Fig. 3

CK prevents age-associated decrease in bacilli content in the bacterial community. Bacterial DNA was extracted from indicated genotypes at the seedling (15-day old, indicated with "s" and white bars) and mature plant (45-day old, indicated with "m" and gray bars) stages. Bacilli amounts were estimated by qPCR of the bacillus 16S rRNA gene, normalized to qPCR of the total 16S rRNA gene content, and expressed as % of the total bacterial community. A Comparison of bacilli content in the background M82, the high CK content IPT, the high CK sensitivity clau, and the low CK content CKX, at both seedling and mature stages. B Comparison of the different genotypes at the seedling stage, all points shown. C Comparison of the different genotypes at the mature stage, all points shown. Bars depict mean ± SE. Different letters indicate statistically significant differences in an unpaired two-tailed t-test with Welch's correction, N > 10. A p < 0.048, B p < 0.043, C p < 0.018