Coupled DNA-labeling and sequencing approach enables the detection of viable-but-non-culturable Vibrio spp. in irrigation water sources in the Chesapeake Bay watershed

Table 2 Prevalence of V. parahaemolyticus, V. cholerae and V. vulnificus in nontraditional irrigation water samples processed using culture methods with two different types of enrichment media

Number of Samples (%) Positive for *Vibrio* spp.
Sampling sites	*V. parahaemolyticus*		*V. cholerae*		*V. vulnificus*
Sampling sites	APW	EPW	APW	EPW	APW	EPW
Reclaimed water (n = 8)	1 (12.5%)	1 (12.5%)	1 (12.5%)	1 (12.5%)	1 (12.5%)	0
Tidal brackish water (n = 8)	3 (37.5%)	2 (25%)	6 (50%)	3 (37.5%)	0	0
Nontidal freshwater (n = 6)	0	0	0	1 (16.67%)	0	0
Pond water (n = 8)	0	0	0	0	0	0

APW Alkaline peptone water enrichment. EPW Estuarine peptone water enrichment

ISSN: 2524-6372