Fig. 6

Demonstration of threshold functionality of BLAST-QC. Command 1 demonstrates the usage of BLAST-QC to return the top e-value hit per query, while command 2 depicts the usage of BLAST-QC to return the top e-value hit per query with a bitscore threshold of 60 (−b 60). The results of command 1 return a hit with an e-value of .0015, while the hit’s bitscore is only 32.40. the results of command 2 return a hit with an e-value of .005, but with a bitscore of 66.5