Table 1 Overview of the three DNA isolation methods evaluated in this work
Method | MetaSUB | Jiang | Zymobiomics |
|---|---|---|---|
Common processing steps (used to generate equal aerosol biomass aliquots for paired difference comparison) | |||
Filter extraction (filter-to-liquid) | NucliSENS lysis buffer | ||
Lysis (enzymatic) | MetaPolyzyme multi-enzyme cocktail | ||
Method-specific processing steps (used for paired difference comparison on equal aerosol biomass aliquots) | |||
Lysis (mechanical) | ZR BashingBead Tubes with PowerSoil Bead Solution and Solution C1. Bead beating for 3 min | PowerSoil Bead Tubes with PowerSoil Bead Solution and Solution C1 incubated at 65 °C for 15 min. Bead vortexing for 15 min | ZR BashingBead Tubes with Zymobiomics lysis solution. Bead beating for 3 min |
Inhibitor removal and sample clean-up | PowerSoil Solution C2 and C3 | PowerSoil Solution C2 and C3 | Zymo-Spin IV and Zymo-Spin IV-μHRC Columns |
DNA purification | NucliSENS magnetic beads | AMPure XP magnetic beads | Zymo-Spin IC-Z Column |