Complete genome sequence of thermophilic Bacillus smithii type strain DSM 4216T

Table 1 Classification and general features of B. smithii DSM 4216^T according to MIGS standards

MIGS ID	Property	Term	Evidence code^a
	Classification	Domain Bacteria	TAS [30]
		Phylum Firmicutes	TAS [31–33]
		Class Bacilli	TAS [34, 35]
		Order Bacillales	TAS [36, 37]
		Family Bacillaceae	TAS [37, 38]
		Genus Bacillus	TAS [37–39]
		Species Bacillus smithii	TAS [2]
		Type strain: DSM 4216^T
	Gram stain	Positive^b	TAS [2]
	Cell shape	Rod	IDA (Fig. 1), TAS [2]
	Motility	Motile	TAS [2]
	Sporulation	Terminal or sub terminal, oval or cylindrical endospores, non-swollen to slightly swollen sporangia	IDA (Fig. 1), TAS [2]
	Temperature range	25–65 °C	TAS [2]
	Optimum temperature	55 °C	IDA
	pH range; Optimum	5.5–6.8; 6.5	TAS [2], IDA
	Carbon source	D-glucose, D-xylose, L-xylose, L-arabinose, D-ribose, glycerol, D-adonitol, D-fructose, L-sorbose, D-galactose, L-rhamnose, inositol, D-mannitol, sucrose, D-trehalose, xylitol, Methyl-α-D-glucopyranoside, esculin, salicin, D-maltose, D-turanose, D-lyxose, D-tagatose, D-arabitol, K-gluconate, K-5-ketogluconate	IDA(API), TAS [2]
MIGS-6	Habitat	Type strain: cheese. Other strains: evaporated milk, canned food, compost, hot spring soil, sugar beet juice from extraction installations.	TAS [2, 9–11]
MIGS-6.3	Salinity	Not in 3 % NaCl (w/v)	TAS [2]
MIGS-22	Oxygen requirement	Facultative anaerobe	TAS [2]
MIGS-15	Biotic relationship	Free-living	TAS [2]
MIGS-14	Pathogenicity	Non-pathogen	TAS [12, 13]
MIGS-4	Geographic location	USA	TAS [2, 40]
MIGS-5	Sample collection	~1946	TAS [2, 40]
MIGS-4.1	Latitude	Unknown
MIGS-4.2	Longitude	Unknown
MIGS-4.4	Altitude	Unknown

^aEvidence codes – IDA: Inferred from Direct Assay; TAS: Traceable Author Statement (i.e., a direct report exists in the literature); NAS: Non-traceable Author Statement (i.e., not directly observed for the living, isolated sample, but based on a generally accepted property for the species, or anecdotal evidence). These evidence codes are from the Gene Ontology project
^bAs described in the species description by Nakamura et al.: “Young cells of both groups were Gram positive. With increasing age the cells became Gram variable and finally Gram negative. The KOH and aminopeptidase tests were negative, as is typical for Gram-positive organisms.”

ISSN: 2524-6372