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Fig. 1 | Environmental Microbiome

Fig. 1

From: Sequencing introduced false positive rare taxa lead to biased microbial community diversity, assembly, and interaction interpretation in amplicon studies

Fig. 1

Amplicon sequencing of the commercial mock community. A The number of shared OTUs among technical triplicates for both DNBSEQ and Novaseq platforms, and the number of shared unique OTUs detected by pooled technical triplicates by different sequencing platforms and the theoretical members of the mock community. B Sankey diagram showing the phylogeny and number of unique OTUs detected by each sequencing platform. For the expected OTUs, the height of the bars was proportional to the averaged relative abundance reported by two sequencing platforms. OTUs detected by both DNBSEQ and NovaSeq, or specifically detected by NovaSeq platform were indicated and color coded. C Phylogenetic tree of the expected members of the microbes and OTUs detected by both sequencing platforms

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Environmental Microbiome

ISSN: 2524-6372