Methanol utilizers of the rhizosphere and phyllosphere of a common grass and forb host species

Table 2 Methanol dehydrogenase (MDH) genes in methanol utilizers from MAGs

Species level	Class level	MDH gene	Percent identity	Method
Methylosinus trichosporium	Alphaproteobacteria	xoxF	98%	Phylogenetic analysis (maximum likelihood)
Cohnella sp.	Bacilli	PQQ-dependent	95%	RAST/protein BLAST
Uncultured Actinobacteria sp.	Actinobacteria	PQQ-dependent	98%	RAST/protein BLAST
Uncultured Acidobacteria sp.	Acidobacteria	PQQ-dependent	94%	RAST/protein BLAST
Methylobacillus flagellatus	Betaproteobacteria	mxaF	100%	Phylogenetic analysis (maximum likelihood)
Uncultured Gammaproteobacteria sp.	Gammaproteobacteria	PQQ-dependent	95%	RAST/protein BLAST
Methylooceanibacter Superfactus	Alphaproteobacteria	xoxF1	92%	Phylogenetic analysis (maximum likelihood)
Uncultured Deltaproteobacteria sp.	Deltaproteobacteria	PQQ-dependent (MoxR gene)	96%	RAST/protein BLAST
Gemmatimonas aurantiaca	Gemmatimonadetes	PQQ-dependent	98%	RAST/protein BLAST
Verminephrobacter eiseniae	Betaproteobacteria	xoxF5	68%	Phylogenetic analysis (maximum likelihood)
Candidatus Methylomirabilis oxyfera	Candidate division NC10	xoxF1	90%	Phylogenetic analysis (maximum likelihood)
Salinispora arenicola	Actinobacteria	PQQ-dependent	95%	RAST/protein BLAST

RAST-annotated bins are listed at the species and class levels. They were screened for the presence of MDH genes using three different methods, namely, RAST, protein BLAST (NCBI) and phylogenetic analysis (MEGA7). Percent identity with respect to MDH genes was also obtained using the abovementioned methods

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