From: The JCVI standard operating procedure for annotating prokaryotic metagenomic shotgun sequencing data
BLAST Hit Class | Annotative meaning |
---|---|
High Confidence | 35% identity or greater, across 85% or more of the length |
Putative | less than 35% identity, but across 80% or more of the length |
Conserved Domain | 35% identity or greater, but across less than 80% of the length |
Low Confidence | less than 35% identity across less than 80% of the length |