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Fig. 6 | Environmental Microbiome

Fig. 6

From: Impact of process temperature and organic loading rate on cellulolytic / hydrolytic biofilm microbiomes during biomethanation of ryegrass silage revealed by genome-centered metagenomics and metatranscriptomics

Fig. 6

Alteration in transcriptional activity of 78 selected metagenome-assembled genomes (MAGs) detected in HR biofilms at mesophilic (M) and thermophilic (T) process temperature in response of the increase of organic loading rate (OLR) from 500 to 1500 g ryegrass silage. PCA plots are shown for principle component analyses of scaled and centered rlog transformed metatrabscriptome read counts mapped on MAGs (a). Transcripts were normalized on the total number of sequencing reads, assuming equal MAG abundance, and were averaged for biological replicates (b). Green, mesophilic HR biofilms; blue, thermophilic HR biofilms. For further details on sample denomination, refer to Figs. 2 and 3. Alteration in transcript abundance of MAGs selected in response of the increase of OLR from 500 to 1500 g ryegrass silage at mesophilic (c) and thermophilic (d) process temperature. Colors of the MAGs indicate taxonomic affiliation (green, Firmicutes; blue, Bacteroidota; violet, Archaea; pink, Cloacimonadota; light green, Fibrobacterota; dark red, Spirochaetota; red, unknown affiliation). * indicate MAGs with completeness above 50% and contamination rate less than 10% as listed in Table 1

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