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Fig. 4 | Environmental Microbiome

Fig. 4

From: The impact of sequencing depth on the inferred taxonomic composition and AMR gene content of metagenomic samples

Fig. 4

The effect of normalization on the most common AMR gene allelic variants from each sample. Shown are the top 20 AMR gene allelic variants from each sample (effluent, pig caeca and upstream sediment), and the effect of different normalisations (left: raw count, middle: normalisation by gene length, right: further normalisation by Thermus thermophilus count). Arrows show the changing rank of each variant with normalisation. Note that a different x-axis is used for upstream sediment in all three panels. Asterisks denote AMR allelic variants that do not have a “protein homolog” detection model in CARD (see Methods: ‘AMR gene profiling’)

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