Fig. 1From: The treasure inside barley seeds: microbial diversity and plant beneficial bacteriaMicrobial diversity of the endophytic seed microbiome varied between seven different barley genotypes. Golden Promise and Morex were chosen as reference genotypes. HOR7985, BCC1415, BCC436, BCC768 and BCC1589 represent the genetic diversity within a 224 spring barley accession set [14, 24]. The microbial alpha-diversity indices Species richness (a) and Shannon diversity index (b) varied depending on the plant genotype (ANOVA for Species richness p ≤ 0.001 and Shannon index p ≤ 0.01). Values of alpha-diversity indices including statistics can be seen in Additional file 1: Table S2. Beta-diversity of the endophytic seed microbiome was visualized by NMDS (c). The microbiome composition based on Bray–Curtis community dissimilarities (ASVs obtained from 16S rRNA gene amplicon sequencing) and was assessed from DNA of surface-sterilized barley seeds of seven different genotypes. ANOSIM verified significant differences between the genotypes (p ≤ 0.001)Back to article page