Seasonality, shelf life and storage atmosphere are main drivers of the microbiome and E. coli O157:H7 colonization of post-harvest lettuce cultivated in a major production area in California

Table 1 Core microbiomes of lettuce after processing and prior to storage, and during MAP storage

	Taxa	Relative abundance (%)
Pre-storage	Erwiniaceae Pantoea	1.77	16.53
	Erwiniaceae Pantoea agglomerans	14.76
	Pseudomonadaceae Pseudomonas fluorescens	8.92	8.92
	Noncore taxa	74.56
6 °C storage	Erwiniaceae	1.53	33.96
	Erwiniaceae Pantoea	3.25
	Erwiniaceae Pantoea agglomerans	29.17
	Pseudomonadaceae Pseudomonas	0.51	34.13
	Pseudomonadaceae Pseudomonas moraviensis	14.37
	Pseudomonadaceae Pseudomonas fluorescens	10.38
	Pseudomonadaceae Pseudomonas koreensis	7.91
	Pseudomonadaceae Pseudomonas granadensis	0.95
	Noncore taxa	31.91
24 °C storage	Erwiniaceae	2.07	40.59
	Erwiniaceae Pantoea	4.24
	Erwiniaceae Pantoea agglomerans	32.25
	Erwiniaceae Pantoea vagans	0.12
	Erwiniaceae Erwinia	0.86
	Erwiniaceae Erwinia persicina	0.84
	Erwiniaceae Erwinia gerundensis	0.20
	Enterobacteriaceae	1.22	19.33
	Enterobacteriaceae Enterobacter cloacae	11.49
	Enterobacteriaceae Leclercia adecarboxylata	6.61
	Pseudomonadaceae Pseudomonas moraviensis	3.92	7.57
	Pseudomonadaceae Pseudomonas koreensis	2.05
	Pseudomonadaceae Pseudomonas fluorescens	1.60
	Noncore taxa	32.52

Core microbiomes were determined based on all DA and TT samples, inoculated and noninoculated

ISSN: 2524-6372