Table 5. Preliminary genes involved in the hydrogen production pathway according to the MIGS recommendations [8]
Protein product | Gene locus | Molecular mass (kDa) | Description |
|---|---|---|---|
FdhD | EntIITBT8DRAFT_3548 | 29.9 | formate dehydrogenase family accessory protein (protease) |
FdhE | EntIITBT8DRAFT_3551 | 89.4 | formate dehydrogenase, alpha subunit, proteobacterial-type [EC:1.2.1.2 ] |
FdhF | EntIITBT8DRAFT_1016 | 79.3 | formate dehydrogenase, alpha subunit, archaeal-type (EC:1.2.1.2) |
HycA | EntIITBT8DRAFT_2685 | 17.5 | Transcriptional repressor of hyc and hyp operons |
HycD | EntIITBT8DRAFT_2682 | 33.0 | Formate hydrogenlyase subunit 4 |
HycE | EntIITBT8DRAFT_2681 | 64.8 | Ni, Fe-hydrogenase III large subunit |
HycG | EntIITBT8DRAFT_2679 | 28.0 | Ni,Fe-hydrogenase III small subunit |
HycH | EntIITBT8DRAFT_2678 | 15.2 | Formate hydrogenlyase maturation protein |
HycI | EntIITBT8DRAFT_2677 | 16.2 | hydrogenase maturation protease [EC:3.4.23.51] |
? | EntIITBT8DRAFT_2511 EntIITBT8DRAFT_2680 | 23.0 | Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase subunit (chain I) |
? | EntIITBT8DRAFT_2683 | 63.5 | Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit |
HycB?? | EntIITBT8DRAFT_2684 | 21.7 | Fe-S-cluster-containing hydrogenase components 2 |
HypA | EntIITBT8DRAFT_2686 | 13.1 | hydrogenase nickel insertion protein |
HypB | EntIITBT8DRAFT_2687 | 31.1 | hydrogenase accessory protein |
HypC/HupF | EntIITBT8DRAFT_2688 | 9.65 | hydrogenase assembly chaperone HypC/HupF |
HypD | EntIITBT8DRAFT_2689 | 41.1 | hydrogenase expression/formation protein HypD |
HypE | EntIITBT8DRAFT_2690 | 35.3 | hydrogenase expression/formation protein HypE |
HypF | EntIITBT8DRAFT_2672 | 80.5 | [NiFe] hydrogenase maturation protein HypF |
HoxN/HupN/NixA family | EntIITBT8DRAFT_2671 | 36.7 | high-affinity nickel-transporter |