What matters most? Assessment of within-canopy factors influencing the needle microbiome of the model conifer, Pinus radiata

Table 2 Summary PERMANOVA testing for the effects of main and interaction sampling effects on the composition of bacteria and fungal communities in the Pinus radiata phyllosphere

	Bacteria¹		Fungi
Test	√CV	ρ	√CV	ρ
Canopy height	19.20	0.001	21.42	0.001
Cardinal direction	14.48	0.001	-2.88	0.610
Needle age	4.94	0.064	-3.33	0.730
Surface sterilisation	4.20	0.075	6.75	0.025
Height of sampling x cardinal direction	19.90	0.006	4.42	0.276
Height of sampling x needle age	18.32	0.008	3.99	0.305
Height of sampling x surface sterilisation	9.07	0.062	-2.21	0.465
Cardinal direction x needle age	5.82	0.222	-6.07	0.823
Cardinal direction x surface sterilisation	5.98	0.147	-5.08	0.746
Needle age x surface sterilisation	3.34	0.234	-3.53	0.569
Residual	77.04		26.09

¹ Bacteria are samples from the middle and bottom canopy levels only. Top canopy samples were excluded
√CV = square root of the component of variation associated for each term
ρ values are the permutation-derived probability statistic. Unique permutations for all tests > 998

ISSN: 2524-6372