Figure 3.
Reconstruction of the rTCA cycle and related gene clusters in C. mediatlanticus strain TB-2T. A) rTCA cycle. Enzymes are identified by the corresponding gene locus in the C. mediatlanticus genome (CMTB2_gene number). B) Structure of the gene clusters encoding for enzymes involved in rTCA cycle. ORF present within the same clusters are shown in light gray. The distance between clusters is reported in thousands of base pairs (kbp). Genes are oriented according to their direction and drawn to scale. frdBA: fumarate reductase; citE: citril-CoA lyase; pyk: pyruvate kinase; idh1: monomeric isocitrate dehydrogenase; mdh2: malate dehydrogenase; fumAB: fumarate hydratase; sucCD: succinyl-CoA synthetase; oorDABC: 2-oxoglutarate ferredoxin synthase; pycAB: pyruvate carboxylase; ppsA: pyruvate water dikinase; acb: bifunctional aconitate hydratase/methyl isocitrate dehydratase; acnB: aconitate hydratase; pckA: phosphoenol pyruvate carboxy kinase; porGDAB: pyruvate ferredoxinoxido reductase/synthase; aclBA: ATP-citrate lyase.