Fig. 2From: Complete genome sequence of Methanoculleus bourgensis strain MAB1, the syntrophic partner of mesophilic acetate-oxidising bacteria (SAOB)Phylogentic tree. Maximum likelihood tree highlighting the phylogenetic position of Methanoculleus bourgensis strain MAB1 within the family Methanomicrobiacaea. The 16S rRNA-based alignment was carried out using MUSCLE [53] and the phylogenetic tree was inferred from 1521 aligned characteristics of the 16S rRNA gene sequence using the maximum-likelihood (ML) algorithm [54] with MEGA 6.06 [55, 56]. Bootstrap analysis [57] with 100 replicates was performed to assess the support of the clustersBack to article page