Fig. 4From: Metagenomics and metabarcoding experimental choices and their impact on microbial community characterization in freshwater recirculating aquaculture systemsTaxonomic units unique for specific primers and/or compartmens according to 16S sequencing. Permanova coefficients indicate which ASVs are most characteristic for (but not necessarily most abundant in) a particular compartment. Uppercase captions indicate the lowest classified order (O = Order, F = Family, G = Genus). Taxonomic units containing aquaculture pathogens are marked with an asterisk. Primer pair differences (different emerge at the ASV level. We found that water and biofilm samples from the same farm and circuit differ in differentially enriched ASVs, which is vital for understanding taxa diversity and functional services within different sample types. Notably, both primers could identify pathogenic groups within the farms, e.g., Chryseobacterium, Flavobacterium, and AeromonasBack to article page