Fig. 1From: Draft genome sequence of Acidithiobacillus thiooxidans CLST isolated from the acidic hypersaline Gorbea salt flat in northern ChilePhylogenetic tree based on the 16S rRNA gene sequences highlighting the position of Acidithiobacillus thiooxidans strain CLST relative to other type and non-type strains of the genus Acidithiobacillus . The GenBank database accession codes are indicated between brackets. The evolutionary history was inferred by using the Maximum Parsimony and the Subtree-Pruning-Regrafting (SPR) algorithm with search level 1 [52]. The initial trees were obtained by the random addition of sequences. The analysis involved 16 nucleotide sequences and a total of 1307 non-ambiguous positions in the final dataset. Evolutionary analyses were conducted in MEGA version 6.22 [53]. Tree construction used a bootstrapping process repeated 1000 times to generate a majority consensus tree. A sequence from Thermithiobacillus tepidarius was used as outgroup. The tree is drawn to scale, with branch lengths calculated using the average pathway method [53]; the scale bar corresponds to the number of changes over the whole sequenceBack to article page