Fig. 2
From: Trichomes form genotype-specific microbial hotspots in the phyllosphere of tomato
Micrographs of tomato leaves (a-d) as well as observed alpha (e) and beta (f) diversity of tomato-associated bacterial communities. Leaf of Solanum lycopersicum LA4024 before (a) and after (b) brushing to isolate trichomes. Leaf of S. habrochaites LA1777 before (c) and after (d) brushing to isolate trichomes. Note that glandular trichomes are visible as white dots in (a) and (c) before they have been removed by brushing. All scalebars represent 5 mm. The bacterial richness was assessed with the Shannon diversity index (e). Different sample types and tomato lines were separately assessed and the significantly differences tested by Kruskal-Wallis (indicated by letters). Beta diversity was accessed by visualizing a Bray Curtis distance matrix in a non-metric multidimensional scaling (NMDS) plot showing bacterial differences between each sample (f). Shapes represent tomato genotypes (circle: LA1777, square: LA4024); colors represent sample type (blue: Leaves, green: Trichomes, and red: brushed leaves without trichomes)