Table 8 Enzymes involved in lignin degradation with homologues in BRL6-1^T genome

Cα-dehydrogenase involved in b-aryl ether cleavage

ligD

Sphingomonas paucimobilis SYK-6

2

6e-12; 4e-10

1)3-oxoacyl-[acyl-carrier-protein] reductase (EC 1.1.1.100)

2)hypothetical protein

Beta-etherase

ligF

Sphingomonas paucimobilis SYK-6

1

4e-11

Glutathione S-transferase

Feruloyl-CoA synthetase

fcs

Pseudomonas putida strain KT2440

1

1e-10

Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Feruloyl-CoA hydratase/lyase

ferB

Sphingomonas paucimobilis

1

7e-15

1,4-Dihydroxy-2-naphthoyl-CoA synthase (EC 4.1.3.36)

Feruloyl-CoA hydratase/lyase

ferB2

Sphingomonas paucimobilis

1

3e-32

1,4-Dihydroxy-2-naphthoyl-CoA synthase (EC 4.1.3.36)

Vanillin dehydrogenase

ligV

Sphingomonas paucimobilis

5

4e-66; 3e-65; 2e-62; 2e-35; 8e-08

1) succinate semialdehyde dehydrogenase (EC 1.2.1.16)

2) gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase

3) NAD-dependent aldehyde dehydrogenases

4) succinylglutamic semialdehyde dehydrogenase (EC 1.2.1.71)

5) acetaldehyde dehydrogenase (EC 1.2.1.10)/alcohol dehydrogenase AdhE (EC 1.1.1.1)

Vanillate O-demethylase oxidoreductase

vanB

Pseudomonas syringae pv. tomato strain DC3000

3

9e-18; 7e-14; 9e-12

1)Hemoglobin-like flavoprotein

2)Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

3)Predicted ferric reductase

3-carboxymuconate cycloisomerase

pcaB

Pseudomonas putida

2

9e-13; 5e-09

1)Adenylosuccinate lyase (EC 4.3.2.2)

2)argininosuccinate lyase (EC 4.3.2.1)

4-carboxymuconolactone decarboxylase

pcaC

Pediococcus acidilactici D3

3

6e-30; 7e-27; 1e-06

Uncharacterized homolog of gamma-carboxymuconolactone decarboxylase subunit (all 3)

Β-ketoadipate enol-lactone hydrolase

pcaD

Acinetobacter baumannii strain SDF

2

5e-15; 5e-13

1)carboxylesterase BioH (pimeloyl-CoA synthesis) (EC 3.1.1.85)

2)Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)