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Table 1 Antimicrobial resistance genes found in the unbinned-assembled reads (from heavy and light fractions) and MAG-1

From: Identification of diverse antibiotic resistant bacteria in agricultural soil with H218O stable isotope probing combined with high-throughput sequencing

Antimicrobial

Class

light

heavy

MAG-1

Gene (Similarity (%) / Reference*)

Light

Heavy

MAG-1

Butirosin

Aminoglycoside

 

X

X

 

aph(3’)-IIc (85.46% / AM743169)

aph(3’)-IIc (85.46% / AM743169)

Paromomycin

 

X

X

 

Kanamycin

 

X

X

 

Neomycin

 

X

X

 

Chloramphenicol

Amphenicol

 

X

X

 

OqxB (76.28% / EU370913)

OqxB (76.28% / EU370913)

Benzylkonium chloride

Quaternary ammonium compound

 

X

X

 

Cetylpyridinium chloride

Quaternary ammonium compound

 

X

X

 

Ciprofloxacin

Quinolone

 

X

X

 

Nalidixic acid

Quinolone

 

X

X

 

Trimethoprim

Folate pathway antagonist

X

X

X

dfrB3 (90.14% / X72585)

OqxB (76.28% / EU370913)

OqxB (76.28% / EU370913)

Unknown beta-lactam

Beta-lactam

X

X

 

blaTEM-181 (99.86% / KM977568)

blaL1 (85.84% / EF126059)

 
  1. * AM743169: Stenotrophomonas maltophilia strain K279a; EU370913: Escherichia coli plasmid pOLA52; X72585: Klebsiella aerogenes plasmid R751 genes from integron element; KM977568: Cloning and transformation vector pUC-3GLA; EF126059: Stenotrophomonas maltophilia strain K1 (beta-lactamase gene)