genome and wrote the manuscript. JH worked on genome assembly, annotated the genome and discussed the results. JP and CS participated in the analysis of the genome and checked the manuscript. MU and XWX helped to supervise the study and revised the manuscript. All authors read and approved the final manuscript.

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Zhang, WY., Hu, J., Pan, J. et al. Draft genome sequence of Halopiger salifodinae KCY07-B2T, an extremly halophilic archaeon isolated from a salt mine. Stand in Genomic Sci 10, 124 (2015). https://doi.org/10.1186/s40793-015-0113-y

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Keywords

Environmental Microbiome

ISSN: 2524-6372