Fig. 1

Overview of the MarineMetagenomeDB construction workflow. A Metadata retrieval for both SRA and MG-RAST samples was achieved using different pipelines, as explained in the text. Followed by the removal of non-WGS and non-marine samples. B Standardization of attributes. Sample attributes including ‘Dates’, ‘Location’ and ‘Depth’ were standardized (C) Identification of marine terms. Marine terms were adapted from Marine Biome, Environmental Material, and Geographic Feature of the Environmental Ontology (ENVO). An example of a collection of terms grouped as ‘constructed structures’ (labeled with a double asterisk) can be found in the Additional file (see Additional file 3: Table S3) (‘MarMDB_constructed_structures’) (D) Merging SRA and MG-RAST dataset. The MG-RAST attributes were adapted to the SRA metadata standard. E MarineMetagenomeDB is made available online through a shiny web implementation. Adapted from [17, 18]