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Table 6. Metatranscriptome statistics

From: Metagenomes and metatranscriptomes from the L4 long-term coastal monitoring station in the Western English Channel

  Jan 3pm Jan 7pm Apr 4pm Apr 10pm Aug 27 4pm Aug 27 10pm Aug 28 4am
No. Original cDNA Sequences 139,880 130,826 124,925 147,492 139,375 193,254 154,865
No. of sequences following filtering*** 94,024 106,864 84,916 109,577 87,799 118,360 111,568
No. mRNA following removal of rRNA 61,831 96,026 41,378 53,413 33,149 51,829 55,006
Predicted ORFs (>40aa pORFs) 143,169 211,374 81,642 107,699 77,985 66,529 159,909
No. of pORF clusters (95%) 98,871 78,278 35,648 51,088 28,167 24,136 68,080
No. of pORF singletons (95%) 82,464 54,870 25,925 38,960 19,600 17,177 50,246
No. of pORF ‘families’ (60%) 84,598 45,049 19,131 37,628 15,146 12,735 41,480
No. of pORF singletons (60%) 76,655 30,720 13,869 30,919 9,857 9,134 32,662
Resampled pORFs (66529)        
No. of pORF clusters (95%) (66529) 31026 50354 30334 34217 24848 24136 33191
No. of pORF singletons (95%) (66529) 23038 43687 22394 26840 17373 17177 25636
Good’s Coverage (66529) 65.37 34.33 66.34 59.66 73.89 74.18 61.47
No. mRNA seqs with functional annotation 11,513 31,990 8,845 16,315 11,720 5,907 15,384
No. mRNA seqs without functional annotation 50,318 64,036 32,533 37,098 21,429 45,922 39,622
Percent DNA seqs without functional annotation 81% 67% 79% 69% 65% 89% 72%
No. mRNA seqs with taxonomic annotation 29,521 30,778 20,899 26,398 15,456 29,605 38,304
Resampled sequencing effort (15,456)        
Number of archaeal sequences (15,456) 625 49 1 16 4 4 11
Number of bacterial sequences (15,456) 13,633 11,926 13,702 8,449 14,469 15,071 14,803