Table 6. Metatranscriptome statistics

No. Original cDNA Sequences

139,880

130,826

124,925

147,492

139,375

193,254

154,865

No. of sequences following filtering***

94,024

106,864

84,916

109,577

87,799

118,360

111,568

No. mRNA following removal of rRNA

61,831

96,026

41,378

53,413

33,149

51,829

55,006

Predicted ORFs (>40aa pORFs)

143,169

211,374

81,642

107,699

77,985

66,529

159,909

No. of pORF clusters (95%)

98,871

78,278

35,648

51,088

28,167

24,136

68,080

No. of pORF singletons (95%)

82,464

54,870

25,925

38,960

19,600

17,177

50,246

No. of pORF ‘families’ (60%)

84,598

45,049

19,131

37,628

15,146

12,735

41,480

No. of pORF singletons (60%)

76,655

30,720

13,869

30,919

9,857

9,134

32,662

Resampled pORFs (66529)

No. of pORF clusters (95%) (66529)

31026

50354

30334

34217

24848

24136

33191

No. of pORF singletons (95%) (66529)

23038

43687

22394

26840

17373

17177

25636

Good’s Coverage (66529)

65.37

34.33

66.34

59.66

73.89

74.18

61.47

No. mRNA seqs with functional annotation

11,513

31,990

8,845

16,315

11,720

5,907

15,384

No. mRNA seqs without functional annotation

50,318

64,036

32,533

37,098

21,429

45,922

39,622

Percent DNA seqs without functional annotation

81%

67%

79%

69%

65%

89%

72%

No. mRNA seqs with taxonomic annotation

29,521

30,778

20,899

26,398

15,456

29,605

38,304

Resampled sequencing effort (15,456)

Number of archaeal sequences (15,456)

625

49

1

16

4

4

11

Number of bacterial sequences (15,456)

13,633

11,926

13,702

8,449

14,469

15,071

14,803