Fig. 4

Distribution of genome sizes of Patescibacteria MAGs enriched in 0.1 µm and 0.2 µm filter fractions. A For all 291 high-quality Patescibacteria MAGs, the ratio of average normalized genome coverage in 0.1 µm filter fractions to 0.2 µm filter fractions from metagenomes was used to form three groups: ‘0.1 µm filter’—MAGs where this ratio was at least 5, ‘0.2 µm filter’—MAGs where this ratio was 1/5 or less, and ‘None’—MAGs other than first two groups. The mean genome sizes were significantly different (Kruskal–Wallis rank sum test, p = 2.24e−06). Pairwise Dunn’s test showed the genome sizes were significantly different between ‘0.1 µm filter’ and ‘0.2 µm filter’ (fdr adjusted p = 1.02e−06), and between ‘0.2 µm filter’ and ‘None’ (fdr adjusted p = 9.08e−05). B The scatter plot shows the distribution of log₂ filter enrichment factors (the ratio of average normalized genome coverage in 0.2 µm filter fractions to 0.1 µm filter fractions from metagenomes) of Patescibacteria MAGs, as the function of their genome sizes. The dashed lines indicate the cut-off value of 5 and 1/5 for filter enrichment factors on the y-axis