Phylogenetic tree highlighting the position of Dehalobacter restrictus relative to phylogenetically closely related organisms. 16S rRNA sequences were retrieved from Genbank (NCBI), and accession numbers are given in parentheses. Strains from which a full genome sequence are available are indicated with an asterisk. Phylogenetic analysis was done using the MEGA5 software package . Sequences were aligned using the MUSCLE algorithm before a neighbor joining tree was constructed and validated with 1,000 bootstraps [21,22]. The reference bar indicates 2% sequence divergence.