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Fig. 2 | Environmental Microbiome

Fig. 2

From: Sequencing introduced false positive rare taxa lead to biased microbial community diversity, assembly, and interaction interpretation in amplicon studies

Fig. 2

Comparison of the amplicon sequencing results of three typical ecological systems between DNBSEQ and NovaSeq sequencing platforms. A Evaluation of the reproducibility of the amplicon sequencing results of DNBSEQ and NovaSeq sequencing platform in revealing the membership of microbes of abundant, moderate and rate taxa subcommunity from ecosystems with various complexity (DNB All, DNB 2, DNB 3 denotes number of all unique OTUs detected by DNBSEQ platform, consistently detected by at least 2 technical replicates, and consistently detected by all three technical replicates, respectively. Similar naming scheme was used for the NovaSeq platform). B/C Comparison of the alpha diversity of the overall community, abundant sub-community, moderate sub-community, and rare sub-community as revealed by DNBSEQ and NovaSeq sequencing platforms based on Observed OTU Number B and Chao I index C

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Environmental Microbiome

ISSN: 2524-6372