Fig. 3

Mn²⁺ and Cd²⁺ removed by P. manganicus JH-7^T. Control stands for null LB medium. Strain JH-7^T was incubated until OD₆₀₀ reach 1.0, and then amended with 5000 μmol/L MnCl₂ (a) and 100 μmol/L CdCl₂ (b), respectively. The cultures were removed at 24 h intervals. After centrifuging at 12,000 rpm for 10 min, the supernatant was used to determine the residual concentration of Mn²⁺ and Cd²⁺ by the atomic absorption spectrometry AAS (AAS; 986A, Beijing Puxi General Instrument 197 Co., Beijing, China). Bars represent the mean ± SD of three biological replicates