Fig. 5

The distribution of PAH-degrading genes in the NCBI complete genome database. From the outside to the inside: (1) The color in the circle indicates the location of identified PAH-degrading genes; (2) The pink circle, blue triangle, and green square indicate nah, nar, and nid and phd gene cluster, respectively. (3) The red tag represents the 47 identified PAH-degrading bacteria, while the blue tag denotes the 28 predicted PAH-degrading bacteria by random forest analysis. Six pure strains selected for experimental verification are depicted with yellow shades. (4) The species affiliated with the family Mycobacteriaceae are distinguished by orange branch dashed lines, whereas the branch dashed lines of Gram-negative and Gram-positive strains are blue and red, respectively. Notably, the nahE identified in Mycobacteriaceae was misclassification