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Fig. 1 | Environmental Microbiome

Fig. 1

From: Thrive or survive: prokaryotic life in hypersaline soils

Fig. 1

General scheme of the methodology used. First, soil was dried to approximately 50% water-holding capacity. Then, soil subsamples were subjected to each of the four treatments (Light; Dark; Light + 3-(3,4-dichlorophenyl)-1,1-dimethylurea, DCMU; Dark + DCMU) in triplicate. Sterile deionized water was used for the controls. Tubes were placed in an incubator at 37 °C for 21 days in either Light or Dark conditions. After DNA extraction, the DNA-SIP procedure was performed as described by Neufeld et al. [54], with slight modifications (see methods section). Archaeal and bacterial 16S rRNA genes in each of the 14 fractions obtained were quantified by real-time PCR. DNA fractions were pooled according to their buoyant densities into three groups: light DNA (L, comprising individual fractions with buoyant densities in the range 1.690–1.729 g/ml, coinciding to that of controls and therefore corresponding to unlabeled DNA), medium heavy DNA (MH, 1.730–1.749 g/ml) and heavy DNA (H, 1.750–1.780 g/ml). The binned density fractions were analyzed for 16S rRNA genes

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