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Fig. 5 | Environmental Microbiome

Fig. 5

From: Sequencing introduced false positive rare taxa lead to biased microbial community diversity, assembly, and interaction interpretation in amplicon studies

Fig. 5

Cow rumen microbial interaction network revealed by DNBSEQ and NovaSeq. A The cooccurrence network of microbial communities in cow rumen. Each node represents an OTU and was color coded by both sub-community type and sequencing platform where appropriate. The size of the node is proportional to its node degree. Each line represents a potential correlation interaction, with blue lines indicating positive interaction while green lines indicating negative interaction. Only interactions with a correlation coefficient greater than 0.75 and significance of P smaller than 0.05 were plotted. B Node degree distribution and the Natural Connectivity change under node attack test of the microbial interaction network revealed by DNBSEQ and NovaSeq platforms. C Phylogeny of the top 20 nodes with the highest degree in the microbial interaction networks revealed by DNBSEQ and NovaSeq. The size of the solid circle is proportional to its node degree and color coded by the sub-community type. Solid or open asterisk indicates the platform by which the OTUs were identified as the top 20 nodes with the highest degree

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