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Table 5 Metagenome statistics

From: Functional and taxonomic classification of a greenhouse water drain metagenome

Label

Metagenome Label

Comment

Libraries used

Drain-A and Drain-B

We performed the assembly using all the reads of the two libraries that passed quality filters.

Assembly tool(s) used

IDBA-UD and MetaSpades and merged with nucmer

20–125 of k-mer length (IDBA-UD) 21–121 (MetaSpades)

Number of contigs after assembly

7003

These numbers correspond to the best assembly merged using nucmer.

Number of singletons after assembly

N/A

MetaSpades and IDBA-UD were used in pre-correction mode to discard singletons k-mers.

Total bases assembled

859,091,400

Total base pairs in the assembly.

Contig n50

4246

 

% of Sequences assembled

97%

The fraction of the input data in the assembly.

Measure for % assembled

79%

The method used for calculating % assembled was determinate by read mapping using BWA (default parameters) against final assembly and considering the total reads (7,208,266 reads)