Figure 1

Maximum-likelihood phylogenetic tree of 16S rRNA genes for all Meganema isolates and closely related species in the LTP database (LTPs111) [ 10 ] constructed using the ARB software [ 13 ]. The 1342 bp long sequence fragment of the unique 16S rRNA gene copy in the genome is identical with the previously published 16S rRNA gene sequence for the Gr1^T strain (AF18048). A 20% maximum frequency filter was applied in order to remove hypervariable positions. Included are all uncultured clone sequences from the NCBI database which share ≥94% sequence similarity with the Gr1^T strain (all were ≥ 98%), with sequences from the same study clustered at ≥ 99% similarity and a representative included. Bootstrap values, calculated from 100 re-samplings, are indicated for branches with > 50% support. Scale bar represents substitutions per nucleotide. The family Fusobacteriaceae was used as the out-group.