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Fig. 4 | Environmental Microbiome

Fig. 4

From: Sequencing introduced false positive rare taxa lead to biased microbial community diversity, assembly, and interaction interpretation in amplicon studies

Fig. 4

Cow rumen microbial community assembly mechanism. A The fit of Sloan’s neutral model to the cow rumen microbial communities revealed by each sequencing platform. The proportion of OTUs distributed within the Sloan’s neutral model, at the upper or lower part were indicated. R2 values indicate the overall fit of the model, and m values indicate the estimated migration rate. Dashed lines represent 95% confidence intervals around the model prediction. B Bar charts shows the OTUs’ frequency distribution on each sequencing platform. More singleton OTUs and low frequent OTUs were observed on NovaSeq sequencing platform, which was largely driven by the OTUs specifically detected by NovaSeq platform. C The distribution of βNTI values between cow rumen microbial communities revealed by each sequencing platform. Each point represents a βNTI value. A |βNTI| value of less than 2 (grey shaded region) indicates stochastic assembly processes; a βNTI value of less than − 2 indicates a homogeneous selection event; and a βNTI value of greater than 2 indicates a heterogeneous selection event

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Environmental Microbiome

ISSN: 2524-6372