Phylogenetic tree highlighting the position of A. flavithermus subsp. yunnanensis E13T relative to other typical solvent-tolerant bacteria. The 16S rRNA sequences were aligned using ClustalX2, and phylogenetic inferences obtained using the neighbor-joining method with the MEGA program. Species and GenBank accession numbers are indicated. Bootstrap values based on 1,000 replicates show the robustness of the branching. Scale bar represents 0.02 substitutions per nucleotide position. Strains with genome sequencing projects registered in GenBank are shown in bold.