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Table 5 Abundant microbial taxa in pellet and supernatant fractions from subway air samples (MetaSUB method)

From: Performance evaluation of a new custom, multi-component DNA isolation method optimized for use in shotgun metagenomic sequencing-based aerosol microbiome research

SupernatantPellet
PhylumPrevalenceabundancePhylumPrevalenceabundance
meanTotalmeanTotal
Actinobacteria3.5947951.21%Actinobacteria4.010,65353.53%
Proteobacteria3.114,45328.87%Proteobacteria3.415,89424.50%
Bacteroidetes2.831397.10%Basidiomycota3.77175.42%
Firmicutes2.538164.23%Ascomycota4.221095.23%
Deinococcus-Thermus3.21843.06%Bacteroidetes2.932024.44%
Basidiomycota1.52901.86%Firmicutes2.638742.68%
Ascomycota2.412311.62%Deinococcus-Thermus3.72142.05%
Cyanobacteria2.84311.47%Cyanobacteria3.65571.03%
Euryarchaeota1.52600.17%Euryarchaeota2.13670.66%
Acidobacteria3.41030.10%Acidobacteria4.41320.11%
FamilyPrevalenceabundanceFamilyPrevalenceabundance
meanTotalmeanTotal
Micrococcaceae3.673511.27%Micrococcaceae3.979514.70%
Nocardioidaceae3.12369.32%Nocardioidaceae3.02228.26%
Microbacteriaceae3.49215.24%Geodermatophilaceae2.9974.65%
Sphingomonadaceae3.811195.20%Microbacteriaceae3.59584.53%
Geodermatophilaceae3.71214.60%Sphingomonadaceae4.312604.22%
Moraxellaceae2.97944.14%Intrasporangiaceae3.81994.20%
Hymenobacteraceae3.51503.93%Moraxellaceae2.67172.77%
Intrasporangiaceae3.71923.81%Corynebacteriaceae4.28812.61%
Corynebacteriaceae3.77893.77%Rhodobacteraceae4.316982.33%
Deinococcaceae4.01173.03%Hymenobacteraceae3.81632.15%
GenusPrevalenceabundanceGenusPrevalenceabundance
meanTotalmeanTotal
Arthrobacter3.33206.31%Arthrobacter3.73577.74%
Sphingomonas3.75124.40%Micrococcus3.9394.19%
Nocardioides3.01273.87%Sphingomonas4.15603.40%
Hymenobacter3.4893.85%Nocardioides2.91203.14%
Corynebacterium3.87673.73%Blastococcus3.5393.13%
Psychrobacter2.51433.24%Corynebacterium4.28542.59%
Deinococcus4.01173.03%Marmoricola5.8232.56%
Friedmanniella6.0182.85%Friedmanniella6.0182.38%
Blastococcus4.1452.60%Psychrobacter2.31332.37%
Micrococcus4.6462.31%Hymenobacter3.2842.04%
SpeciesPrevalenceabundanceSpeciesPrevalenceabundance
meanTotalmeanTotal
Deinococcus marmoris6.062.07%Arthrobacter sp. H416.062.07%
Arthrobacter sp. Leaf2346.061.91%Micrococcus luteus6.061.96%
Marmoricola sp. Leaf4466.061.31%Rubrobacter aplysinae6.061.52%
Friedmanniella flava6.061.05%Arthrobacter sp. Leaf2346.061.51%
Friedmanniella sagamiharensis6.061.03%Marmoricola sp. Leaf4466.061.42%
Cutibacterium acnes6.061.02%Blastococcus sp. DSM 442686.061.32%
Mrakia frigida6.061.02%Deinococcus marmoris6.061.27%
Blastococcus sp. DSM 442686.061.02%Arthrobacter agilis6.060.95%
Micrococcus luteus6.061.01%Friedmanniella flava6.060.89%
Arthrobacter sp. H416.060.98%Stereum hirsutum6.060.86%
  1. Top ten microbial phyla, families, genera and species in the intermediate pellet (N = 6) and supernatant (N = 6) fractions from subway air samples (N = 6) processed with the MetaSUB method