Maximum likelihood phylogenetic tree was generated using PHYML v2.2.4  based on 16S rRNA sequences highlighting the position of S. rugosus CDC 945T relative to the other type strains of mycolic acid containing genera in the suborder Corynebacterineae. GenBank accession numbers are listed after the name. The tree was inferred from 1,468 bp positions aligned using Clustal W  in MEGA v4 . Numbers at the branch nodes are support values from 1,000 bootstrap replicates if equal to or greater than 70%. The scale bar indicates substitutions per site. The tree was rooted with Streptomyces coelicolor. Lineages with type strain genome sequencing projects registered in GOLD  are shown in blue, published genomes in bold.