Figure 1.
From: The DOE-JGI Standard Operating Procedure for the Annotations of Microbial Genomes

Data flow for gene prediction in the DOE-JGI MAP. Nucleotide sequences are annotated using tools to predict repeats (CRISPR) and RNA genes. Subsequently protein-coding genes are predicted using either GeneMark or Metagene. The consolidated results are then used to create a Genbank file, which is uploaded into the IMG/ER database.