Metagenomic community composition and resistome analysis in a full-scale cold climate wastewater treatment plant

Table 2 Description of primers and probes used for quantitative PCR

Gene	Primer name	Sequence (5′–3′)	Product size (bp)	References
intI1	IntI1-LC1	GCCTTGATGTTACCCGAGAG		Barraud et al. [22]
	IntI1_LC5	GATCGGTCGAATGCGTGT	196
	IntI1_probe	(FAM) ATTCCTGGCCGTGGTTCTGGGTTTT (/ZEN, IABkFQ)
intI2	IntI2_LC2	TGCTTTTCCCACCCTTACC		Barraud et al. [22]
	IntI2_LC3	GACGGCTACCCTCTGTTATCTC	195
	IntI2_probe	FAM-TGGATACTCGCAACCAAGTTATTTTTACGCTG (/ZEN, IABkFQ)
intI3	IntI3_LC1	GCCACCACTTGTTTGAGGA		Barraud et al. [22]
	IntI3_LC2	GGATGTCTGTGCCTGCTTG	138
	IntI3_probe	(FAM) CGCCACTCATTCGCCACCCA (/ZEN, IABkFQ)
uidA	784F	GTGTGATATCTACCCGCTTCGC		Frahm and Obst [23]
	866R	AGAACGGTTTGTGGTTAATCAGGA	84
	EC807	(FAM) TCGGCATCCGGTCAGTGGCAGT (/ZEN, IABkFQ)
16s rRNA gene	Bac1055YF	ATGGYTGTCGTCAGCT		Ritalahti et al. [26]
	Bac1392R	ACGGGCGGTGTGTAC	~ 320 bp
	Bac1115P	(FAM) CAACGAGCGCAACCC (/ZEN, IABkFQ)

ISSN: 2524-6372