Figure 1.

Phylogenetic tree highlighting the position of S. cyanea relative to the type strains of the other species within the family Pseudonocardiaceae. The tree was inferred from 1,371 aligned characters [16,17] of the 16S rRNA gene sequence under the maximum likelihood (ML) criterion [18]. Rooting was done initially using the midpoint method [19] and then checked for its agreement with the current classification (Table 1). The branches are scaled in terms of the expected number of substitutions per site. Numbers adjacent to the branches are support values from 600 ML bootstrap replicates [20] (left) and from 1,000 maximum-parsimony bootstrap replicates [21] (right) if larger than 60%. Lineages with type strain genome sequencing projects registered in GOLD [22] are labeled with one asterisk, those also listed as ‘Complete and Published’ with two asterisks [4,23,24] (S. azurea [25] and S. marina [26] miss their second asterisk due to very recent publication). Actinopolyspora iraqiensis Ruan et al. 1994 was ignored in the tree, because a proposal for the transfer of this species to the genus Saccharomonospora [27] was recently rejected on formal criteria [3].