Table 5 Putative hyaluromycin biosynthetic gene cluster and the neighboring genes
From: Draft genome sequence of Streptomyces hyaluromycini MB-PO13T, a hyaluromycin producer
Orf1- | Size (aa) | Proposed function | Closest homolog | Homolog (I/S, %) in | ||
|---|---|---|---|---|---|---|
Description, Origin, Accession number | I/Sb (%) | grh cluster | rub cluster | |||
769 | 230 | cyclase | hypothetical protein, Streptomyces fulvoviolaceus, WP_052425082 | 54/63 | – | RubK (53/63) |
768a | 656 | ABC transporter ATP-binding protein | multidrug ABC transporter ATP-binding protein, Actinopolymorpha alba, WP_020576731 | 70/83 | – | – |
767a | 577 | multidrug ABC transporter ATPase | multidrug ABC transporter ATPase, Streptomyces varsoviensis, WP_030881385 | 69/81 | – | – |
766a | 117 | MarR family transcriptional regulator | MarR family transcriptional regulator, Actinomadura macra, WP_067468911 | 45/63 | – | – |
765a | 72 | unknown | hypothetical protein, Streptomyces aurantiacus, WP_055507532. | 56/60 | – | – |
764a | 498 | transcriptional regulator | hypothetical protein, Streptomyces sp. NRRL WC-3742, WP_051836320 | 55/63 | GrhR2 (34/48) | |
763a | 533 | amide synthetase | hypothetical protein, partial, Streptomyces sp. NRRL WC-3742, WP_078910860 | 60/70 | – | – |
762a | 405 | 5-aminolevulinate synthase | AsuD2, Streptomyces nodosus subsp. asukaensis, ADI58646 | 77/85 | – | – |
761 | 515 | 5-aminolevulinate CoA ligase | AMP-dependent synthetase, Streptomyces uncialis, OKH94380 | 77/83 | – | – |
760 | 183 | unknown | hypothetical protein, Streptomyces prunicolor, WP_019061819 | 50/60 | – | – |
759 | 122 | unknown | hypothetical protein, Streptomyces fulvoviolaceus, WP_030615859 | 72/82 | GrhI (61/73) | – |
758 | 477 | oxygenase | hypothetical protein, Streptomyces yerevanensis, WP_033324694 | 72/82 | GrhO1 (72/80) | RubI (71/80) |
757 | 257 | 3-oxoacyl-ACP reductase | SDR family oxidoreductase, Streptomyces fulvoviolaceus, WP_030615854 | 83/92 | GrhO2 (73/81) | RubJ (83/91) |
756 | 325 | acetyltransferase | GrhJ, Streptomyces sp. CN48+, AIE76926 | 68/74 | GrhJ (67/73) | – |
755 | 540 | monooxygenase | hypothetical protein, Streptomyces prunicolor, WP_026151147 | 73/80 | GrhO5 (69/75) | RubL (73/80) |
754a | 161 | transcriptional regulator | putative transcriptional repressor GrhR3, Streptomyces sp. CN48+, AIE76928 | 76/88 | GrhR3 (76/88) | RubM (74/83) |
753 | 501 | monooxygenase | RubN, Streptomyces collinus, AAM97364 | 80/86 | GrhO6 (73/80) | RubN (80/86) |
752 | 325 | oxidoreductase | hypothetical protein, Streptomyces sp. TSRI0261, WP_073806081 | 86/93 | GrhO7 (78/89) | – |
751 | 343 | methyltransferase | hypothetical protein, Streptomyces fulvoviolaceus, WP_030615823 | 81/86 | GrhL (77/83) | – |
750 | 535 | monooxygenase | hypothetical protein, Streptomyces prunicolor, WP_019061807 | 74/82 | GrhO8 (70/79) | RubO (63/72)p |
749a | 534 | oxidoreductase | hypothetical protein, Streptomyces sp. TP-A0875, WP_053912978 | 74/80 | GrhO9 (71/79) | RubP (74/80) |
748 | 161 | unknown | hypothetical protein, Streptomyces prunicolor, WP_019061805 | 81/85 | GrhM (80/86) | RubQ (80/85) |
747 | 174 | unknown | hypothetical protein, Streptomyces fulvoviolaceus, WP_030615810 | 67/74 | GrhN (56/64) | RubW (64/74) |
746 | 623 | asparagine synthase | RubR, Streptomyces collinus, AAM97368 | 80/86 | GrhP (74/81) | RubR (80/86) |
745 | 669 | transcriptional regulator | RubS, Streptomyces collinus, AAM97369 | 63/75 | GrhR2 (43/56) | RubS (63/75) |
744 | 123 | cyclase | putative cyclaseI, Streptomyces collinus, AAG03065 | 83/88 | GrhQ (75/88) | RubE (83/88) |
743 | 143 | cyclase | cupin, Streptomyces sp. TSRI0261, OKJ01252 | 83/90 | GrhS (66/77) | RubD (79/85) |
742 | 424 | ketosynthase α subunit | type II polyketide synthase 4, Streptomyces sp., APD71740 | 89/95 | GrhA (85/91) | RubA (89/93) |
741 | 420 | ketosynthase β subunit | type II polyketide synthase 5, Streptomyces sp., APD71741 | 82/88 | GrhB (76/83) | RubB (79/85) |
740 | 87 | acyl carrier protein | acyl carrier protein, Streptomyces collinus, AAG03069 | 68/79 | GrhC (34/61) | RubC (68/79) |
739 | 398 | cyclase/reductase | hypothetical protein, Streptomyces prunicolor, WP_019061796 | 79/87 | GrhT (67/78) | RubF (78/85) |
738 | 249 | ketoreductase | SDR family oxidoreductase, Streptomyces prunicolor, WP_019061795 | 86/94 | GrhO10 (79/89) | RubG (86/93) |
737 | 108 | monooxygenase | hypthetical protein, Streptomyces collinus, AAG03072 | 88/93 | GrhU (75/84) | RubH (88/93) |
736 | 113 | unknown | hypothetical protein, Streptomyces fulvoviolaceus, WP_078655944 | 73/80 | GrhV (67/76) | RubT (70/81) |
735 | 417 | cytochrome P450 | cytochrome P450, Streptomyces fulvoviolaceus, WP_030615776 | 80/86 | GrhO3 (37/53) | RubU (80/86) |
734 | 301 | unknown | DUF1963 domain-containing protein, Streptacidiphilus carbonis, WP_042397320 | 78/85 | – | – |
733 | 155 | cupin | cupin, Streptomyces prunicolor, WP_019056246 | 93/97 | – | – |
732 | 322 | esterase | alpha/beta hydrolase, Actinobacteria bacterium OK074, KPI24488 | 83/88 | – | – |
731 | 313 | transcriptional regulator | transcriptional regulator, Streptomyces hokutonensis, WP_043260174 | 79/85 | – | – |
730a | 491 | unknown | dolichyl-phosphate-mannose-protein mannosyltransferase, Micromonospora auratinigra, SBT53146 | 57/67 | – | – |
729 | 42 | unknown | – | – | – | – |
728a | 333 | transcriptional regulator | LacI family transcriptional regulator, ‘Streptomyces humi’, WP_046734674 | 93/96 | – | – |