Impact of process temperature and organic loading rate on cellulolytic / hydrolytic biofilm microbiomes during biomethanation of ryegrass silage revealed by genome-centered metagenomics and metatranscriptomics

Irena Maus; Michael Klocke; Jaqueline Derenkó; Yvonne Stolze; Michael Beckstette; Carsten Jost; Daniel Wibberg; Jochen Blom; Christian Henke; Katharina Willenbücher; Madis Rumming; Antje Rademacher; Alfred Pühler; Alexander Sczyrba; Andreas Schlüter

doi:10.1186/s40793-020-00354-x

Environmental Microbiome

Table 1 Taxonomic affiliations and characteristics of selected metagenome-assembled genomes (MAGs) most abundant in HR biofilms

From: Impact of process temperature and organic loading rate on cellulolytic / hydrolytic biofilm microbiomes during biomethanation of ryegrass silage revealed by genome-centered metagenomics and metatranscriptomics

MAG	Taxonomic affiliation¹					MAG features
MAG	Phylum	Class	Order	Family	Genus	Comleteness² [%]	Contamination² [%]	Size [bp]	GC content [%]	No. of genes	No. of rrn genes	No. of tRNA genes
Bacteria
13	Firmicutes	Unknown	Unknown	Unknown	Unknown	29.74	3.43	1,239,774	44.81	1159	3	31
17*	Bacteroidetes	Bacteroidia	Bacteroidales	Bacteroidaceae	Prevotella sp.	68.02	8.98	2,689,597	48.90	2294	2	39
18*	Firmicutes	Clostridia	Lachnospirales	Lachnospiraceae	Acetivibrio sp.	68.50	3.65	2,855,089	39.03	2917	n/d³	15
40*	Firmicutes	Clostridia	Lachnospirales	Lachnospiraceae	Herbinix sp.	68.42	7.66	1,849,940	36.65	1755	n/d	8
48	Firmicutes	Clostridia	Lachnospirales	Lachnospiraceae	Herbinix sp.	26.48	2.63	770,644	37.4	614	n/d	3
50	Firmicutes	Clostridia	Lachnospirales	Defluviitaleaceae	Unknown	51.74	6.14	1,725,349	31.02	1775	n/d	31
68	Firmicutes	Clostridia	Lachnospirales	Lachnospiraceae	Herbinix sp.	52.17	4.77	2,358,488	46.61	2310	2	27
84	Firmicutes	Clostridia	Lachnospirales	Unknown	Unknown	35.22	5.14	1,970,476	38.81	1672	2	17
85	Firmicutes	Unknown	Unknown	Unknown	Unknown	80.11	3.08	2,011,311	47.98	2076	n/d	33
106	Firmicutes	Clostridia	Clostridiales	Clostridiaceae	Clostridium sp.	33.09	4.39	883,787	31.79	812	n/d	17
107*	Firmicutes	Clostridia	Clostridiales	Clostridiaceae	Clostridium sp.	50.06	6.97	1,539,556	29.37	1648	n/d	17
108*	Bacteroidetes	Bacteroidia	Bacteroidales	Unknown	Unknown	79.01	8.21	2,032,215	47.60	2025	1	29
109	Bacteroidetes	Bacteroidia	Bacteroidales	Dysgonomonadaceae	Proteiniphilum sp.	63.52	7.19	2196,928	45.07	2196	1	27
132	Firmicutes	Clostridia	Unknown	Unknown	Unknown	26.26	2.37	1,284,977	37.03	1286	n/d	16
134*	Firmicutes	Clostridia	Lachnospirales	Lachnospiraceae	Kineothrix sp.	78.46	9.27	2,788,291	40.86	2946	n/d	31
145	Firmicutes	Bacilli	Lactobacillales	Aerococcaceae	Jeotgalibaca sp.	63.37	9.48	2,346,037	46.04	2647	n/d	14
146	Firmicutes	Bacilli	Unknown	Unknown	Unknown	33.22	0.00	861,844	38.49	705	1	13
Archaea
111	Euryarchaeota	Methanobacteria	Methanobacteriales	Methanobacteriaceae	Methanobacterium sp.	73.74	8.57	2,134,563	37.98	2406	2	51

¹ GTDB-Tk based classification
² For details, refer to [22] and Additional file 4
³ n/d = not detected
* MAGs which showed increased transcriptional activity in responce to the organic loading rate (OLR)

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