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Fig. 3 | Environmental Microbiome

Fig. 3

From: Sequencing introduced false positive rare taxa lead to biased microbial community diversity, assembly, and interaction interpretation in amplicon studies

Fig. 3

Characteristics of cow rumen microbial communities revealed by different sequencing platforms A Number of shared abundant, moderate, and rare unique OTUs detected by different sequencing platforms in cow rumen. B Comparison of Chao I index, Shannon index, and the phylogenetic diversity of the overall community, abundant sub-community, moderate sub-community, and rare sub-community as revealed by DNBSEQ and NovaSeq sequencing platforms in cow rumen. C Phylogenetic distribution of OTUs detected by both or either of the DNBSEQ or NovaSeq sequencing platforms in cow rumen. The inner circle was color coded by phylogeny, and the outer circle was color coded according to whether the OTU was consistently detected by both sequencing platforms, or specifically detected by either of DNBSEQ or NovaSeq sequencing platform. Heatmap in the middle panel shows the relative abundance of different phylum revealed by DNBSEQ or NovaSeq. Bar chart on the right panel shows the number of unique OTUs specifically detected by NovaSeq platform in different phylum

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