Fig. 4

Optimized metagenomic workflow. We have created an optimized MetaG workflow to improve the quality of the MAGs. This is achieved by assembly and binning of the reads individually, in parallel to a co-assembly, and combined and dereplicated to exclude redundant MAGs before bin annotation, gene prediction and functional annotation. Two samples S1 and S2 are shown as an example. Differences to the original MetaG workflow are highlighted in yellow